Ingestion of ice-nucleating active bacteria increases the supercooling point of the lady beetle Hippodamia convergens

Strong‐Gunderson, Janet M.; Lee, Richard; Lee, Marcia R.; Tammy, J Riga

doi:10.1016/0022-1910(90)90116-w

Cited by 54 publications

(35 citation statements)

References 35 publications

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“…Some bacteria are extremely potent nucleating agents capable of catalyzing ice nucleation in this range (15). Recently, our laboratory demonstrated that ice nucleating bacteria have the potential to increase markedly the SCP of insects (24). It is possible that ice nucleating bacteria are present in the gut of Tensbrio and responsible for these extremely high SCPs.…”

Section: Discussionmentioning

confidence: 99%

Regulation of supercooling and nucleation in a freeze intolerant beetle (Tenebrio molitor)

Johnston

Lee

1990

Cryobiology

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Section: Discussionmentioning

confidence: 99%

Regulation of supercooling and nucleation in a freeze intolerant beetle (Tenebrio molitor)

Johnston

Lee

1990

Cryobiology

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“…In addition, INA bacteria have been used in artificial snowmaking (47), cloud seeding (15,39), bioinsecticides (34), frozen-food applications (2,44), and diagnostic applications (43). The molecular basis for the Ina' phenotype has been established through cloning and sequencing of ina genes and demonstration that a unique outer membrane protein with a highly repetitive amino acid sequence is responsible for the nucleation of freezing of water at relatively warm temperatures (reviewed in references 40 to 42).…”

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confidence: 99%

High-level expression of ice nuclei in a Pseudomonas syringae strain is induced by nutrient limitation and low temperature

1993

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Attempts were made to maximize the expression of ice nuclei in Pseudomonas syringae Ti isolated from a tomato leaf. Nutritional starvation for nitrogen, phosphorous, sulfur, or iron but not carbon at 320C, coupled to a shift to 14 to 18'C, led to the rapid induction of type 1 ice nuclei (i.e., ice nuclei active at temperatures warmer than -50C nuclei and the number of bacterial cells in a culture (i.e., ice nucleation frequency) varied with incubation temperature, growth medium composition, culture age, and genotype. Hirano et al. (9) showed that culturing of different Pseudomonas syringae strains under identical conditions results in ice nucleation frequencies at a given temperature, such as -50C, that vary from one in 100 cells to as few as one in 106 cells. The basis for this variability is unknown. Enhanced expression of ice nuclei, especially type 1 nuclei, has been noted for P. syringae cultures grown at 20 to 240C on nutrient agar containing glycerol (22); optimal conditions increased -50C ice nuclei from 1 in 410,000 to 1 in 72 cells. Using a chemically defined medium, Pooley and Brown (31) similarly noted enhanced ice nucleation frequencies and elevated threshold ice nucleation temperatures for cultures of P. syringae grown on solid versus liquid media. For submerged cultures of P. syringae, Pooley and Brown found that the highest threshold ice nucleation temperatures and frequencies were achieved in mid-to late-log-phase cultures grown in a chemically defined medium rather than a complex growth medium. They suggested that components within the complex medium may have inhibitory effects on the expression of ice nuclei, which is maximal late in the growth cycle. Programmed fermentations designed to achieve maximal cell densities and ice nucleation frequencies have taken advantage of this correlation between the temporal expression of ice nuclei in the cell cycle and nutritional growth requirements (18). In this work, maximal ice nucleation frequencies at -50C, approaching one active nucleus in 10 cells to one in every cell in the population, were achieved for submerged cultures of P. syringae. This result was accomplished by supplying stoichiometrically limiting quantities of nitrogen 4062 on May 9, 2018 by guest

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“…In a related coleopteran, Hippodamia convergens Guérin-Méneville (Coccinellidae), ice-nucleating active bacteria (i.e. Pseudomonas syrringae, Erminia herbicola) were correlated with a ~12 °C increase in the SCP (Strong-Gunderson et al 1990). Ice-nucleating bacteria have been found in field-collected insects (Lee et al 1987), but it is unknown what effects 31 d of artificially cold hardening may have had on gut bacteria abundance and diversity in X. glabratus.…”

Section: Resultsmentioning

confidence: 97%

Supercooling in the Redbay Ambrosia Beetle (Coleoptera: Curculionidae)

Formby¹,

Krishnan²,

Riggins³

2013

Florida Entomologist

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Ingestion of ice-nucleating active bacteria increases the supercooling point of the lady beetle Hippodamia convergens

Cited by 54 publications

References 35 publications

Regulation of supercooling and nucleation in a freeze intolerant beetle (Tenebrio molitor)

Regulation of supercooling and nucleation in a freeze intolerant beetle (Tenebrio molitor)

High-level expression of ice nuclei in a Pseudomonas syringae strain is induced by nutrient limitation and low temperature

Supercooling in the Redbay Ambrosia Beetle (Coleoptera: Curculionidae)

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