Influence of the G2m(n) allotype and age on IgG subclass distribution in antibodies to dietary proteins in children with coeliac disease

Abstract

SUMMARYIn 47 children with coeliac disease, IgG 1 was the dominant IgG subclass (mean 61 %) in antibodies to gliadin, a protein component of wheat; IgG2 (22%) and IgG3 (15%) were also found. Very little IgG4 (3%) was detected. Both G2m(n)allotype and the age ofthe patient had an independent effect on the subclass distribution. The effects of these two factors seem similar to those described earlier on responses to polysaccharides; both G2m(n) and age increase the expression of IgG2 antibodies and concomitantly the share of IgGI is deereased. The average increase in the share of IgG2 brought about by n/n-genotype was 25 3% (95% eonfidence limits 12 2-38 4%; /'<0 001) and that brought about by each additional year of age ofthe patient 11% (0 3-19%; ^=0 006). IgG antibodies tô -lactoglobulin and ovalbumin consisted mainly of IgGI and IgG4. The titres ofthe IgG subclasses were low and in many cases below detection level. No association with G2m(n) genotype and share of IgGI or IgG4 could be demonstrated. Age was inversely correlated with the shares of IgGI in antibodies to ^-lactoglobulin and ovalbumin.