Inhibition of Deoxynivalenol Translocation and Fungal Colonization in Fusarium Head Blight Resistant Wheat

Snijders, C. H. A.; Krechting, C. F.

doi:10.1007/978-94-011-2004-3_74

Cited by 31 publications

(31 citation statements)

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“…Snijders and Krechting (1992) found a correlation of r=0 .89 between visually assessed head blight and ergosterol levels in kernels for 22 bread wheat lines differing in resistance level, with a maximum ergosterol level of 112 .1 pg/g, and a correlation between ergosterol and DON of r=0 .85 . In our study the absence of any correlation between head blight rating and ergosterol or DON, and the high correlation between ergosterol and Fusarium-specific DON, suggest that ( earlier studies involving cereals .…”

Section: Discussionmentioning

confidence: 89%

“…Moreover, Van Eeuwijk et al (1995) showed that in bread wheat there is no specialisation of resistance to F culmorum, F graminearum or F nivale . Snijders & Krechting (1992) concluded that in bread wheat visual assessment of percentage infected spikelets gives a reliable estimate of the amount of fungal biomass for chaff at the time of observation, and for kernels at harvest time, except for the extremes of the I Second leaves inoculated with 2x10 6 spores/ml and scored 17 days after inoculation . 2 Days to (GS=59) from 1 January.…”

Section: Discussionmentioning

confidence: 99%

“…Tritordeum matured in general later, but was harvested at the time that the wheat heads were ready to harvest . Miller et al (1985) showed that ergosterol levels hardly increase the last two weeks before harvest and DON levels may even decline (Snijders & Krechting, 1992) . The effect of one harvest time for heads in different stages of ripening on ergosterol and DON-levels is, therefore, marginal .…”

Section: Discussionmentioning

confidence: 99%

“…As it was difficult to peel the glumes off the kernel of Hordeum chilense lines and some tritordeum lines, it was decided to grind the whole heads . Ground compound samples from the three blocks were sampled and analyzed for ergosterol, a measure of the fungal biomass (Seitz et al, 1979 ;Snijders & Krechting, 1992), and the Fusarium-produced toxin deoxynivalenol (DON) as described in Snijders & Krechting (1992) .…”

Section: Fusarium Culmorum Inoculation and Disease Assessmentmentioning

confidence: 99%

“…DON and 3-ADON are non-specific and increase the extent of disease symptoms and colonization but are not involved in the primary interaction that determines compatibility . The predominant head blight resistance mechanism involves prevention of fungal colonization while resistance to initial penetration is negligible (Snijders & Krechting, 1992 ; Snijders & Schepers, 1995) . Septoria glume blotch caused by Septoria nodorum (Berk .)…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Reaction of tritordeum to Fusarium culmorum and Septoria nodorum

Rubiales¹,

Snijders²,

Nicholson³

et al. 1996

Euphytica

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Fusarium Culmorum Inoculation and Disease Assessmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reaction of tritordeum to Fusarium culmorum and Septoria nodorum

Rubiales¹,

Snijders²,

Nicholson³

et al. 1996

Euphytica

Self Cite

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Assessing non-specificity of resistance in wheat to head blight caused by inoculation with European strains of Fusarium culmorum, F. graminearum and F. nivale using a multiplicative model for interaction

Eeuwijk

Mesterházy

Kling

et al. 1995

Theoret. Appl. Genetics

146

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To determine whether resistance to Fusarium head blight in winter wheat is horizontal and non-species specific, 25 genotypes from five European countries were tested at six locations across Europe in the years 1990, 1991, and 1992. The five genotypes from each country had to cover the range from resistant to susceptible. The locations involved were Wageningen, Vienna, Rennes, Hohenheim, Oberer Lindenhof, and Szeged. In total, 17 local strains of Fusarium culmorum, F. graminearum, and F. nivale were used for experimental inoculation. One strain, F. culmorum IPO 39-01, was used at all locations. Best linear unbiased predictions (BLUPs) for the head blight ratings of the genotypes were formed within each particular location for each combination of year and strain. The BLUPs over all locations were collected in a genotype-by environment table in which the genotypic dimension consisted of the 25 genotypes, while the environmental dimension was made up of 59 year-by-strain-by-location combinations. A multiplicative model was fitted to the genotype by-environment interaction in this table. The inverses of the variances of the genotype-by-environment BLUPs were used as weights. Interactions between genotypes and environments were written as sums of products between genotypic scores and environmental scores. After correction for year-by-location influence very little variation in environmental scores could be ascribed to differences between strains. This provided the basis for the conclusion that the resistance to Fusarium head blight in winter wheat was of the horizontal and non-species specific type. There was no indication for any geographical pattern in virulence genes. Any reasonable aggressive strain, a F. culmorum strain for the cool climates and a F. graminearum strain for the warmer humid areas, should be satisfactory for screening purposes.

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The use of ergosterol in the pathogenic fungusBipolaris sorokiniana for resistance rating of barley cultivars

et al. 1996

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Ergosterol content in the plant pathogenic fungus Bipolaris sorokiniana was determined in different matrices including mycelium, spores, culture filtrate and infected barley leaves. Ergosterol was extracted with methanol, hydrolysed with KOH and quantified by reverse phase high performance liquid chromatography (HPLC). Our procedure was used to study how the ergosterol concentration of B. sorokiniana varied due to fungal age and nutrient availability when growing in liquid medium. It was found that the ergosterol content decreased with fungal age. The decrease was not due to leakage. It was also found that a change to a less nutrient-rich medium caused an increase in ergosterol content whereas a change to a rich medium led to a decrease. The procedure was also used for quantification of fungal infections in complex matrices (e.g. leaves). The development of fungal infection in barley leaves was followed during 10 days. Visual grading of leaf spots was also compared to ergosterol content in three varieties of barley. The ergosterol content in the leaves increased exponentially until day 7, and the grading of the leaf spots was correlated to the ergosterol content. Our results show that, despite a great variation, ergosterol may be used as a biomarker to detect and quantify fungal infections in a given matrix.

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Inhibition of Deoxynivalenol Translocation and Fungal Colonization in Fusarium Head Blight Resistant Wheat

Cited by 31 publications

References 3 publications

Reaction of tritordeum to Fusarium culmorum and Septoria nodorum

Reaction of tritordeum to Fusarium culmorum and Septoria nodorum

Assessing non-specificity of resistance in wheat to head blight caused by inoculation with European strains of Fusarium culmorum, F. graminearum and F. nivale using a multiplicative model for interaction

The use of ergosterol in the pathogenic fungusBipolaris sorokiniana for resistance rating of barley cultivars

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