Inhibition of nitric oxide (NO•) production in murine macrophages by flavones

Król, Wojciech; Czuba, Zenon; Threadgill, Michael D.; Cunningham, Bernadette; Pietsz, Grazyna

doi:10.1016/0006-2952(95)00237-t

Cited by 82 publications

(49 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flavone and several other amino-substituted flavones were reported to inhibit NO production (44). Genistein was proved to inhibit LPS-induced NO production in macrophages (45).…”

Section: Effects On the Expression Of Inos And Cox-2mentioning

confidence: 99%

Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms

et al. 2004

View full text Add to dashboard Cite

Abstract. Plant flavonoids show anti-inflammatory activity in vitro and in vivo. Although not fully understood, several action mechanisms are proposed to explain in vivo anti-inflammatory action. One of the important mechanisms is an inhibition of eicosanoid generating enzymes including phospholipase A 2 , cyclooxygenases, and lipoxygenases, thereby reducing the concentrations of prostanoids and leukotrienes. Recent studies have also shown that certain flavonoids, especially flavone derivatives, express their anti-inflammatory activity at least in part by modulation of proinflammatory gene expression such as cyclooxygenase-2, inducible nitric oxide synthase, and several pivotal cytokines. Due to these unique action mechanisms and significant in vivo activity, flavonoids are considered to be reasonable candidates for new anti-inflammatory drugs. To clearly establish the therapeutic value in inflammatory disorders, in vivo anti-inflammatory activity, and action mechanism of varieties of flavonoids need to be further elucidated. This review summarizes the effect of flavonoids on eicosanoid and nitric oxide generating enzymes and the effect on expression of proinflammatory genes. In vivo anti-inflammatory activity is also discussed. As natural modulators of proinflammatory gene expression, certain flavonoids have a potential for new anti-inflammatory agents.

show abstract

“…Flavone and several other amino-substituted flavones were reported to inhibit NO production (44). Genistein was proved to inhibit LPS-induced NO production in macrophages (45).…”

Section: Effects On the Expression Of Inos And Cox-2mentioning

confidence: 99%

Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms

et al. 2004

View full text Add to dashboard Cite

show abstract

“…In this regard, it is very interesting that several studies have shown that specific flavonoids can suppress the induction of iNOS gene and protein expression, and NO • production by cytokines and endotoxins in mouse macrophage RAW 264.7 cells [99,109,152]. The mechanism did not include a direct inhibitory effect on enzyme activity, but rather the modulation of cell signaling pathways necessary for NOS gene expression.…”

Section: Nitric Oxide and Mapk Signalingmentioning

confidence: 99%

“…Recent reports demonstrated that the induction of iNOS expression following treatment with LPS or interferon-␥ [108], as well as the expression of nNOS requires the ERK1/2 signaling pathway, providing a potentially interesting link between MAPK signaling, nitric oxide production, and mitochondrial function. Since epicatechin and kaempferol have been shown to attenuate the oxidative stress-mediated activation of ERK1/2 [178] it remains to be established whether or not these flavonoids are capable of suppressing the production of NO • in neurons and glial cells in vivo as reported for iNOS in cultured macrophages [99,109,154], and what influences this may have on cellular outcomes of oxidative insults.…”

Section: Flavonoids: Neuroprotective Agents In Vivo and In Vitro?mentioning

confidence: 99%

MAPK signaling in neurodegeneration: influences of flavonoids and of nitric oxide

