Influence of organic modifiers on the rentention behaviour in reversed-phase liquid chromatography and its consequences for gradient elution

Shoenmakers, Peter J.; Billiet, H.A.H.; Galan, L. de

doi:10.1016/s0021-9673(00)85604-6

Cited by 393 publications

(89 citation statements)

References 10 publications

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“…[16,17] Because log k 0 values in pure water can be very large and, consequently, determination of exact peak maxima difficult, log k 0 is usually measured in methanol-water or acetonitrile-water mixtures. For a given stationary phase in reversed-phase HPLC, the relationship between the capacity factor of a solute and the volume fraction (j) of the organic modifier in the mobile phase is given by [18] :…”

Section: Introductionmentioning

confidence: 99%

Experimental hydrophobicity parameters of perfluorinated alkylated substances from reversed-phase high-performance liquid chromatography

et al. 2012

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Environmental context. Perfluorinated compounds are synthetic chemicals shown to be present in the blood of humans. To study how these contaminants get into our blood requires a good understanding of their physicochemical properties. We describe an alternative way to obtain values for how perfluorinated compounds distribute between water and fatty phases (mimicking e.g. gut content and gut wall), which is essential information for modelling and understanding the environmental fate of these chemicals.Abstract. Capacity factors of perfluorinated alkylated substances were obtained from isocratic reversed-phase highperformance liquid chromatography-mass spectrometry experiments at different organic modifier strengths of the mobile phase. The resulting capacity factor v. modifier strengths plots were extrapolated to obtain capacity factors at 100 % water (k 0 ) that can serve as indicators of the hydrophobicity of the perfluorinated acids. Values of log k 0 were shown to increase linearly with the number of CF 2 units in the fluorinated alkyl chain.

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Section: Introductionmentioning

confidence: 99%

Experimental hydrophobicity parameters of perfluorinated alkylated substances from reversed-phase high-performance liquid chromatography

et al. 2012

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“…Various theories of gradient elution were proposed [173][174][175][176][177][178][179][180] [1][2][3]. However, these theories are only valid as long as the system dwell volume (V d ), also known as gradient delay volume, is taken into account.…”

Section: Gradient Delay Volumementioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

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“…By extension of classical thermodynamics to noninteracting binary solvent mixtures, the capacity factor can be described as a quadratic func- (2) where A , are the regression coefficients. If the volume fraction is a constrained parameter (e.g., 4, = 1 -+,), this expression can be simplified as follow^ [39-411 (3) In order to reduce the number of experiments required for optimization, this quadratic model is frequently approximated by a linear equation [35,[42][43][44][45][46][47][48] ln k = A , +A,+, + All+;. Ink = A , + A 1 + l .…”

Section: Univariate Optimizationmentioning

confidence: 99%

Evolution from univariate to multivariate optimization methods in liquid chromatography

Lukulay

McGuffin

1996

J. Micro. Sep.

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Influence of organic modifiers on the rentention behaviour in reversed-phase liquid chromatography and its consequences for gradient elution

Cited by 393 publications

References 10 publications

Experimental hydrophobicity parameters of perfluorinated alkylated substances from reversed-phase high-performance liquid chromatography

Experimental hydrophobicity parameters of perfluorinated alkylated substances from reversed-phase high-performance liquid chromatography

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Evolution from univariate to multivariate optimization methods in liquid chromatography

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