Influence of regeneration method and tissue source on the frequency of somatic variation in Populus to infection by Septoria musiva

Ostry, Michael E.; Hackett, Wesley P.; Michler, Charles H.; Serres, R.; McCown, Brent H.

doi:10.1016/0168-9452(94)90058-2

Cited by 38 publications

(17 citation statements)

References 19 publications

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“…Similar results were reported using the RAPD technique in Norway spruce (Fourré et al 1997), Japanese black pine (Goto et al 1998), Japanese white oak (Thakur et al 1999), loblolly pine (Tang 2001), almond (Martins et al 2004), hazelnut (Nas et al 2004, pedunculate oak (Valladares et al 2006), cork oak (Fernandes et al 2011), apricot (Soliman 2012), annatto (Siril and Joseph 2013), and seedless lemon (Goswami et al 2013). In contrast, RAPD analysis reported genetic variation in poplar (Ostry et al 1994;Rani et al 1995), white spruce (Isabel et al 1995(Isabel et al , 1996, peach (Hashmi et al 1997), teak (Gangopadhyay et al 2003), Idaho locust (Ngezahayo et al 2006), stone pine (Cuesta et al 2010), and olive (Leva et al 2012).…”

Section: Discussionsupporting

confidence: 68%

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Hosoi

Kuramoto

Maruyama

2015

Plant Biotechnology

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In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.

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Section: Discussionsupporting

confidence: 68%

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Hosoi

Kuramoto

Maruyama

2015

Plant Biotechnology

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“…Ostray et al 1994). However, the possibility of generating somaclonal variations still exists Raina 2000, Bindiya andKanwar 2003).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 94%

“…1). Micropropagated plants derived from shoot tips and axillary buds have been previously reported to maintain clonal stability (Ahuja 1987, Ostray et al 1994. However, occasionally there is still a chance of obtaining somaclonal variation through adopting this approach (Rani and Raina 2000).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers

Samantaray¹,

Maiti²

2010

Biologia plant.

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Rapid micropropagation was achieved in Chlorophytum borivilianum Santapau and Fernandes using shoot base as explants. Multiple shoots were induced on Murashige and Skoog's (MS) medium supplemented with 3.0 mg dm -3 6-benzylaminopurine, 0.1 mg dm -3 1-naphthaleneacetic acid, 150 mg dm -3 adenine sulphates and 3 % saccharose. Rooting was readily achieved upon transferring the shoots onto half strength MS medium supplemented with 0.1 mg dm -3 indolebutyric acid and 2 % saccharose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of the micropropagated plants. Thirty one arbitrary decamers were used to amplify genomic DNA from in vitro and in vivo plant material to assess the genetic stability. All RAPD profile analysis from micropropagated plants was genetically similar to mother plants.Additional key words: genetic stability, in vitro, medicinal plant, polymerase chain reaction.

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“…Leela et al (2011) using RAPD analysis also recorded no somaclonal variation of regenerants. In accordance with some authors, the use of pre-existing meristems such as shoot tips and axillary buds from the hardwood shoot cuttings lower the risk of somaclonal variations (Ahuja, 1987;Ostry et al, 1994;Wang and Charles, 1991). These kinds of competent and pre-determined explants have less necessity of high growth regulator concentrations for micropropagation process when compared with organogenesis or somatic embryogenesis process using no meristematic explants.…”

Section: Foliar Explants Organogenesismentioning

confidence: 80%

Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry

Franco¹,

Marques²,

Siqueira³

et al. 2014

Afr. J. Biotechnol.

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Influence of regeneration method and tissue source on the frequency of somatic variation in Populus to infection by Septoria musiva

Cited by 38 publications

References 19 publications

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers

Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry

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