Inhibition of neutrophil recruitment by ES of <i>Nippostrongylus brasiliensis</i>

Keir, Pamela A; Brown, David M.; Clouter-Baker, Anna; Harcus, Yvonne; Proudfoot, Lorna

doi:10.1111/j.0141-9838.2004.00692.x

Cited by 21 publications

(23 citation statements)

References 12 publications

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“…The literature reveals that both inhibitory and chemoattractant parasite-derived molecules have been described. However, a closer examination of the published data unveiled that tissue-dwelling parasites (usually adults) often release inhibitory factors, possibly in an attempt to reduce the local immune response (Alkarmi and Behbehani, 1989;Culley et al, 2000;Keir et al, 2004;Leid et al, 1987;Lo et al, 1999). Conversely, tissue-migrating larvae usually secrete compounds that promote inflammation (Falcone et al, 2001;Horii et al, 1988;Niwa et al, 1998;Owhashi et al, 1985;Owhashi et al, 1997;Rubio de Kromer et al, 1998;Tanaka et al, 1979;Tanaka and Torisu, 1978).…”

Section: Discussionmentioning

confidence: 99%

Necator americanus: The Na-ASP-2 protein secreted by the infective larvae induces neutrophil recruitment in vivo and in vitro

Bower¹,

Constant²,

Méndez³

2008

Experimental Parasitology

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The L3-secreted Ancylostoma Secreted Protein-2 from the human hookworm Necator americanus (Na-ASP-2) has been selected as a candidate vaccine antigen in anticipation of clinical trials. Its crystal structure revealed that Na-ASP-2 has structural and charge similarities to CC-chemokines, suggesting that it might act as a chemokine mimic when released by the infective larvae during tissue migration. Using the air pouch model of acute inflammation, we found that Na-ASP-2 induced a significant leukocyte influx to the skin pouch, mostly comprised of neutrophils (60%) and monocytes (30%) that was transient and resolved in 24 h. Other hookworm larval proteins did not cause any inflammatory leukocytes to migrate into air pouches. In vitro chemotaxis assays confirmed our results and demonstrated that leukocyte migration was a direct effect of Na-ASP-2 exposure and not caused by other molecules released by host cells in the inflammatory microenvironment or by the expression vector.

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Section: Discussionmentioning

confidence: 99%

Necator americanus: The Na-ASP-2 protein secreted by the infective larvae induces neutrophil recruitment in vivo and in vitro

Bower¹,

Constant²,

Méndez³

2008

Experimental Parasitology

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“…Both N. brasiliensis infection [60] and NES alone can inhibit allergen-induced lung inflammation [61]. In vivo studies showed that ES from N. brasiliensis L3 larvae (L-NES) inhibited LPS-dependent neutrophil recruitment to the lungs [62]. Despite the protective effects of NES against lung inflammation, L-NES is intrinsically allergenic [63], suggesting that different components may be acting in opposing manners over the longer term.…”

Section: Functional and Molecular Analyses Of Helminth Productsmentioning

confidence: 99%

Helminth immunoregulation: The role of parasite secreted proteins in modulating host immunity

Hewitson

Grainger

Maizels

2009

Molecular and Biochemical Parasitology

644

590

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Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive effector responses are muted, allowing survival of the parasite in a “modified Th2” environment. Drug treatment to clear the worms reverses the immunoregulatory effects, indicating that a state of active suppression is maintained by the parasite. Hence, research has focussed on “excretory–secretory” products released by live parasites, which can interfere with every aspect of host immunity from initial recognition to end-stage effector mechanisms. In this review, we survey our knowledge of helminth secreted molecules, and summarise current understanding of the growing number of individual helminth mediators that have been shown to target key receptors or pathways in the mammalian immune system.

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“…also identified a neutrophil inhibitory factor (NIF) from Ancyslostoma caninum, which binds CD11b/CD18 and there is a homologue in A. ceylanicum (161,162). Fasciola hepatica is known to secrete a factor that inhibits superoxide output from activated neutrophils (163) and secretory products from L3 of N. brasiliensis (164) also inhibit neutrophil recruitment into the bronchoalveolar lavage.…”

Section: Neutrophilsmentioning

confidence: 99%

Granulocytes: effector cells or immunomodulators in the immune response to helminth infection?

Cadman

Lawrence

2010

Parasite Immunology

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Granulocytes are effector cells in defence against helminth infections. We review the current evidence for the role of granulocytes in protective immunity against different helminth infections and note that for each parasite species the role of granulocytes as effector cells can vary. Emerging evidence also points to granulocytes as immunomodulatory cells able to produce many cytokines, chemokines and modulatory factors which can bias the immune response in a particular direction. Thus, the role of granulocytes in an immunomodulatory context is discussed including the most recent data that points to an important role for basophils under this guise.

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Inhibition of neutrophil recruitment by ES of Nippostrongylus brasiliensis

Cited by 21 publications

References 12 publications

Necator americanus: The Na-ASP-2 protein secreted by the infective larvae induces neutrophil recruitment in vivo and in vitro

Necator americanus: The Na-ASP-2 protein secreted by the infective larvae induces neutrophil recruitment in vivo and in vitro

Helminth immunoregulation: The role of parasite secreted proteins in modulating host immunity

Granulocytes: effector cells or immunomodulators in the immune response to helminth infection?

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