Inhibition of Curcumin-Treated Herpes Simplex Virus 1 and 2 in Vero Cells

Flores, Daniel J.; Lee, Lee H.; Adams, Sandra D.

doi:10.4236/aim.2016.64027

Cited by 25 publications

(24 citation statements)

References 39 publications

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“…Several natural compounds have been investigated as potential therapies. These natural products include eucalyptus extracts [34], polysaccharides and diglycerides [35], curcumin [36], and polyphenols from tea [24] Herpes simplex virus infections continue to affect a large percentage of the human population worldwide. There is no cure for HSV-1 infections and there remains a need to identify effective and affordable therapies to reduce the incidence.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol EGCG-Stearate

Patel¹,

Adams²,

Lee³

2018

ABB

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Epigallocatechin gallate (EGCG), a green tea polyphenol possesses antioxidant, antibacterial, anticancer and antiviral properties. EGCG-Stearate (EGCG-S) is of interest for this study because of its stability and lipophilic properties. The chemical modification of EGCG-S increased its lipid solubility. Herpes simplex virus-1 (HSV-1), a member of the family Herpesviridae, and Alphaherpesvirinae subfamily is a leading cause of human viral diseases in the United States. In this study, 25 μM, 50 μM, 75 μM, and 100 µM of EGCG and EGCG-S were used to carry out cytotoxicity, cell viability and cell proliferation assays to determine the maximum non-cytotoxic concentrations on cultured A549 cells. The results suggested that 75 µM of EGCG and EGCG-S is the appropriate concentration to further study the effect on the infection of HSV-1 in A549 cells. Infectivity, antiviral, and inverted microscopy assays were performed to study the effects of EGCG and EGCG-S on HSV-1 infection. An antiviral assay was performed using luminescence and it indicated that EGCG-S treated HSV-1 showed up to 90% inhibition. Confocal microscopy images further supported the inhibitory effects of 75 μM EGCG-S on HSV-1 infection in A549 cells. The long-term goal of this research is to use EGCG-S as a possible novel topical therapeutic treatment to limit the spread of HSV-1 infections.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol EGCG-Stearate

Patel¹,

Adams²,

Lee³

2018

ABB

Self Cite

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“…The cells tolerated up to 15 μg/mL and 60 μg/mL curcumin and Cur-CS nanoparticles, respectively. The cytotoxic effect of curcumin on different cells has been well demonstrated, where no cytotoxic effect was observed following exposure up to 30 μM curcumin [19,60,61]. The MTT results of the present study revealed that the Cur-CS nanoparticles had less cytotoxic effects than curcumin in a dose-and time-dependent Table 4, significant protective effects were observed in each stage; however, 20 μM curcumin and Cur-CS nanoparticles showed the highest protection percentage in cotreatment at 48 h, namely, 56% and 68%, respectively.…”

Section: Stability Studymentioning

confidence: 99%

In Vitro Evaluation of Curcumin‐Encapsulated Chitosan Nanoparticles against Feline Infectious Peritonitis Virus and Pharmacokinetics Study in Cats

