Influences of Surfactant Tween 80 on the Gas Production, Cellulose Digestion and Enzyme Activities by Mixed Rumen Microorganisms

Abstract

The surfactant Tween 80 was evaluated for its ability to influence cumulative gas production, cellulose digestion, and enzyme activities by mixed ruminal microorganisms grown on barley grain or Orchardgrass hay. The addition of Tween 80 at a level of 0.10% significantly (p<0.05) decreased the cumulative gas production rate from both barley grain or Orchardgrass hay substrates. However, 0.05% Tween 80 did not affect gas production rates compared to the control treatment. The addition of 0.05% Tween 80 to cultures growing on barley grain resulted in a significant increase in cellulase (90.01%), xylanase (90.73%) and amylase (487.25%) activities after 30 h incubation. Cultures utilizing Orchardgrass hay had a significant increase in cellulase (124.43%), xylanase (108.86%) and amylase (271.22%) activities after 72 h incubation. These increases in activities were also observed with cultures supplemented with 0.10% Tween 80 throughout all the incubation times tested. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some of key enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our data indicates potential uses of the surfactant Tween 80 as a feed additive for ruminant animals.

Pretreatment of surfactant Tween 80 and fibrolytic enzyme influencing volatile fatty acid and methane production of a total mixed ration by mixed rumen microorganisms at in vitro

“…2000; Goto et al . 2003a,b; Lee and Ha 2003; Wang et al . 2003), suggesting a use of surfactants in the feed industry and farming.…”

Degradability and gas production of diets enriched with additives in cattle or sheep inoculum

“…For the same product, there was a decrease in the cumulative gas production from barley grains (Hordeum vulgare) after 36 h of incubation, when added at the dose of 0.10%. In the proportion of 0.05%, there was no difference in relation to control (Lee & Ha, 2003). However, in the same study, no difference in gas production was observed after 96 h of incubation for orchard grass hay (Dactylis glomerata L.).…”

Tween 80-induced esterase production by Trichoderma harzianum in submerged fermentation: An esterase activity assay using α-naphthyl acetate as substrate

“…Tween 80 is a surfactant that has been used for several purposes in microbial cultures. This surfactant has been used to enhance the enzymatic hydrolysis of cellulolytic material in the industry (Jin et al, 2016), in agricultural applications (Wang et al, 2003), to decrease the surface and interfacial tensions for increasing substrate bioavailability in bioremediation studies (Uzoigwe et al, 2015;Wijaya et al, 2016;Yang et al, 2021) among other applications (Lee & Ha, 2003). Akpinar & Ozturk (2017) have suggested that Tween 80 enhances permeability of the cell membrane, which facilitate enzyme production and it can also protect the enzyme structure and its activity.…”