Influence of the dietary inclusion of Gracilaria cornea and Ulva rigida on the biodiversity of the intestinal microbiota of Sparus aurata juveniles

Rico, Rosa María; Tejedor-Junco, María Teresa; Tapia‐Paniagua, Silvana; Alarcón, Francisco Javier; Mancera, Juan Miguel; Figueroa, Félix L.; Balebona, M.C.; Abdala-Díaz, Roberto; Moriñigo, Miguel A.

doi:10.1007/s10499-015-9964-x

Cited by 25 publications

(37 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…piscicida in S. senegalensis GI microbiota (Tapia-Paniagua et al, 2014a,b). Similarly, Rico et al (2016) detected predominant bands related to Photobacterium OTUs in DGGE gels when analyzing the GI microbiota of Sparus aurata fed diets containing Ulva rigida (5%, 15 and 25%). Tapia-Paniagua et al (2014a,b) and Rico et al (2016) suggested that the high abundance of naturally antagonistic species in the system may avoid the reach of infective concentrations and would contribute to the control of pathogen populations.…”

Section: Discussionmentioning

confidence: 81%

Modulation of Intestinal Microbiota in Solea senegalensis Fed Low Dietary Level of Ulva ohnoi

et al. 2019

View full text Add to dashboard Cite

Gastrointestinal (GI) microbiota has a relevant role in animal nutrition, modulation of the immune system and protection against pathogen invasion. Interest in algae as source of nutrients and functional ingredients for aquafeeds is increasing in order to substitute conventional feedstuffs by more sustainable resources. The diet is an important factor in the modulation of the microbiota composition, and functional ingredients have been proposed to shape the microbiota and contribute benefits to the host. However, fish microbiome research is still limited compared to other hosts. Solea senegalensis is a flat fish with high potential for aquaculture in South Europe. In this study, a characterization of the microbiome of S. senegalensis (GI) tract and the effects of feeding Ulva ohnoi supplemented diet has been carried out. Differences in the composition of the microbiota of anterior and posterior sections of S. senegalensis GI tract have been observed, Pseudomonas being more abundant in the anterior sections and Mycoplasmataceae the dominant taxa in the posterior GI tract sections. In addition, modulation of the GI microbiota of juvenile Senegalese sole fed for 45 days a diet containing low percentage of U. ohnoi has been observed in the present study. Microbiota of the anterior regions of the intestinal tract was mainly modulated, with higher abundance of Vibrio spp. in the GI tract of fish fed dietary U. ohnoi.

show abstract

Section: Discussionmentioning

confidence: 81%

Modulation of Intestinal Microbiota in Solea senegalensis Fed Low Dietary Level of Ulva ohnoi

et al. 2019

View full text Add to dashboard Cite

show abstract

“…have been even found to be beneficial as a member of the fish intestinal microbiota by its ability to aid with digestion of compounds, such as chitin ( 86 ), to produce polyunsaturated fatty acids or even antibacterial secondary metabolites that could inhibit the growth of other pathogenic bacteria ( 87 ). This genus has been reported as a member of the intestinal microbiota of marine farmed fish, including gilthead seabream ( 54 , 85 ), and it has been demonstrated that this genus is one of the most modulated genera in the fish when applying functional diets ( 88 ). Regarding the antimicrobial effect of the EOs-supplemented diet, an in vitro study demonstrated that the ethanolic extracts of oregano leaves, predominantly composed of carvacrol and thymol, presented a strong bactericidal activity against several pathogens including Photobacterium damselae , besides its immunostimulatory effect on gilthead seabream head kidney leukocytes ( 89 ).…”

Section: Discussionmentioning

confidence: 99%

Diet, Immunity, and Microbiota Interactions: An Integrative Analysis of the Intestine Transcriptional Response and Microbiota Modulation in Gilthead Seabream (Sparus aurata) Fed an Essential Oils-Based Functional Diet