Selvarajah

Hussein

et al. 2020

BioMed Research International

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Feline infectious peritonitis (FIP) is an important feline viral disease, causing an overridden inflammatory response that results in a high mortality rate, primarily in young cats. Curcumin is notable for its biological activities against various viral diseases; however, its poor bioavailability has hindered its potential in therapeutic application. In this study, curcumin was encapsulated in chitosan nanoparticles to improve its bioavailability. Curcumin-encapsulated chitosan (Cur-CS) nanoparticles were synthesised based on the ionic gelation technique and were spherical and cuboidal in shape, with an average particle size of 330 nm and +42 mV in zeta potential. The nanoparticles exerted lower toxicity in Crandell-Rees feline kidney (CrFK) cells and enhanced antiviral activities with a selective index (SI) value three times higher than that of curcumin. Feline-specific bead-based multiplex immunoassay and qPCR were used to examine their modulatory effects on proinflammatory cytokines, including tumour necrosis factor (TNF)α, interleukin- (IL-) 6, and IL-1β. There were significant decrements in IL-1β, IL-6, and TNFα expression in both curcumin and Cur-CS nanoparticles. Based on the multiplex immunoassay, curcumin and the Cur-CS nanoparticles could lower the immune-related proteins in FIP virus (FIPV) infection. The single- and multiple-dose pharmacokinetics profiles of curcumin and the Cur-CS nanoparticles were determined by high-performance liquid chromatography (HPLC). Oral delivery of the Cur-CS nanoparticles to cats showed enhanced bioavailability with a maximum plasma concentration (Cmax) value of 621.5 ng/mL. Incorporating chitosan nanoparticles to deliver curcumin improved the oral bioavailability and antiviral effects of curcumin against FIPV infection. This study provides evidence for the potential of Cur-CS nanoparticles as a supplementary treatment of FIP.

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“…Herpes simplex virus (HSV) is a highly contagious and endemic pathogen that causes herpes labialis, herpes genitalis, encephalitis, and keratitis [1]. HS viral infections are considered to be a significant health problem worldwide [2]. The HSV infection caused by both type 1 (HS-1) and type 2 (HS-2) viruses could be transmitted through close personal contact [3].…”

Section: Introductionmentioning

confidence: 99%

“…Turmeric (Curcuma longa Linn) is generally recognized as safe (GRAS) by the FDA [6] and is extensively used as a folk medicine for its antioxidant, anti-inflammatory, and antiseptic effects, particularly in Asian countries [7]. Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) is the main curcuminoid derived from turmeric rhizomes [2,8]. The therapeutic efficacy and safety of curcumin (up to 12 g/day) make it an attractive target for the prevention and treatment of different human diseases [9].…”

Section: Introductionmentioning

confidence: 99%

Development of Provesicular Nanodelivery System of Curcumin as a Safe and Effective Antiviral Agent: Statistical Optimization, In Vitro Characterization, and Antiviral Effectiveness

et al. 2020

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Curcumin is a natural compound that has many medical applications. However, its low solubility and poor stability could impede its clinical applications. The present study aimed to formulate dry proniosomes to overcome these pitfalls and improve the therapeutic efficacy of Curcumin. Curcumin-loaded proniosomes were fabricated by the slurry method according to 32 factorial design using Design-Expert software to demonstrate the impact of different independent variables on entrapment efficiency (EE%) and % drug released after 12 h (Q12h). The optimized formula (F5) was selected according to the desirability criteria. F5 exhibited good flowability and appeared, after reconstitution, as spherical nanovesicles with EE% of 89.94 ± 2.31% and Q12h of 70.89 ± 1.62%. F5 demonstrated higher stability and a significant enhancement of Q12h than the corresponding niosomes. The docking study investigated the ability of Curcumin to bind effectively with the active site of DNA polymerase of Herpes simplex virus (HSV). The antiviral activity and the safety of F5 were significantly higher than Curcumin. F5 improved the safety of Acyclovir (ACV) and reduced its effective dose that produced a 100% reduction of viral plaques. Proniosomes could be promising stable carriers of Curcumin to be used as a safe and efficient antiviral agent.

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Inhibition of Curcumin-Treated Herpes Simplex Virus 1 and 2 in Vero Cells

Cited by 25 publications

References 39 publications

Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol EGCG-Stearate

Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol EGCG-Stearate

In Vitro Evaluation of Curcumin‐Encapsulated Chitosan Nanoparticles against Feline Infectious Peritonitis Virus and Pharmacokinetics Study in Cats

Development of Provesicular Nanodelivery System of Curcumin as a Safe and Effective Antiviral Agent: Statistical Optimization, In Vitro Characterization, and Antiviral Effectiveness

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