Influence of Temperature on Mycelial Growth of some Pleurotus eryngii and Lentinula edodes Strains in vitro

Zăgrean, A. V.; Neaţă, G.; Stănciulescu, Bogdan

doi:10.15835/buasvmcn-hort:12353

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…This is probably due to the protein contents of the potato tuber, which is a suitable nutritional source. Further, mince potato, is commonly used to produce shiitake biomass under submerged culture (Aminuddin et al, 2007) and in petri dishes (Mahamud and Ohmasa, 2008), in the form of potato dextrose agar (PDA), the most used media for edible mushrooms (Zagrean et al, 2017). For shiitake, potato acts mainly as a carbon source, due to its high starch content, but also it contains 2.4% of protein (Loyola et al, 2010).…”

Section: Biomass Yields By Carbon Sourcesmentioning

confidence: 99%

Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

DurÃ¡n-Rivera¹,

Moreno-SuÃ¡rez²,

Rodas³

et al. 2018

Afr. J. Food Sci.

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In this study, the effect of three carbon sources (mannitol, minced potato and sucrose), two immobilization substrates (alginate and wood cylinders), and three surfactants (Tween 20, Tween 40 and Tween 80), were evaluated on eritadenine production using shiitake (Lentinula edodes) mycelium under submerged cultivation, in shake flasks within 20 days. Eritadenine and biomass were measured by HPLC and gravimetrically, respectively. Alginate immobilization of mycelium promoted significant enhancement of eritadenine yields of 88 mg/L, compared to the control (8.7 mg/L) and wood immobilization (14.8 mg/L). Likewise, eritadenine yields (72.4 mg/L) were enhanced by adding surfactant tween 20 to the broths in 0.5%, than control (8.7 mg/L) without surfactant. Tween 40 and 80 did not improve eritadenine yields, but both produced better biomass values (superior to 5 g/L) than the control (3.9 g/L). All carbon sources (sucrose, mannitol, mince potato, and glucose as control) produced similar low eritadenine yields, with best results (10.2 mg/L) by sucrose, although glucose produced the best biomass yields of 3.9 g/L. Also, carbon sources and the best biomass values did not show significant effect on eritadenine production. pH values in the best eritadenine yielding fermentations went down from 6 to 3-4, but pH had a low correlation with eritadenine yields. Finally, all data obtained in the present study are useful for optimizing culture conditions, towards industrialization of this important health improver metabolite (eritadenine).

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Section: Biomass Yields By Carbon Sourcesmentioning

confidence: 99%

Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

DurÃ¡n-Rivera¹,

Moreno-SuÃ¡rez²,

Rodas³

et al. 2018

Afr. J. Food Sci.

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Optimization of Technical Parameters on the Medium Formulation and Culture Conditions in Production of Pleurotus eryngii Liquid Spawn

Jong¹,

MyongIl²,

Ju³

et al. 2021

IJSRST

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Liquid spawn, an important technical support for industrial production of edible fungi, has some advantages, such as fast growth, strong activity, uniform quality and easy inoculation. However，the study of the fermentation parameters of Pleurotus eryngii liquid spawn is less, which cannot effectively guide the production practice. Therefore, in order to provide a theoretical basis for the production of liquid spawn and industrial production of Pleurotus eryngii, response surface analysis was used to optimize the liquid spawn formula and conditions. The best carbon and nitrogen source of liquid medium was glucose and yeast extract, respectively. The optimum formula of medium was obtained by response surface analysis. Combined with the practical operation, the formula was modified to 29.00 g/L glucose, 2.90g/L yeast extract, 0.90g/L KH2PO4 and 1.00g/L MgSO4. The effects of various factors on the dry weight of mycelia were analyzed, the results indicated that the influence follow the sequence: glucose, KH2PO4, yeast extract and MgSO4, among them, the first two factors had significant effect. The interaction between glucose and KH2PO4 had obviously effects on the dry weight of mycelia. The interaction between glucose and yeast extract had a certain effect. The optimum culture conditions of liquid spawn were obtained by response surface analysis. Combined with the actual situation, the conditions were modified to liquid volume 106.00mL/250mL, rotating speed 165.00r/min, temperature 23.60℃, initial pH 6.70. The effects of various factors on the dry weight of mycelia was analyzed, the results indicated that the influence follow the sequence: initial pH, liquid volume, temperature and rotating speed, among them, the first three factors had extremely significant effect. The interaction of liquid volume and rotating speed, temperature and initial pH had obviously effects on mycelial dry weight.

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Influence of Temperature on Mycelial Growth of some Pleurotus eryngii and Lentinula edodes Strains in vitro

Cited by 2 publications

References 2 publications

Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

Optimization of Technical Parameters on the Medium Formulation and Culture Conditions in Production of Pleurotus eryngii Liquid Spawn

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