Inhibition of chitin metabolism by avermectin in susceptible organisms.

Calcott, Peter H.; Fatig, Raymond

doi:10.7164/antibiotics.37.253

Cited by 41 publications

(8 citation statements)

References 10 publications

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“…Thus, it was surprising that we did not detect larger impacts on the more abundant members of the microbial community. Changes to the fungal community might have been expected, given that albendazole has antifungal activities, and avermectins have been shown to inhibit chitin synthesis 7 . However, anaerobic fungi occupy a specialised niche in the gut, and their physiology differs considerably to that of aerobic fungal pathogens.…”

Section: Discussionmentioning

confidence: 99%

Effects of long-acting, broad spectra anthelmintic treatments on the rumen microbial community compositions of grazing sheep

Moon

Carvalho

Kirk

et al. 2021

Sci Rep

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Anthelmintic treatment of adult ewes is widely practiced to remove parasite burdens in the expectation of increased ruminant productivity. However, the broad activity spectra of many anthelmintic compounds raises the possibility of impacts on the rumen microbiota. To investigate this, 300 grazing ewes were allocated to treatment groups that included a 100-day controlled release capsule (CRC) containing albendazole and abamectin, a long-acting moxidectin injection (LAI), and a non-treated control group (CON). Rumen bacterial, archaeal and protozoal communities at day 0 were analysed to identify 36 sheep per treatment with similar starting compositions. Microbiota profiles, including those for the rumen fungi, were then generated for the selected sheep at days 0, 35 and 77. The CRC treatment significantly impacted the archaeal community, and was associated with increased relative abundances of Methanobrevibacter ruminantium, Methanosphaera sp. ISO3-F5, and Methanomassiliicoccaceae Group 12 sp. ISO4-H5 compared to the control group. In contrast, the LAI treatment increased the relative abundances of members of the Veillonellaceae and resulted in minor changes to the bacterial and fungal communities by day 77. Overall, the anthelmintic treatments resulted in few, but highly significant, changes to the rumen microbiota composition.

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Section: Discussionmentioning

confidence: 99%

Effects of long-acting, broad spectra anthelmintic treatments on the rumen microbial community compositions of grazing sheep

Moon

Carvalho

Kirk

et al. 2021

Sci Rep

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“…This study shows firstly that the decrease of epidermal penetration of AVM is related to the upregulation of DmeCHS1/2 in AVM-resistant Drosophila larvae compared with that in the susceptible larvae. However, an earlier study showed that AVMs can inhibit chitin synthesis in Mucor miehei and Artemia salina [ 40 ]. The difference may be due to the different doses of AVM used in these studies.…”

Section: Discussionmentioning

confidence: 99%

Direct interaction of avermectin with epidermal growth factor receptor mediates the penetration resistance inDrosophilalarvae

et al. 2016

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With the widespread use of avermectins (AVMs) for managing parasitic and agricultural pests, the resistance of worms and insects to AVMs has emerged as a serious threat to human health and agriculture worldwide. The reduced penetration of AVMs is one of the main reasons for the development of the resistance to the chemicals. However, the detailed molecular mechanisms remain elusive. Here, we use the larvae of Drosophila melanogaster as the model organism to explore the molecular mechanisms underlying the development of penetration resistance to AVMs. We clearly show that the chitin layer is thickened and the efflux transporter P-glycoprotein (P-gp) is overexpressed in the AVM-resistant larvae epidermis. We reveal that the activation of the transcription factor Relish by the over-activated epidermal growth factor receptor (EGFR)/AKT/ERK pathway induces the overexpression of the chitin synthases DmeCHS1/2 and P-gp in the resistant larvae. Interestingly, we discover for the first time, to the best of our knowledge, that AVM directly interacts with EGFR and leads to the activation of the EGFR/AKT/ERK pathway, which activates the transcription factor Relish and induces the overexpression of DmeCHS1/2 and P-gp. These findings provide new insights into the molecular mechanisms underlying the development of penetration resistance to drugs.

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“…This effect can be induced at neuromuscular junctions (Wang & Pong, 1982;Campbell, 1985) and within the central nervous system (Lummis & Sattelle, 1985). Any effect upon chitin synthesis (Calcott & Fatig, 1984) has yet to be demonstrated in insects. Any effect upon chitin synthesis (Calcott & Fatig, 1984) has yet to be demonstrated in insects.…”

Section: Discussionmentioning

confidence: 99%

“…Recently it has been suggested that ivermectin acts directly upon chloride channels independently of GABA both in the central nervous system and at neuromuscular junctions (Tanaka & Matsumura, 1985). Any effect upon chitin synthesis (Calcott & Fatig, 1984) has yet to be demonstrated in insects.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of pupariation and adult development in Calliphora vomitoria treated with ivermectin

Strong¹

1986

Entomologia Exp Applicata

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Puparium formation and adult development in Calliphora vomitoria L. (Diptera: Calliphoridae) are seriously disrupted after treating final-stage larvae with ivermectin. Fifty 07o of larvae treated topically with 1.01/zg ivermectin 2 days before anticipated pupariation fail to form puparia, while the same response requires 2.24 #g given one day before pupariation. Permanent larvae so produced remain alive for up to 2weeks. Of the larvae that pupariate, there is disruption of adult development, leading to larval-adult intermediates. Only 0.1/~g ivermectin induces such disruption in 50~ of the treated larvae.The effects on puparium formation and adult development are independent. Inhibition of pupariation can be explained in terms of a disturbance of neuromuscular events leading up to pupariation. The effect on adult development is more difficult to explain. This work shows that ivermectin has great potential in clarifying the sequence of events leading up to puparation in cyclorrhaphous Diptera.

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Inhibition of chitin metabolism by avermectin in susceptible organisms.

Cited by 41 publications

References 10 publications

Effects of long-acting, broad spectra anthelmintic treatments on the rumen microbial community compositions of grazing sheep

Effects of long-acting, broad spectra anthelmintic treatments on the rumen microbial community compositions of grazing sheep

Direct interaction of avermectin with epidermal growth factor receptor mediates the penetration resistance inDrosophilalarvae

Inhibition of pupariation and adult development in Calliphora vomitoria treated with ivermectin

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