Influence of Ovine Prolactin on Transport of Fluid and Sodium Chloride by the Mammalian Intestine and Gall Bladder

Mainoya, J. R.; Bern, Howard A.; Regan, John W.

doi:10.1677/joe.0.0630311

Cited by 34 publications

(17 citation statements)

References 23 publications

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“…Both PRL actions and signaling pathway obtained from the mouse colon seemed to be opposite to our findings; this indicated the species difference with regard to the types of PRL receptor, the signaling pathways, including the membrane proteins responsible for PRL activation. Although PRL has previously been reported to regulate electrolyte transport in the mammalian small intestine (7,18), the present results showed a direct effect of PRL on the colonic epithelium and confirmed the important role of PRL in maintenance of ionic homeostasis, especially during lactation and pregnancy.…”

Section: Discussionsupporting

confidence: 84%

Site-specific regulation of ion transport by prolactin in rat colon epithelium

Deachapunya

Poonyachoti

Krishnamra

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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The effect of prolactin (PRL) on ion transport across the rat colon epithelium was investigated using Ussing chamber technique. PRL (1 μg/ml) induced a sustained decrease in short-circuit current (I(sc)) in the distal colon with an EC(50) value of 100 ng/ml and increased I(sc) in the proximal colon with an EC(50) value of 49 ng/ml. In the distal colon, the PRL-induced decrease in I(sc) was not affected by Na(+) channel blocker amiloride or Cl(-) channel blockers, NPPB, DPC, or DIDS, added mucosally. However, the response was inhibited by mucosal application of K(+) channel blockers glibenclamide, quinidine, and chromanol 293B, whereas other K(+) channel blockers, Ba(2+), tetraethylammonium, clotrimazole, and apamin, failed to have effects. The PRL-induced decrease in I(sc) was also inhibited by Na(+)-K(+)-2Cl(-) transporter inhibitor bumetanide, Ba(2+), and chromanol 293B applied serosally. In the transverse and proximal colon, the PRL-induced increase in I(sc) was suppressed by DPC, glibenclamide, and bumetanide, but not by NPPB, DIDS, or amiloride. The PRL-induced changes in I(sc) in both distal and proximal colon were abolished by JAK2 inhibitor AG490, but not BAPTA-AM, the Ca(2+) chelating agent, or phosphatidylinositol 3-kinase inhibitor wortmannin. These results suggest a segment-specific effect of PRL in rat colon, by activation of K(+) secretion in the distal colon and activation of Cl(-) secretion in the transverse and proximal colon. Both PRL actions are mediated by JAK-STAT-dependent pathway, but not phosphatidylinositol 3-kinase pathway or Ca(2+) mobilization. These findings suggest a role of PRL in the regulation of electrolyte transport in mammalian colon.

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Section: Discussionsupporting

confidence: 84%

Site-specific regulation of ion transport by prolactin in rat colon epithelium

Deachapunya

Poonyachoti

Krishnamra

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…-activated conductances in mouse colon Regulation of electrolyte transport in the mammalian intestine by PRL has been reported in previous studies [28,29]. While these studies examined both acute and chronic effects of PRL, we focused in the present report on acute effects of PRL on electrolyte transport.…”

Section: +mentioning

confidence: 92%

Control of Ion Transport in Mouse Proximal and Distal Colon by Prolactin

Puntheeranurak

Schreiber

Spitzner

et al. 2007

Cell Physiol Biochem

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The lactogenic hormone prolactin (PRL) has been known to affect Ca²⁺ and electrolyte transport in the intestinal epithelium. In the present study we analyzed ion transport in mouse proximal and distal colon, and acute changes induced by PRL. In the proximal colon, carbachol activated a Ca²⁺ dependent Cl^- secretion that was sensitive to DIDS and NFA. In the distal colon, both ATP and carbachol activated K⁺ secretion. Ca²⁺ -activated KCl transport in proximal and distal colon was inhibited by PRL (200ng/ml), while amiloride sensitive Na⁺ absorption and cAMP induced Cl^- secretion remained unaffected. Luminal large conductance Ca²⁺ -activated K⁺ (BK) channels were largely responsible for Ca²⁺ -activated K⁺ secretion in the distal colon, and basolateral BK channels supported Ca²⁺ -activated Cl^- secretion in the proximal colon. Ca²⁺ chelating by BAPTA-AM attenuated effects of carbachol and abolished effects of PRL. Both inhibition of PI3 kinase with wortmannin and blockage of MAP kinases with SB 203580 or U 0126, interfered with the acute inhibitory effect of PRL on ion transport, while blocking of Jak/Stat kinases with AG 490 was without effects. PRL attenuated the increase in intracellular Ca²⁺ that was caused by stimulation of isolated colonic crypts with carbachol. Thus PRL inhibits Ca²⁺ dependent Cl^- and K⁺ secretion by interfering with intracellular Ca²⁺ signaling and probably by activating PI3 kinase and MAP kinase pathways.

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“…Treatment of rats with high (superphysiological) doses of ovine prolactin produced fluid shifts from intracellular to extracellular fluid compartments, of possible importance in maintaining vascular volume during acute stress [128]; a similar fluid shift from intracellular to extracellular spaces following ovine prolactin treatment, has been reported for the tilapia [111], As with other vertebrate classes discussed above, closer attention is being paid to transport across individual or gans. Intestinal sacs have been used for some time to de monstrate the stimulatory effect of prolactin on Na*, Cl and water absorption [117][118][119][120][121][122]169]. Other experiments de monstrate that prolactin also stimulates intestinal Ca2 transport [53,120] (and bone Ca-'* mobilization [150]); this may be one effect contributing to the hypercalcemic action mentioned above.…”

mentioning

confidence: 99%

Prolactin and Osmoregulation in Vertebrates

Loretz

Bern²

1982

Neuroendocrinology

173

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Studies on the roles of prolactin in vertebrate osmoregulation have expanded in recent years to include examination of ionic and osmotic factors contributing to the regulation of secretion by paraneuronal prolactin-secreting cells and the effects of prolactin on a number of ion- and water-transporting epithelial tissues. Coupled with information on whole-animal homeostatic effects, the application of surgical, tissue-culture, biophysical and biochemical techniques to individual components will facilitate broader and more integrated functional analyses of the important osmoregulatory effects of this hormone in all vertebrate classes.

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Influence of Ovine Prolactin on Transport of Fluid and Sodium Chloride by the Mammalian Intestine and Gall Bladder

Cited by 34 publications

References 23 publications

Site-specific regulation of ion transport by prolactin in rat colon epithelium

Site-specific regulation of ion transport by prolactin in rat colon epithelium

Control of Ion Transport in Mouse Proximal and Distal Colon by Prolactin

Prolactin and Osmoregulation in Vertebrates

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