Inhibition of kinesin family member 20B sensitizes hepatocellular carcinoma cell to microtubule‐targeting agents by blocking cytokinesis

Liu, Xinran; Li, Yangkai; Zhang, Xia; Liu, Xinyuan; Peng, Anlin; Chen, Yu‐Chen; Meng, Lijing; Chen, Hong; Miao, Xiaoping; Zheng, Ling; Huang, Kun

doi:10.1111/cas.13794

Cited by 20 publications

(22 citation statements)

References 43 publications

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“…A recent study suggested to target the KIF20B gene in the treatment for hepatocellular carcinoma (38). Inhibition of KIF20B can block mitosis at both metaphase and telophase, which enhance the cytotoxicity of two chemotherapeutic drugs, hydroxycamptothecin, and mitomycin C (39). The role of KIF20B in tumorigenesis of meningiomas, especially multiple lesions, suggests that its suppression might be a novel strategy in the treatment for multiple meningiomas in the future.…”

Section: Discussionmentioning

confidence: 99%

High Copy-Number Variation Burdens in Cranial Meningiomas From Patients With Diverse Clinical Phenotypes Characterized by Hot Genomic Structure Changes

Hong

Chen

et al. 2020

Front. Oncol.

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Meningiomas, as the most common primary tumor of the central nervous system, are known to harbor genomic aberrations that associate with clinical phenotypes. Here we performed genome-wide genotyping for cranial meningiomas in 383 Chinese patients and identified 9,821 copy-number variations (CNVs). Particularly, patients with diverse clinical features had distinct tumor CNV profiles. CNV burdens were greater in high-grade (WHO grade II and III) samples, recurrent lesions, large tumors (diameter >4.3 cm), and those collected from male patients. Nevertheless, the level of CNV burden did not relate to tumor locations, peritumoral brain edema, bone invasion, or multiple lesions. Overall, the most common tumor CNVs were the copy-number gain (CNG) at 22q11.1 and the copy-number losses (CNLs) at 22q13.2, 14q11.2, 1p34.3, and 1p31.3. Recurrent lesions were featured by the CNLs at 1p31.3, 6q22.31, 9p21.3, and 11p12, and high-grade samples had more CNVs at 4q13.3 and 6q22.31. Meanwhile, large tumors were more likely to have the CNVs at 1p31.3 and 1p34.3. Additionally, recurrence prediction indicated the CNLs at 4p16.3 (p = 0.009, hazard ratio = 5.69) and 10p11.22 (p = 0.037, hazard ratio = 4.53) were candidate independent risk factors.

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Section: Discussionmentioning

confidence: 99%

High Copy-Number Variation Burdens in Cranial Meningiomas From Patients With Diverse Clinical Phenotypes Characterized by Hot Genomic Structure Changes

Hong

Chen

et al. 2020

Front. Oncol.

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“…Overexpression of KIFs might result in a series of errors including excessive spindle separation, premature separation of sister chromatids, advanced leads, and eventually bipolar or unipolar spindles, while lost expression of KIFs might lead to the opposite result (28). Abnormalities in these functions might eventually lead to the development of different diseases, including tumors (3–10). KIF20A was reported to promote the invasiveness of pancreatic cancer by regulating the transport of insulin like growth-binding protein 3-containing stress granules (29–32).…”

Section: Discussionmentioning

confidence: 99%

“…KIF23 could also promote glioma progression and being regulated by transcription factor 4 (37,38). As a member of kinesin-6 family, KIF20B functions as cancer-testis antigen specific to human bladder cancer (4,5), which is associated with cell proliferation and tumor growth in hepatocellular carcinoma through targeting p53, serving a crucial role in cancer (6–10). However, unlike KIF20A, there are few studies on the function and mechanism of KIF20B in tumors (4,6–10), especially in tongue cancer.…”

Section: Discussionmentioning

confidence: 99%

“…KIF20B was recently found to serve important roles in multiple types of cancer, which could function as cancer-testis antigen specific to human bladder cancer (4,6), and KIF20B was associated with cell proliferation, apoptosis and tumor growth in hepatocellular carcinoma targeting p53 (7). Several studies have indicated that KIF20B serves a role in tumor progression, including colorectal cancer, hepatocellular carcinoma, pancreatic cancer and bladder cancer (4,6–10). In addition, KIF20B predicts poor prognosis, and is associated with cell proliferation, apoptosis and tumor growth in hepatocellular carcinoma through targeting p53 (7,10).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have indicated that KIF20B serves a role in tumor progression, including colorectal cancer, hepatocellular carcinoma, pancreatic cancer and bladder cancer (4,6–10). In addition, KIF20B predicts poor prognosis, and is associated with cell proliferation, apoptosis and tumor growth in hepatocellular carcinoma through targeting p53 (7,10). However, the function and mechanism of KIF20B in different tumors has not been fully elucidated, and further discussions are still needed.…”

