Inhibition of clinical and environmentalCryptococcus neoformans isolates by two Brazilian killer yeasts

Abstract

Two killer yeast strains (HB55 and HB88) capable of inhibiting human pathogenic fungi were isolated from leaves of Hibiscus rosa-sinensis in Brazil. These isolates were identified by conventional methods and sequencing of the D1/D2 region of the 26S rDNA as Kodamaea ohmeri. They inhibited all Cryptococcus neoformans (vars. neoformans, grubii and gattii) strains tested, including reference, clinical and environmental isolates. The killer phenotype was not cured by thermal treatment.

Typing of Staphylococcus epidermidis clinical strains by a selected panel of Brazilian killer yeasts

“…Sequences were assembled using phred/phrap/consed (http://www.phrap.org) and analyzed with Nucleotide-nucleotide BLAST (blastn) program [16], available in the site http://www.ncbi.nlm.nih.gov/ BLAST/. The killer yeast Kodamaea ohmeri KYHB55 was previously identified by sequencing of D1/D2 domains of the 26S rDNA gene [17]. Cultures were maintained at 4 °C in GYMP (Glucose 2%, Yeast Extract 0.5%, Malt Extract 2%, Monobasic Sodium Phosphate 0.2%, Agar 2%) slants covered with sterile mineral oil, and deposited at the yeast culture collection of the Microbiology Department of the Federal University of Rio Grande do Sul.…”

Ação antimicrobiana de micocinas produzidas por Wickerhamomyces anomalus: uma revisão / Antimicrobial action of mycocins produced by Wickerhamomyces anomalus: a review

“…Entretanto, esses valores podem ser diferentes dependendo das condições específicas de cada gênero, Brazilian Journal of Health Review, Curitiba, v.4, n.3, p. 10019-10029 may./jun. 2021 espécie ou linhagens de leveduras produtoras de micocinas (BUZZINI; TURCHETTI; VAUGHAN-MARTINI, 2007;FUENTEFRIA et al, 2006;MAGLIANI et al, 1997).…”