Inhibition of high glucose-induced protein mono-ADP-ribosylation restores neuritogenesis and sodium-pump activity in SY5Y neuroblastoma cells

Giulio, Anna Maria Di; Lesma, Elena; Germani, Elena; Gorio, Alfredo

doi:10.1002/(sici)1097-4547(19990901)57:5<663::aid-jnr8>3.0.co;2-6

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…For example, hyperglycemia’s up-regulation of ecto-ribosylation (Di Giulio et al , 1999) would facilitate NAD’s activation of P2X 7 purinoceptors. Also, it is known that diabetics can generate autoantibodies that block CD38 (Antonelli & Ferrannini, 2004), which is an ecto-NAD + -glycohydrolase that inhibits the ribosylation reactions (Krebs et al , 2005) required for the activation of P2X 7 purinoceptors by NAD + .…”

Section: Retinal Microvascular Physiology: New Insightsmentioning

confidence: 99%

Retinovascular physiology and pathophysiology: New experimental approach/new insights

Puro

2012

Progress in Retinal and Eye Research

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An important challenge in visual neuroscience is understand the physiology and pathophysiology of the intra-retinal vasculature, whose function is required for ophthalmoception by humans and most other mammals. In the quest to learn more about this highly specialized portion of the circulatory system, a newly developed method for isolating vast microvascular complexes from the rodent retina has opened the way for using techniques such as patch-clamping, fluorescence imaging and time-lapse photography to elucidate the functional organization of a capillary network and its pre-capillary arteriole. For example, the ability to obtain dual perforated-patch recordings from well-defined sites within an isolated microvascular complex permitted the first characterization of the electrotonic architecture of a capillary/arteriole unit. This analysis revealed that this operational unit is not simply a homogenous synctium, but has a complex functional organization that is dynamically modulated by extracellular signals such as angiotensin II. Another recent discovery is that a capillary and its pre-capillary arteriole have distinct physiological differences; capillaries have an abundance of ATP-sensitive potassium (KATP) channels and a dearth of voltage-dependent calcium channels (VDCCs) while the converse is true for arterioles. In addition, voltage transmission between abluminal cells and the endothelium is more efficient in the capillaries. Thus, the capillary network is well-equipped to generate and transmit voltages, and the pre-capillary arteriole is well-adapted to transduce a capillary-generated voltage into a change in abluminal cell calcium and thereby, a vasomotor response. Use of microvessels isolated from the diabetic retina has led to new insights concerning retinal vascular pathophysiology. For example, soon after the onset of diabetes, the efficacy of voltage transmission through the endothelium is diminished; arteriolar VDCCs is inhibited, and there is increased vulnerability to purinergic vasotoxicity, which is a newly identified pathobiological mechanism. Other recent studies reveal that KATP channels not only have an essential physiological role in generating vasomotor responses, but their activation substantially boosts the lethality of hypoxia. Thus, the pathophysiology of the retinal microvasculature is closely linked with its physiology.

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Section: Retinal Microvascular Physiology: New Insightsmentioning

confidence: 99%

Retinovascular physiology and pathophysiology: New experimental approach/new insights

Puro

2012

Progress in Retinal and Eye Research

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“…In view of its important housekeeping and specialized functions, it is expected that the Na,K-ATPase is involved in the neuritegenesis (Arakawa et al 1991;Di Giulio et al 1999). It is also conceivable that the Na,K-ATPase may have some physiological roles in the nerve regeneration process.…”

mentioning

confidence: 99%

“…In view of its important housekeeping and specialized functions, it is expected that the Na,K‐ATPase is involved in the neuritegenesis (Arakawa et al . 1991; Di Giulio et al . 1999).…”