Section: Introductionmentioning

confidence: 99%

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Kinesin family member 20B regulates tongue cancer progression by promoting cell proliferation

Wang

2019

Mol Med Report

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Oral cancer refers to the malignant tumors that occur in the oral cavity, of which 80% are squamous cell carcinomas. The incidence of oral cancer accounts for ~5% of the incidence of systemic malignancies, with rapid progression, extensive infiltration and poor prognosis. In the present study, Kinesin family member (KIF)20B, a member of Kinesin-6 family, was identified as a potential biomarker which could promote cancer progression. A total of 82 patients were recruited and KIF20B expression levels were investigated by immunohistochemistry, and were divided into high and low groups based on the median of KIF20B expression levels. The clinicopathological features and survival-associated data of the two groups were analyzed and the results were provided as a table and by a Kaplan-Meier plot, respectively. Additionally, KIF20B was successfully silenced in two tongue cancer cell lines, CAL-27 and TCA-8113. MTT and colony formation assay were performed to determine the changes of cell proliferation in knocked down-KIF20B cell lines. In addition, proliferation-associated proteins Ki67 and PCNA were investigated, by western blotting. In animal experiments, subcutaneous tumor formation was performed with control cells and cells with knocked down KIF20B, to determine the inhibitory effect of KIF20B in vivo . Firstly, it was found that there was significantly high expression levels of KIF20B in tongue cancer patients (P<0.05). Patients with high expression of KIF20B had poorer clinicopathological results including tumor differentiation level, lymph node metastasis and clinical stages. The overall survival and relapse-free survival of high-expression group were also poor. Secondly, after successful establishment of cells with knocked down KIF20B, this resulted in a notable reduction in cell proliferation in vitro . Subsequent western blotting further confirmed that Ki67 and PCNA expression levels had a significant decline. Finally, it was demonstrated that knocking down KIF20B could inhibit tumor volume growth in vivo . In conclusion, the high level of KIF20B in oral squamous cell carcinoma was significantly associated with poor clinicopathological features and survival. KIF20B might promote cancer development through enhancing cell proliferation in vitro , and might be a potential biomarker of oral squamous cell carcinoma.

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KIF20B promotes the progression of clear cell renal cell carcinoma by stimulating cell proliferation

Xie

Zhu

et al. 2019

Journal Cellular Physiology

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Renal cell carcinoma (RCC) is a common urinary system cancer with high morbidity and mortality rate. Clear cell renal cell carcinoma (ccRCC) is a highly aggressive and common type of RCC. More and effective therapeutic targets are badly needed for the treatment of ccRCC. Kinesin family protein (KIF)20B, also named M‐phase phosphoprotein 1, was reported as a microtubule‐associated, plus‐end‐directed kinesin. KIF20B was involved in multiple cellular processes such as cytokinesis. Multiple studies indicated the oncogenic role for KIF20B in several types of tumors, including breast cancer and bladder cancer. However, the possible role of KIF20B in the progression of renal carcinoma is still unknown. Herein, our study demonstrated that KIF20B was relatively highly expressed in ccRCC tissues. In addition, KIF20B was inversely related to the clinical features including tumor size and T stage. We further found that inhibition of the KIF20B expression by a specific short hairpin RNA obviously reduces proliferation of ccRCC cells both in vitro and in vivo. Our study reveals the involvement of KIF20B in ccRCC progression. Generally, KIF20B is a promising novel therapeutic for the treatment of clear cell RCC.

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Inhibition of kinesin family member 20B sensitizes hepatocellular carcinoma cell to microtubule‐targeting agents by blocking cytokinesis

Cited by 20 publications

References 43 publications

High Copy-Number Variation Burdens in Cranial Meningiomas From Patients With Diverse Clinical Phenotypes Characterized by Hot Genomic Structure Changes

High Copy-Number Variation Burdens in Cranial Meningiomas From Patients With Diverse Clinical Phenotypes Characterized by Hot Genomic Structure Changes

Kinesin family member 20B regulates tongue cancer progression by promoting cell proliferation

KIF20B promotes the progression of clear cell renal cell carcinoma by stimulating cell proliferation

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