Influence of type and proportion of lyoprotectants on lyophilized ginsenoside Rg3 liposomes

Li, Jianying; Hu, Meina; Xu, Huan; Xiu, Yu; Ye, Feifei; Wang, Kaiqian; Luan, Xiaojiao; Li, Ling; Zhang, Di

doi:10.1111/jphp.12489

Cited by 22 publications

(15 citation statements)

References 27 publications

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“…Freeze-drying is a promising method to extend the life shelf of liposomes; however, the sublimation of ice or crystallization may occur during the phase transition, which can disrupt the integrity of lipid membranes. Therefore, the addition of lyoprotectants is necessary to prevent changes in appearance, increases in particle size, and decreases in drug loading [29,30] . Different lyoprotectants were screened in this study according to the appearance, re-dispersity, particle size, and entrapment efficiency of lyophilization of G-Rg3-PLP (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Preparation of PEGylated Liposomal Ginsenoside;Formulation Design and in vitro Evaluation

Cui¹,

Yang²,

Sun³

et al. 2020

ijps

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Cui et al.: Ginsenoside Rg3-loaded PEGylated liposomesIn this study, ginsenoside Rg3-loaded PEGylated liposomes were prepared and optimized using the Box-Behnken design. These liposomes were characterized, the cumulative release profiles were investigated and compared with ginsenoside Rg 3 -loaded liposomes in vitro. To improve the stability ginsenoside Rg3-loaded PEGylated liposomes were freeze-dried and the lyoprotectants to be added were screened. The results showed that the liposomes have a small particle size (152.58±0.74 nm) and spherical shape. The encapsulation efficiency and drug-loading rate were approximately 85.24±1.02 and 7.44±0.08 %, respectively. For lyoprotectants, 2 % lactose was chosen as the lyophilized protectant according to the appearance, re-dispersity, particle size, and entrapment efficiency of lyophilization of ginsenoside Rg3loaded PEGylated liposomes. In vitro release showed that ginsenoside Rg3-loaded PEGylated liposomes showed a more obvious sustained release effect, which suggests that ginsenoside Rg3-loaded PEGylated liposomes might enhance the therapeutic effect of ginsenoside Rg3.

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Section: Resultsmentioning

confidence: 99%

Preparation of PEGylated Liposomal Ginsenoside;Formulation Design and in vitro Evaluation

Cui¹,

Yang²,

Sun³

et al. 2020

ijps

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“…This is a good indication that the employed freezedrying process was successfully produced an amorphous solid. Amorphous system could protect the liposomes in the formula since crystal components could damage the integrity of bi-layer membrane, 12 and can be detrimental to the structure of labile bio-molecules such as proteins. 25 Figure 2.…”

Section: X-ray Diffraction Analysismentioning

confidence: 99%

“…7,8 Saccharides such as glucose, lactose, maltodextrins, and mannitol are commonly used as lyoprotectant for protein and liposomes in their dry products. [9][10][11][12][13] Among them, maltodextrins-oligomer of glucose-draw attention to its amorphous nature which became an advantage in preserving protein structure during freeze-drying and maintain its performance after the process. 14 Another compound which also extensively studied as lyoprotectant is mannitol.…”

Section: Introductionmentioning

confidence: 99%

Physical Characteristics of Liposomal Formulation Dispersed in HPMC Matrix and Freeze-Dried Using Maltodextrin and Mannitol as Lyoprotectant

