Inhibition of contact hypersensitivity with different analogs of benzoporphyrin derivative

Simkin, G; King, Diane E.; Levy, Julia G.; Chan, Agnes H.; Hunt, David W. C.

doi:10.1016/s0162-3109(97)00051-9

Cited by 26 publications

(26 citation statements)

References 30 publications

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“…Th2-like immune responses, characterized by the production of IL-4, IL-5, IL-6, IL-10, and IL-13 promote humoral responses, production of IgE and IgA, as well as activation of eosinophils, mast cells, and basophils (15,16). We suggested that subtoxic PDT with verteporfin might negatively modulate the CHS response (14,17), a Th1-like immune response, by stimulating the production of Th2-type cytokines. Splenocytes and draining lymph node cells from PDT-treated mice painted with DNFB released higher amounts of IL-10 than lymphoid cells from control DNFB-sensitized animals (17).…”

Section: P Hotodynamic Therapy (Pdt)mentioning

confidence: 93%

“…Pretreatment of skin grafts with subtoxic levels of verteporfin and light prolonged their acceptance on allogeneic recipients (13). Mice given verteporfin exhibited reduced CHS responses to DNFB under ambient light conditions as well as following whole body light irradiation, but not when protected from light (14). Immunologic reactivity to DNFB does develop in mice treated with subtoxic PDT, albeit at reduced levels.…”

Section: P Hotodynamic Therapy (Pdt)mentioning

confidence: 99%

“…Mice were sensitized and ear challenged to elicit CHS responses to DNFB as described (14,17). Briefly, CHS was induced on day 0 by applying 35 l of a DNFB (Sigma, St. Louis, MO) solution (0.5% DNFB in a 4:1 mixture of acetone and olive oil) with a micropipette to the inguinal region (14).…”

Section: Sensitization and Elicitation Of Chsmentioning

confidence: 99%

“…Briefly, CHS was induced on day 0 by applying 35 l of a DNFB (Sigma, St. Louis, MO) solution (0.5% DNFB in a 4:1 mixture of acetone and olive oil) with a micropipette to the inguinal region (14). The area was shaved before DNFB application.…”

Section: Sensitization and Elicitation Of Chsmentioning

confidence: 99%

“…and then placed in the dark for 1 h. For light treatments, mice were placed in clear plexiglass containers, and 15 J/cm 2 of red light (692 Ϯ 12 nm) at 12.5 mW/cm 2 was delivered from a pair of light emitting diodes (LED) panels (Hewlett Packard, San Jose, CA) positioned above and below the subject. PDT was given either 24 h before or 24 h after DNFB application, treatment times associated with strongly reduced CHS responses to DNFB (14).…”

Section: Pdt Cytokine and Ab Treatmentsmentioning

confidence: 99%

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IL-10 Contributes to the Inhibition of Contact Hypersensitivity in Mice Treated with Photodynamic Therapy

Simkin

Tao

Levy

et al. 2000

The Journal of Immunology

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We have explored the effect of photodynamic therapy (PDT) with verteporfin on the induction and expression of contact hypersensitivity (CHS) to 2,4-dinitrofluorobenzene (DNFB) in normal mice and IL-10-deficient mice. Our results indicate that DNFB sensitized mice given PDT with verteporfin and whole body red light irradiation exhibited a significant reduction in CHS compared with control animals. Administration of rIL-12 reversed the effect(s) of PDT as did treatment of mice with anti-IL-10-neutralizing Ab. Knockout mice deficient in IL-10 were found to be resistant to the inhibitory effects of PDT. In vitro proliferative responses using spleen cells from DNFB-sensitized and PDT-treated mice showed a significantly lower response to DNBS as compared with cells from DNFB-sensitized mice or DNFB and PDT-treated IL-10-deficient mice. Finally, naive mice exposed to PDT exhibited an increase in skin IL-10 levels, which peaked between 72 and 120 h post-PDT. Together these data support the role of IL-10 as a key modulator in the inhibition of the CHS response by whole body PDT.