et al. 2021

View full text Add to dashboard Cite

Essential oils (EOs) are promising alternatives to chemotherapeutics in animal production due to their immunostimulant, antimicrobial, and antioxidant properties, without associated environmental or hazardous side effects. In the present study, the modulation of the transcriptional immune response (microarray analysis) and microbiota [16S Ribosomal RNA (rRNA) sequencing] in the intestine of the euryhaline fish gilthead seabream (Sparus aurata) fed a dietary supplementation of garlic, carvacrol, and thymol EOs was evaluated. The transcriptomic functional analysis showed the regulation of genes related to processes of proteolysis and inflammatory modulation, immunity, transport and secretion, response to cyclic compounds, symbiosis, and RNA metabolism in fish fed the EOs-supplemented diet. Particularly, the activation of leukocytes, such as acidophilic granulocytes, was suggested to be the primary actors of the innate immune response promoted by the tested functional feed additive in the gut. Fish growth performance and gut microbiota alpha diversity indices were not affected, while dietary EOs promoted alterations in bacterial abundances in terms of phylum, class, and genus. Subtle, but significant alterations in microbiota composition, such as the decrease in Bacteroidia and Clostridia classes, were suggested to participate in the modulation of the intestine transcriptional immune profile observed in fish fed the EOs diet. Moreover, regarding microbiota functionality, increased bacterial sequences associated with glutathione and lipid metabolisms, among others, detected in fish fed the EOs supported the metabolic alterations suggested to potentially affect the observed immune-related transcriptional response. The overall results indicated that the tested dietary EOs may promote intestinal local immunity through the impact of the EOs on the host-microbial co-metabolism and consequent regulation of significant biological processes, evidencing the crosstalk between gut and microbiota in the inflammatory regulation upon administration of immunostimulant feed additives.

show abstract

“…Butyrate supplementation reversed the decrease of Vibrio only to some extent, following a restoration trend also observed in the intestinal mucus proteome. Similarly, diets with partial substitution of FM with seaweed ingredients induced a significant reduction of Vibrio species in the intestines of GSB juveniles [ 66 ]. Total replacement of FM by vegetable ingredients in GSB diets produced an increase of the genus Photobacterium in the mixed gut microbiota [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

et al. 2017

View full text Add to dashboard Cite

BackgroundThe constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative and more sustainable raw aquafeed materials, and plant ingredients have been used to replace marine feedstuffs in many farmed fish. The objective of the present study was to assess whether plant-based diets can induce changes in the intestinal mucus proteome, gut autochthonous microbiota and disease susceptibility of fish, and whether these changes could be reversed by the addition of sodium butyrate to the diets. Three different trials were performed using the teleostean gilthead sea bream (Sparus aurata) as model. In a first preliminary short-term trial, fish were fed with the additive (0.8%) supplementing a basal diet with low vegetable inclusion (D1) and then challenged with a bacteria to detect possible effects on survival. In a second trial, fish were fed with diets with greater vegetable inclusion levels (D2, D3) and the long-term effect of sodium butyrate at a lower dose (0.4%) added to D3 (D4 diet) was tested on the intestinal proteome and microbiome. In a third trial, the long-term effectiveness of sodium butyrate (D4) to prevent disease outcome after an intestinal parasite (Enteromyxum leei) challenge was tested.ResultsThe results showed that opposed forces were driven by dietary plant ingredients and sodium butyrate supplementation in fish diet. On the one hand, vegetable diets induced high parasite infection levels that provoked drops in growth performance, decreased intestinal microbiota diversity, induced the dominance of the Photobacterium genus, as well as altered the gut mucosal proteome suggesting detrimental effects on intestinal function. On the other hand, butyrate addition slightly decreased cumulative mortality after bacterial challenge, avoided growth retardation in parasitized fish, increased intestinal microbiota diversity with a higher representation of butyrate-producing bacteria and reversed most vegetable diet-induced changes in the gut proteome.ConclusionsThis integrative work gives insights on the pleiotropic effects of a dietary additive on the restoration of intestinal homeostasis and disease resilience, using a multifaceted approach.Electronic supplementary materialThe online version of this article (10.1186/s40168-017-0390-3) contains supplementary material, which is available to authorized users.

show abstract

Influence of the dietary inclusion of Gracilaria cornea and Ulva rigida on the biodiversity of the intestinal microbiota of Sparus aurata juveniles

Cited by 25 publications

References 66 publications

Modulation of Intestinal Microbiota in Solea senegalensis Fed Low Dietary Level of Ulva ohnoi

Modulation of Intestinal Microbiota in Solea senegalensis Fed Low Dietary Level of Ulva ohnoi

Diet, Immunity, and Microbiota Interactions: An Integrative Analysis of the Intestine Transcriptional Response and Microbiota Modulation in Gilthead Seabream (Sparus aurata) Fed an Essential Oils-Based Functional Diet

Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets

Contact Info

Product

Resources

About