mentioning

confidence: 99%

Na,K‐ATPase α3 subunit in the goldfish retina during optic nerve regeneration

Liu¹,

Matsukawa²,

Arai³

et al. 2002

Journal of Neurochemistry

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The goldfish optic nerve can regenerate after injury. To understand the molecular mechanism of optic nerve regrowth, we identified genes whose expression is specifically up-regulated during the early stage of optic nerve regeneration. A cDNA library constructed from goldfish retina 5 days after transection was screened by differential hybridization with cDNA probes derived from axotomized or normal retina. Of six cDNA clones isolated, one clone was identified as the Na,K-ATPase catalytic subunit a3 isoform by highsequence homology. In northern hybridization, the expression level of the mRNA was significantly increased at 2 days and peaked at 5-10 days, and then gradually decreased and returned to control level by 45 days after optic nerve transection. Both in situ hybridization and immunohistochemical staining have revealed the location of this transient retinal change after optic nerve transection. The increased expression was observed only in the ganglion cell layer and optic nerve fiber layer at 5-20 days after optic nerve transection. In an explant culture system, neurite outgrowth from the retina 7 days after optic nerve transection was spontaneously promoted. A low concentration of ouabain (50-100 nM) completely blocked the spontaneous neurite outgrowth from the lesioned retina. Together, these data indicate that up-regulation of the Na,K-ATPase a3 subunit is involved in the regrowth of ganglion cell axons after axotomy.

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“…CD157 was suggested to display a similar expression tendency in myeloid cells [48]. Since hyperglycemia directly enhances protein ADP-ribosylation in cultured neuroblastoma cells [49], resulting in increased ADPRCA in diabetic subjects, we speculate that decreased ADPRCA in PBMCs could reflect decreased suppressive effects of CD38 and CD157 and increased numbers of differentiated cells.…”

Section: Discussionmentioning

confidence: 91%

Decreased ADP‐Ribosyl Cyclase Activity in Peripheral Blood Mononuclear Cells from Diabetic Patients with Nephropathy

Ohtsuji

Yagi

Shintaku-Kubota

et al. 2008

Journal of Diabetes Research

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Aims/hypothesis. ADP-ribosyl-cyclase activity (ADPRCA) of CD38 and other ectoenzymes mainly generate cyclic adenosine 5’diphosphate-(ADP-) ribose (cADPR) as a second messenger in various mammalian cells, including pancreatic beta cells and peripheral blood mononuclear cells (PBMCs). Since PBMCs contribute to the pathogenesis of diabetic nephropathy, ADPRCA of PBMCs could serve as a clinical prognostic marker for diabetic nephropathy. This study aimed to investigate the connection between ADPRCA in PBMCs and diabetic complications. Methods. PBMCs from 60 diabetic patients (10 for type 1 and 50 for type 2) and 15 nondiabetic controls were fluorometrically measured for ADPRCA based on the conversion of nicotinamide guanine dinucleotide (NGD+) into cyclic GDP-ribose. Results. ADPRCA negatively correlated with the level of HbA1c (P = .040, R 2 = .073), although ADPRCA showed no significant correlation with gender, age, BMI, blood pressure, level of fasting plasma glucose and lipid levels, as well as type, duration, or medication of diabetes. Interestingly, patients with nephropathy, but not other complications, presented significantly lower ADPRCA than those without nephropathy (P = .0198) and diabetes (P = .0332). ANCOVA analysis adjusted for HbA1c showed no significant correlation between ADPRCA and nephropathy. However, logistic regression analyses revealed that determinants for nephropathy were systolic blood pressure and ADPRCA, not HbA1c. Conclusion/interpretation. Decreased ADPRCA significantly correlated with diabetic nephropathy. ADPRCA in PBMCs would be an important marker associated with diabetic nephropathy.

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Inhibition of high glucose-induced protein mono-ADP-ribosylation restores neuritogenesis and sodium-pump activity in SY5Y neuroblastoma cells

Cited by 6 publications

References 33 publications

Retinovascular physiology and pathophysiology: New experimental approach/new insights

Retinovascular physiology and pathophysiology: New experimental approach/new insights

Na,K‐ATPase α3 subunit in the goldfish retina during optic nerve regeneration

Decreased ADP‐Ribosyl Cyclase Activity in Peripheral Blood Mononuclear Cells from Diabetic Patients with Nephropathy

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