Inhibition activity of Î±-glucosidase by infusion  and ethanol extract of white tea and stevia combination

Trinoviani, Elvi; Kholisoh, Ai; Ar-Rifa, Nisa Fitriani; Rustamsyah, Ardi

doi:10.22302/pptk.jur.jptk.v19i2.113

Cited by 3 publications

(4 citation statements)

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“…The antioxidant activity test was carried out using the DPPH method to evaluate antioxidants using a spectrophotometer [34] with the working mechanism of the purple DPPH color reducing to light yellow due to antioxidants donating their hydrogen atoms to form stable DPPH radicals [35]. The α-amylase inhibitory test uses the DNS method, where alpha amylase hydrolyzes amylylum to maltose as a reducing sugar which reduces 3,5-dinitrosalicylic acid (brownish yellow) to 3-amino-5nitrosalicylic acid (reddish orange) and reducing sugar.…”

Section: Antioxidant Activity and Amylase Inhibitory 70% Ethanol Extr...mentioning

confidence: 99%

Phytochemical Properties, Antioxidant Activity and α- Amilase Inhibitory of Curcuma Caesia

Kartini

Juariah

Mardhiyani

et al. 2023

ARASET

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Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia, which produces free radicals, particularly reactive oxygen species (ROS), through glucose auto-oxidation and glycosylation. Administration of antioxidants and polyphenolic compound components can capture free radicals and reduce oxidative stress. Polyphenols are also able to reduce blood glucose levels by inhibiting the action of amylase enzymes as carbohydrate catabolism so that glucose absorption does not occur. One of the plants that contain polyphenols is Black Turmeric (Curcuma caesia). Some studies on the rhizomes of this plant include methanol extracts containing phenolics, Flavonoids, alkaloids, triterpenoids, saponins, and tannins. Dry powders and wet rhizome powders were studied to have antioxidant activity. Research on the inhibition of the enzyme alfa amylase is still lacking. This study aims to determine the content of chemical compounds using 70% ethanol solvent. The total phenolic and flavonoid assay with spectrophotometry, determine antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) at 490 nm and inhibition of α-amylase enzyme using 3,5- dinitro salicylic acid (DNS) at 529 nm. The results showed that phenolic levels were 235 mgGAE/100gr, flavonoids were 61 mg QE/100gr, Alkaloids 30 mg Quinine /100gr and saponin 158 mg Quillaja bark/100gr, dan tannin 535mg tannic acid/100gr. DPPH antioxidant activity obtained IC50 value of 299,18 µg/mLµg/mL and α- amylase inhibitory 50,26 µg/mLµg/mL. The research showed that 70% ethanol extract Curcuma caesia roxb contains phenolic, flavonoids, alkaloids, quinine, and saponins constituents and have been antioxidant activity and high α-amylase inhibitory

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Section: Antioxidant Activity and Amylase Inhibitory 70% Ethanol Extr...mentioning

confidence: 99%

Phytochemical Properties, Antioxidant Activity and α- Amilase Inhibitory of Curcuma Caesia

Kartini

Juariah

Mardhiyani

et al. 2023

ARASET

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“…Green tea is used to decrease obesity (Purwanto, Darmawati, Purwaningsih, & Ners, 2014;Rahmanisa & Wulandari, 2016;Sari, 2015), dia-Copyright ©2019 ISEKI-Food Association (IFA) 10. 7455/ijfs/8.2.2019.a5 Nomenclature STZ Streptozotocin NA Nicotinamide BW Body Weight GOD/PAP/POD Enzymatic colorimetric method betes (Bait, 2010;Cheng, Shen, & Wu, 2009;Efendi, Damayanthi, Kustiyah, & Kusumorini, 2010;Holidah, Yasmin, & Christianty, 2018;Ilma, 2016;Rohdiana, Firmansyah, Setiawati, & Yunita, 2012;Wibowo, Kusmana, Suryani, Hartati, & Oktadiyani, 2009;Yilmazer-Musa, Griffith, Michels, Schneider, & Frei, 2012); black tea to treat diabetes (Bait, 2010;Deswati & Nur Maryam, 2017;Holidah et al, 2018;Rosalia, Indrasari, Tangsilan, Jayadi, & Danu, 2017); white tea to treat diabetes (Trinoviani, Kholisoh, Ar-Rifa, & Rustamsyah, 2016) and kombucha green tea is used as a hepato protective agent. The role of tea in health is related to the presence of beneficial compounds such as polyphenol, theophylline, flavonoids, tannins, Vitamin C and E, catechins and some minerals (Majid & Nurkholis, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Considering the reported health benefits of tea people started brewing tea from leaves other than C. sinensis leaves, which is known as herbal tea. Some examples are, herbal tea from stevia leaves to treat diabetes (Trinoviani et al, 2016), mulberry leaves tea (Bait, 2010;Efendi et al, 2010), guava leaves herbal tea for diabetes (Cheng et al, 2009), stevia leaves tea for antioxidant (Ariviani & Ishartani, 2009), avocado leaves' tea for antioxidant, soursop leaves' black tea for uric acid treatment (Hardoko, Puspitasari, & Amalia, 2015), soursop leaves' extract to treat diabetes (Moniaga, 2014), soursop leaves' green tea to treat diabetes (Hardoko, 2015), fragrant pandan (Pandanus amaryllifolius Linn) leaves' water extract to treat diabetes (Prameswari & Widjanarko, 2014). The roles of plants or products from plants as natural medicines are related to their bioactive compounds, which vary with the type of the plants.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic activity of herbal green tea extract from white mangrove (Avicennia marina) leaves towards blood glucose level of diabetic wistar rats (Rattus novergicus)