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Section: P Hotodynamic Therapy (Pdt)mentioning

confidence: 93%

Section: P Hotodynamic Therapy (Pdt)mentioning

confidence: 99%

Section: Sensitization and Elicitation Of Chsmentioning

confidence: 99%

Section: Sensitization and Elicitation Of Chsmentioning

confidence: 99%

Section: Pdt Cytokine and Ab Treatmentsmentioning

confidence: 99%

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IL-10 Contributes to the Inhibition of Contact Hypersensitivity in Mice Treated with Photodynamic Therapy

Simkin

Tao

Levy

et al. 2000

The Journal of Immunology

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Amelioration of antigen-induced arthritis in rabbits by induction of apoptosis of inflammatory-cells with local application of transdermal photodynamic therapy

Ratkay

Chowdhary

Iamaroon

et al. 1998

Arthritis & Rheumatism

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Objective. To study the efficacy and mechanism of local transdermal photodynamic therapy (tPDT) in rabbits with antigen-induced arthritis (AIA).Methods. MA in rabbits on day 14 postinduction was treated with an intravenous injection of benzoporphyrin-derivative monoacid ring A (BPD; Verteporfin) and subsequent transdermal exposure of the knee joint to light. BPD uptake and PDT-induced apoptosis of the synovium was studied applying fluorescence confocal microscopy and immunohistochemistry. The (histo)pathology of the joints was assessed at day 28.Results. Treatment with tPDT resulted in significant amelioration of synovial inflammation and an almost complete prevention of pannus formation and bone and cartilage destruction. BPD uptake was detectable in activated T cells and macrophages, and there was significant PDT-induced increase in the number of apoptotic cells in the synovium.Conclusion. Because photodynamic therapy is both specific and noninvasive, our findings suggest that it could be used for treating arthritic joints in humans.

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The Immunosuppressive Activity of Pseudolaric Acid B on T lymphocytes in vitro

Wei

Tan

Hong

et al. 2012

Phytotherapy Research

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Pseudolaric acid B (PB) is a major bioactive component extracted from Pseudolarix kaempferi. Traditional medicine practitioners in China have been using the roots of this plant to treat inflammatory and microbial dermatoses for centuries. In this study, the experiments were conducted to investigate immunosuppressive activity of PB on T lymphocytes in vitro. The results show that PB has hypotoxicity on hepatocytes and renal cells assessed by Methyl thiazolyl tetrazoliym (MTT) Assay and selectively inhibits the proliferation of T lymphocytes and antigen-specific T lymphocytes stimulated by concanavalin A and dinitrophenyl-modified spleen cells, respectively. Then, PB also shows suppressive effect on T lymphocytes in mixed lymphocyte reaction. Moreover, PB can depress the production of interleukin-2 (IL-2) secreted by concanavalin A-activated T lymphocytes or dinitrophenyl-modified spleen cells-activated antigen-specific T lymphocytes. It is first found that PB exhibits the inhibitory action on antigen-specific T lymphocytes and on the production of IL-2 from antigen-specific T lymphocytes and selectively inhibits the proliferation of T lymphocytes and the production of IL-2 as well. PB is in need of further study because of its potent immunosuppressive function.

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Inhibition of contact hypersensitivity with different analogs of benzoporphyrin derivative

Cited by 26 publications

References 30 publications

IL-10 Contributes to the Inhibition of Contact Hypersensitivity in Mice Treated with Photodynamic Therapy

IL-10 Contributes to the Inhibition of Contact Hypersensitivity in Mice Treated with Photodynamic Therapy

Amelioration of antigen-induced arthritis in rabbits by induction of apoptosis of inflammatory-cells with local application of transdermal photodynamic therapy

The Immunosuppressive Activity of Pseudolaric Acid B on T lymphocytes in vitro

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