Influence of ZnO Nanoparticles on Candida albicans of Human Male Pleural Fluid

Abstract

The utilization of metal oxide nanoparticles, especially zinc oxide, is of a great importance in the medical field because of its physical and chemical properties as well as its antimicrobial potential effects. In our study, the ZnO nanoparticles were synthesized by the precipitation method where pH=14. ZnO nanoparticles were characterized by ultraviolet-visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and atomic force microscope (AFM). Antifungal activity of the ZnO was tested against candida albicans. The results showed that C. albicans (15 samples) became resistant to the fungal activity after testing its sensitivity to several types of fungal antibiotics. UV-vis spectroscopy, XRD, TEM and AFM showed that this precipitation synthetic method can produce a good quality of ZnO nanoparticles with sizes in the range of nanometer scale. In ZnO NPs (calcined), the inhibition zone diameter of C. albicans was 11 mm at a ZnO NPs concentration of 800 mg/ml, while no inhibition zones were formed at the other concentrations (0.25, 0.5, 1, 2, 10, 50 and 500 mg/ml). Using ZnO NPs (not calcined), the inhibition zone diameter of C. albicans was 24 mm at a concentration of 800 mg/ml, while no inhibition zones were observed at the other concentrations (0.25, 0.5, 1, 2, 10, 50 and 500 mg/ml). In addition, a toxicity test was performed on mice and proved that ZnO NPs are effective against C. albicans with a toxic effect on liver and spleen cells in rats. The aim of this research was to characterize the in vitro activity of ZnO nanoparticles synthesized by the precipitation method against C. albicans of human male pleural fluid using the well diffusion method as well as their toxic effects on both liver and spleen cells in mice.

Evaluation of Antibacterial and Cytotoxicity Properties of Zinc Oxide Nanoparticles Synthesized by Precipitation Method against Methicillin-resistant Staphylococcus aureus

“…This result almost agrees with other studies 12 synthesis shape crystallite size that found the average ZnO NPs. Rod-like shape of ZnO-NPs by the SEM image (Figure 4).…”

Enhanced Antifungal Activity of WS2/ZnO Nanohybrid against Candida albicans

“… The antimicrobial activity of some nanomaterials against C. albicans as evaluated by agar diffusion assay in terms of zone diameter were Fluconazole Ag, 26 mm at 20 μL per disk; ZnO, 11 mm at 0.8 μg/mL; ZnO, 11.4 mm at 0.020 μg/mL; and WS 2 /ZnO nanohybrid, 6 mm at 6000 μg/mL.…”

Synthesis of Copper Oxide Nanostructures by Ar Plasma Jet and Study of Their Structural and Optical Properties

“…There were no secondary peaks in the XRD pattern, indicating that the formed nanoparticles were pure. The crystallite size of the CuO NPs was calculated depending on Debye-Scherrer Equation (3) [15,16]:…”