Hardoko

Nafi’ah²,

Sasmito³

et al. 2019

IJFS

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Green tea Camellia sinensis contains polyphenol that has antidiabetic activity. Mangrove leaves also contain polyphenol which potentially gives these leave antidiabetic activity. The aim of this research was to determine the ability of herbal green tea extract from white mangrove (Avicennia marina) leaves to decrease blood glucose level of diabetic Wistar rats (Rattus novergicus). The method used was experimental and involved giving a herbal green tea extract from white mangrove leaves with concentration of 100, 200 and 300 mg/200g BW/day, and positive control, i.e. glybenclamide (0.09 mg/200 g BW/day), to diabetic rats injected with Streptozotocin (STZ) and Nicotinamide (NA). The rats were observed on day 0, 5, 10 and 15. The results showed that the herbal green tea extract from white mangrove leaves decreased the blood glucose level of diabetic rats. The effective extract dose that decreased the blood glucose level of diabetic rats was 300 mg/200 g BW, which is comparable to the effect produced by glybenclamide (antidiabetic medicine). This dose could decrease the blood glucose level of diabetic rats to reach a normal blood glucose level after 20 days.

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“…Pemberian ekstrak teh putih pada tikus dapat menurunkan kolesterol total, trigliserida, LDL serta meningkatkan HDL (Dahlia et al, 2017). Aktivitas lainnya teh putih menunjukkan penghambatan terhadap xanthine oksidase secara in vitro (Rustamsyah et al, 2016) dan juga seduhan teh putih yang dikombinasikan dengan daun stevia dapat menghambat enzim α-glucosidase secara in vitro (Trinoviani et al, 2016). Kandungan kimia teh putih yang memiliki banyak manfaat kesehatan adalah kelompok senyawa polifenol secara khusus adalah senyawa kelompok turunan katekin (Dias et al, 2013) Aktivitas farmakologi dan kajian senyawa teh putih telah banyak di bahas namun masih perlu dilakukan penelitian mengenai kandungan senyawa polifenol teh secara kuantitatif untuk the yang beredar dipasaran.…”

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Analisis Fenol dan Flavonoid Total Pada Beberapa Teh Putih (Camellia sinensis L.) yang Beredar di Pasaran

Rustamsyah¹,

Kartini²,

Martiani³

et al. 2023

TEKNOTAN

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Teh putih (Camellia sinensis L) merupakan teh yang dibuat dari pucuk daun teh muda yang masih menggulung tanpa fermentasi. Teh putih saat ini merupakan pilihan baru alternatif minuman di Indonesia dengan manfaat yang cukup banyak, memiliki banyak aktivitas diantaranya antioksidan, antimikroba dan antikolesterol. Teh putih juga memiliki aktivitas penghambatan terhadap enzim α-glucosidase dan xanthin oksidase. Senyawa utama teh putih yang diduga dalam aktivitas farmakologi tersebut adalah kelompok senyawa polifenol. Tujuan penelitian ini adalah analisis kadar fenol total dan flavonoid total dari beberapa teh yang beredar dipasaran. Metode penelitian meliputi makroskopis (organoleptik), skrining fitokimia dan penentuan fenol total, dan penentuan flavonoid total. Hasil penelitian dari sampel menunjukan bentuk teh putih menggulung terpilin, bau khas teh, dan rasa seduhan yang sepat. Teh putih juga memiliki kandungan kelompok senyawa metabolit sekunder fenol, flavonoid, tanin, saponin, alkaloid, dan triterpenoid/steroid. Teh putih sampel P-IRT 510320301146523 memiliki kandungan fenol total dan flavonoid total tertinggi yakni 5,826 ± 0,0370 mgGAE/g sampel dan 0,8061 ± 0,0183 mgQE/g sampel.

show abstract

Inhibition activity of Î±-glucosidase by infusion and ethanol extract of white tea and stevia combination

Cited by 3 publications

References 1 publication

Phytochemical Properties, Antioxidant Activity and α- Amilase Inhibitory of Curcuma Caesia

Phytochemical Properties, Antioxidant Activity and α- Amilase Inhibitory of Curcuma Caesia

Antidiabetic activity of herbal green tea extract from white mangrove (Avicennia marina) leaves towards blood glucose level of diabetic wistar rats (Rattus novergicus)

Analisis Fenol dan Flavonoid Total Pada Beberapa Teh Putih (Camellia sinensis L.) yang Beredar di Pasaran

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