Inhibition of microbial biofilm formation by Cydonia oblonga Mill. fruit peel and leaf ethanolic extracts

Abstract

Biological activities of Sorbus aucuparia L. leaves extract were tested in our study. This study aimed to investigate the activity of Cydonia oblonga Mill. peels and leaf ethanolic extracts on biofilm formation of different microbial strains, including pathogenic bacteria and food poisoning strains. It was shown that both of the investigated extracts inhibited biofilm formation in a dose dependent manner with sub-minimal inhibitory concentrations. The percentage of biofilm formation inhibition varied for each bacterial strain and was in the range of 10-100%, for both of the tested extracts. The ability of extracts to inhibit already formed biofilms presented as minimal concentration necessary to disrupt biofilms and concentrations was in the range of 10-100 µg/mL for the leaf extract and 5-75 µg/mL for the peel extract. The investigated extracts showed a promising antimicrobial effect comparable to, or even higher than the used reference compounds, which make these plant parts attractive for pharmaceutical and food industry.

Valorization of quince peel into functional food ingredients: A path towards “zero waste” and sustainable food systems

“…Stojkovic et al. [ 54 ] investigate the effects of quince peel and leaf ethanolic extracts on biofilm formation and verified its ability to disrupt already formed biofilms (including those of Escherichia coli , Listeria monocytogenes , Staphylococcus aureus , and Salmonella Typhimurium), with a MIC in the range of 10–100 μg/mL for leaf extract and 5–60 μg/mL for peel extract. These results were comparable to or even higher than the reference controls.…”

Upcycling Quince Peel into Bioactive Ingredients and Fiber Concentrates through Multicomponent Extraction Processes

“…Other studies have attributed greater antimicrobial activity to aqueous acetone extracts from quince peel than from its pulp, especially against S. aureus and P. aeruginosa, but also E. coli and Candida albicans [11]. Quince peel ethanolic extracts have also been described as having the ability to disrupt biofilms formed by E. coli, L. monocytogenes, S. aureus, and S. Typhimurium, with MIC ranging from 5 to 60 µg/mL [55]. According to other studies, although the entry of malic acid into the bacterial cell is limited by its low lipid solubility, it may enter the cells when undissociated and change the internal pH of the microorganism, causing antimicrobial effects [56].…”

Antibacterial and antibiofilm activity of selected polyphenolic compounds: An in vitro study on Staphylococcus aureus

“…The main constituents of biofilm matrix include oligosaccharides, DNA, proteins and teichoic acids while a cyclic peptide, autoinducing peptide, serves as a messenger in the intercellular bacterial communication during biofilm establishment (Craft et al, 2019). Recent studies have highlighted the potential of different agents against S. aureus biofilms including mushrooms (Kostić et al, 2020b), fruit peel (Stojković et al, 2018), acidic amino acids (Warraich et al, 2020) and essential oils (Kerekes et al, 2019), but the search for efficient antibiofilm therapeutics is still running.…”