Ingestion of bonito extract ameliorates peripheral blood flow in mice loaded from over crowding stress

Abstract

Bonito extract (BE) has been shown to improve various fatigue-related symptoms. The possibility that the improvement of blood flow contributes to the improvement of fatigue-related symptoms has been reported. However, even though BE has been found to increase peripheral blood flow in humans, an understanding of its mechanisms has remained elusive. The purpose of the present study is to construct an animal model system with which the blood flow-increasing effects of BE can be examined. Using mice loaded with crowding stress, an attempt was made to reproduce the increases in peripheral blood flow observed in humans after a single administration of BE. In this study, the crowded-condition mice (20 mice/cage) showed significantly increased catecholamine levels (noradrenaline, adrenaline, and dopamine) in their circulating blood and a decreased rate of skin blood perfusion in comparison with the normal-condition mice (6 mice/cage). The rate of skin blood perfusion was significantly increased by BE in the crowded-condition mice in comparison with the controls, but not influenced by BE in the normal-condition mice. This suggests that BE expands the vascular diameter by affecting the constriction of vessels induced by catecholamines.Bonito extract (BE) is commonly used as soup stock in Japan. Bonito (skipjack tuna, Katsuwonus pelamis) is the name given to various species of medium-sized, predatory fish of the genus Sarda in the mackerel family and is known as katsuo in Japan. It is smoked and dried to make dried bonito, katsuobushi, which is an important ingredient in dashi (Japanese soup stock). Dried BE (katsuobushi-dashi) has a specific flavor and is used extensively in Japanese cuisine (7, 15) and traditional foods in Japan; for instance, in kachu-yu (a soup containing dried bonito and soy paste) in Okinawa and in cha-bushi (a soup containing dried bonito, soy paste, and green tea) in Kagoshima. Furthermore, it is consumed as a nutritional supplement that is believed to promote recovery from fatigue. We have previously demonstrated the anti-fatigue effects of BE in both animals and humans. Animal studies have shown that the administration of BE aids recovery from physical fatigue (11,18,19), and in humans the consumption of BE improves mental fatigue (13, 17), visual fatigue (8, 9), and shoulder stiffness (21). To examine the factors that contribute to the recovery from fatigue brought about by ingestion of BE, the effects of BE on the peripheral circulation were studied. Skin blood flow in humans is increased by the ingestion of BE, reaching a maximum 25-30 min after the ingestion (22). In another study, it became clear that the daily ingestion of BE for two weeks induces increases in skin blood flow at rest (20). Furthermore, the changes in blood flow that occurred after the ingestion of BE were significantly correlated with changes in fatigue scores on the profile of mood states (POMS) questionnaire. From these results, it is probable that the improvement of blood flow contributes to some degree to

Effective prevention of sorafenib-induced hand–foot syndrome by dried-bonito broth

“…Interestingly, in terms of its mechanism, it has been reported that the ingestion of bonito broth increases peripheral blood flow in humans, 9 resulting in an antioxidative effect and an improvement in mood. 9 , 12 In addition, an increase in the cerebral blood flow in mice 10 and rats 11 has been evidenced in the groups ingesting bonito broth, resulting in a decrease in stroke in hypertensive rats. 11 …”

<p>Effective prevention of sorafenib-related vascular damage induced adverse events and maintenance of hepatic function by dried bonito broth and histidine</p>

“…Vascular damage from SOR has also been reproduced in the vascular area dimension of GFP-labeled vessels in the transgenic medaka model 5. HIS, the major component among the amino acids contained in the bonito extract,11 alleviated this vascular damage in the medaka fin by maintaining the vascular area dimension without growing new vessels and exhibited no tumor cell growth 5. The results from an animal model and in vitro study have suggested that DBB and HIS may contribute to the maintenance of blood flow with no evidence of tumor growth.…”

Dietary Protein Derived from Dried Bonito Fish Improves Type‐2 Diabetes Mellitus‐Induced Bone Frailty in Goto‐Kakizaki Rats

“…Honda and others () suggested that ingestion of BF decreases oxidative stress in humans and that the BF may have antioxidative effects. Lukas and others () indicated that ALP activity and the level of thiobarbituric acid reactive substances were mutually decreased by dietary fish oils containing 0.2% DHA.…”

“…Bonito ( Katsuwonus pelamis ) is a medium‐sized, predatory fish of the genus Sarda , in the mackerel family. BF is used as one of the most traditional food ingredients in Japan, and many nutritional benefits have been reported (Matsumoto and others ; Nozawa and others ; Honda and others ). Our previous data have shown increases in bone mass and bone strength in growing Wistar rats fed with a BF diet compared with a casein diet (unpublished data).…”