Schroeter

Boyd

Spencer

et al. 2002

Neurobiology of Aging

310

178

View full text Add to dashboard Cite

Oxidative and nitrosative stress is increasingly associated with the pathology of neurodegeneration and aging. The molecular mechanisms underlying oxidative/nitrosative stress-induced neuronal damage are emerging and appear to involve a mode of death in which mitogen-activated protein kinase (MAPK) signaling pathways are strongly implicated. Thus, attention is turning towards the modulation of intracellular signaling as a therapeutic approach against neurodegeneration. Both endogenous and dietary agents have been suggested as potent modulators of intracellular signal transduction, e.g. nitric oxide and flavonoids, respectively. This review addresses recent findings on the biological effects of flavonoids and nitric oxide in neurodegeneration and aims to elucidate the rationale for their prospective use as modulators of cellular signal transduction. © 2002 Elsevier Science Inc. All rights reserved.Keywords: Apoptosis; Oxidative stress; Neuron; Flavonoids; MAP kinase; JNK; ERK; Epicatechin; Nitric oxide; Mitochondria; Redox status; Polyphenols Abbreviations: AKT, serine/threonine kinase (also known as protein kinase B); AP-1, activator protein-1; Apaf-1, apoptosis protease activating factor-1; ASK1, apoptosis signal-regulating kinase-1; Bad, Bcl-2/BclX Lassociated death promoting protein; Bax, pro-apoptotic protein of the Bcl-2 family; Bcl-2, B-cell lymphoma 2: protein with anti-apoptotic properties; BclX L , long form of Bclx: anti-apoptotic protein of the Bcl-2 family; Caspase, cysteinyl aspartic acid-protease; c-Jun, mammalian equivalent of Jun: part of the AP-1 transcription complex; CREB, cAMP response element binding protein; DIABLO/smac, mitochondria-related pro-apoptotic protein: inhibits XIAP; ERK1/2, extracellular signal-related kinase; Fas, CD95: member of the TNF receptor family; GSHST, glutathione Stransferase; JNK, c-Jun amino-terminal kinase; MAPK, mitogen-activated protein kinase; MAPKK, MAPK kinase; MAPKKK, MAPKK kinase; MEK1/2, ERK1/2-specific MAPKK; MKK4/7, JNK-specific MAPKK; MSK1, mitogen-and stress-activated kinase-1; NF-B, nuclear factor of immunoglobulin k locus in B-cells; ONOO − , peroxynitrite anion; p53, pro-apoptotic tumor suppressor gene product; PI3-kinase, phosphoinositol 3-kinase; Ras, small G-protein; RNS, reactive nitrogen species; ROS, reactive oxygen species; RSK, pp90 ribosomal S6 kinase; SAPK, stressactivated protein kinase; XIAP, X-linked inhibitor of apoptosis: inhibits the activation of caspase-9

show abstract

“…It has been identified as potent molecule that may exert regulatory or cytotoxic effects depending on the concentration acting on the target cell [16,17]. The results of our earlier studies [18] suggest that the flavones can modulate the immune responses and the inflammatory reactions by controlling production of nitric oxide. J-774A.1 cells are functional similar to murine macrophages [19,20].…”

Section: Introductionmentioning

confidence: 93%

The Influence of some Xanthone Derivatives on the Activity of J-774A.1 Cells

Marona¹,

Pękala²,

Gunia³

et al. 2009

Sci. Pharm.

View full text Add to dashboard Cite

The chemiluminescence of stimulated cells with phorbol myristate acetate and the production of nitric oxide after stimulation with lipopolisaccharide in the presence of the parent compounds FAA (flavone-8-acetic acid = (4-oxo-2-phenyl-4H-chromen-8-yl)acetic acid), XAA (xanthone-4-acetic acid = (9-oxo-9H-xanthen-4-yl)acetic acid), and appropriate xanthone derivatives (1-7) was determined. Also the toxicity of the FAA, MFAA ((6-methyl-4-oxo-2-aryl-4H-chromen-8-yl)acetic acid), XAA and 1-7 against J-774A.1 cultured cells was evaluated. Compound 5 (2-methyl-2-{[(9-oxo-9H-xanthen-2-yl)methyl]sulfanyl}-propanoic acid) was effective in inhibiting chemiluminescence of J-774A.1 cells but most of the other tested compounds stimulated the reaction. FAA and two xanthones with a methoxycarbonyl moeity slightly decreased the generation of nitric oxide at 50 μM. Most of the tested compounds (1-7) showed weak toxicity at concentration of 100 μM.

show abstract

Inhibition of nitric oxide (NO•) production in murine macrophages by flavones

Cited by 82 publications

References 24 publications

Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms

Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms

MAPK signaling in neurodegeneration: influences of flavonoids and of nitric oxide

The Influence of some Xanthone Derivatives on the Activity of J-774A.1 Cells

Contact Info

Product

Resources

About