Influence of sterilization processes on poly(ε-caprolactone) nanospheres

Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material

“…The observation that gamma-irradiation cross-links the polymer chains is consistent with irradiated PCL having a higher tensile strength (Table 4). It is also consistent with the results of Masson et al (1997) who showed that gamma irradiation (at 2.5 Mrad) increased M w in PCL nanospheres; they attributed this observation to cross-linking of polymer chains [8]. Narkis et al (1984) showed that gamma irradiation, at a much higher dose (26 Mrad) than required for sterilisation led to chain scission and cross-linking [7].…”

“…Narkis et al (1985) found that gamma irradiation induced chain scission and cross-linking [7], but their work focussed mainly on much higher doses than that required for sterilisation. Masson et al (1997) used the standard sterilisation dose of 25 kGy but were concerned only with PCL nanospheres [8].…”

A Practical Approach to the use of Nanoparticles for Vaccine Delivery

“…33,34 The initial nanoparticle preparation does not need to be aseptic if the nanoparticles are sterile filtered, also simplifying the process compared to microparticles. There are only a few reported literature cases of that take advantage of the sterile filtration potential of nanoparticles with one example was with poly(e-caprolactone) 35 and another example with PLG. 36,37 One reason for this is that the size needed for sterile filtration should be significantly less than 200 nm and many nanoparticles described in the literature are larger than this cutoff limit.…”

In vitro cytotoxicity testing of AB‐polymer networks based on oligo(ϵ‐caprolactone) segments after different sterilization techniques

“…13 To the authors' knowledge little is known about the influence of EO sterilization on poly (⑀-caprolactone) networks. 14 An increase of cell lysis was found after EO sterilization and following incubation of the polymer samples in MEM for 24 h and 1 week (Figure 2, Table I). The reason for this increase in cell lysis is not known.…”