Influence of pancreatic perfusates upon the carbohydrate metabolism of depancreatized animals

Abstract

Following the method of perfusion introduced by Clarkf, the effect of pancreatic perfusates upon the carbohydrate metabolism of entire animals was studied. The respiratory metabolism, the blood sugar, and the D:N ratio were studied in cats and dogs after depancreatization.Perfusates were made by perfusing aseptically and a t body teniperature the pancreases of cats, dogs, pigs, and oxen with Locke's solution. The first perfusates were made with an alkaline mediuni, later perfusates were made with Locke's solution modified by the substitution of llCl \-arying in strength from 0.1 per cent. to 0.7 per cent. for sodiuni bicarbonate.Respiratory quotients were obtained by a modified Jaquet method, the animal being confined in a respiration chamber through which a continuous stream of air was drawn for ventilation and from which a continuous sample of air was removed for analysis. In a later modification the stream of air from the respiration chamber n.as directed into a large gasometer for a twenty-minute period aiid the samples for aiialysis withdrawn from this total volume. Analyses were made by the Henderso:] and Bailey modification of Haldane's apparatus. The respiration chamber was frequently cliecked by analyses of outside air and by burning alcohol in it. D :N ratios and respiratory quotients typical for diabetes were obtained in the depancreatized animals.Following the administration of sugar in the form of destrose or siicrose by stomach tube pancreatic perfusates were given s u bcu t a n e o us1 y , i 11 t ra ve n o u s 1 y , a nd in t rape r it o nea 11 y . S i g iii fica n t increases were shown in the respiratory quotients and marked 1 Clark, Adlllont H., JOUT. Err. Med., 1916, sxiv., 621.