Nugraheni¹,

Setyawan²,

Yusuf³

2017

Pharm Sci

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Background: The present study aims to design formulation of liposomes that are well-preserved during freeze-drying. The combination of Hydroxy Propyl Methyl Cellulose (HPMC) as dispersion matrix and lyoprotectants; maltodextrin or mannitol, was employed to prevent aggregation and/or recrystallization. The obtained dry products were investigated in terms of their physical characteristics. Methods: Liposomes were prepared using thin film method and hydrated with the lyoprotectant solution. The formed liposomes were mixed with HPMC gel and freeze-dried. The obtained solid products were characterized using Differential Scanning Calorimetry (DSC), X-Ray Diffraction (XRD), and Scanning Electron Microscopy (SEM). Results: The DSC thermograms of formulations with maltodextrin were relatively homogenous, yet exhibiting meta-stable properties. In contrast, the formulations using mannitol showed phase separation. These results were confirmed by XRD data, in which formulations with maltodextrin showed no intensive peaks, indicating amorphous solid while the formulations with mannitol exhibited more intensive peaks, indicating the presence of crystalline solids. The SEM images of both maltodextrin and mannitol-containing formulations showed porous matrix with spherical liposomes trapped in the matrices. The SEM images also correspond to the DSC and XRD data, where crystalline solid existed in the mannitol-containing formula. Conclusion: The developed liposomes formulation using combination of HPMC matrix and maltodextrin showed potential in preserving liposomes structure, contrary to those of using mannitol.

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“…3. Comparing the retention time, UV spectra and mass spectra of the components in SFI with those of the reference standards, the peaks could be unambiguously identified as the known constituents, adenine (3) [17,18], 5-hydroxymethyl-furaldehyde (5) [19,20], calycosin-7-O-β-D-glucopyranoside (9) [21,22], lobetyolin (13) [11,23], ononin (14) [21,24], and astragaloside IV (3′) [25,26], respectively. The other components were tentatively identified according to their MS fragmentation pattern, and also by studying their UV spectral characteristics and reported literatures.…”

Section: Identification Of Constituents In Sfi In Fingerprint Chromatmentioning

confidence: 99%

“…Considering the complicated constituents with various spectral properties of SFI, a reliable, convenient, and generally applicable method for quality control of SFI is urgently required. The HPLC method coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) possesses the advantages of complementarities and cost-effectiveness, highly suited for routine analysis and quality evaluation [14,15]. On the other hand, using ultrafast liquid chromatography (UFLC) coupled with DAD and quadrupole time-of-flight (Q-TOF) tandem mass spectrometry (MS/MS) technique could simultaneously obtain ultraviolet (UV) absorption and mass spectrometric data of each individual compound within a short time, and then the components could be identified or tentatively characterized based on the retention time, UV spectra, fragmentation patterns, free chemical databases, and reported literatures.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

Wang

Liu

Tong

et al. 2015

Acta Chromatographica

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Summary. Shenqi Fuzheng Injection (SFI) is a traditional Chinese medicine injection, widely used to enhance immune function of clinical cancer patients undergoing chemotherapy. In this study, a high-performance liquid chromatography-diode array detection-evaporative light scattering detection (HPLC-DAD-ELSD) method was established for quality control of SFI, which could simultaneously semiquantitatively reflect the constituents displayed in the chromatographic profile of SFI. The relative retention time and relative peak areas of the 21 common peaks related to the reference peak were calculated. The validity and advantage of this method were validated by systematically comparing chromatograms of 10 batches of SFI samples with the analytical methods of principal component analysis and angle cosine method recommended by the State Food and Drug Administration of China. Moreover, a total of 21 constituents of SFI were identified or tentatively characterized in the fingerprint via ultrafast liquid chromatography-diode array detection-quadrupole time-of-flight (UFLC-DAD-Q-TOF) tandem mass spectrometry technique on the basis of the retention time, ultraviolet spectra, fragmentation patterns, and reported literatures. All the results proved that the technique was useful in comprehensive quality evaluation of SFI and further study.

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Influence of type and proportion of lyoprotectants on lyophilized ginsenoside Rg3 liposomes

Abstract: The application of glucose-mannitol composite lyoprotectants can obtain a good G-Rg3 lyophilized preparation.

Cited by 22 publications

References 27 publications

Preparation of PEGylated Liposomal Ginsenoside;Formulation Design and in vitro Evaluation

Preparation of PEGylated Liposomal Ginsenoside;Formulation Design and in vitro Evaluation

Physical Characteristics of Liposomal Formulation Dispersed in HPMC Matrix and Freeze-Dried Using Maltodextrin and Mannitol as Lyoprotectant

Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

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