Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples

Sabaté, Daniela C.; Carrillo, L.; Audisio, M

doi:10.1016/j.resmic.2009.03.002

Cited by 105 publications

(78 citation statements)

References 47 publications

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“…However, thanks to the ability of several Bacillus strains to produce antibiotics, bacteriocins, or antifungal compounds, they find application to pursue agricultural and healthcare purposes (Alfonzo et al, 2012;Duc et al, 2004). Some strains of P. polymyxa (Lee et al, 2009), B. subtilis (Sabatè et al, 2009), B. cereus, B. licheniformis and B. megaterium (Alippi and Reynaldi, 2006), isolated from honey samples or different apiarian sources, were successfully tested against Paenibacillus larvae. B. amyloliquefaciens is considered able to inhibit Ascosphaera apis (Brittes Benitez et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…B. amyloliquefaciens is considered able to inhibit Ascosphaera apis (Brittes Benitez et al, 2012). P. larvae and A. apis are two common pathogens causing American foulbrood and chalkbrood in honey bee colonies (Sabatè et al, 2009). Today diseases and parasites are considered one of the main reasons of the decrease in bee population (Genersch, 2010) and the selection of microbial strains with antagonistic effect represents an important strategy for the biological control of these dangerous organisms (Yoshiyama et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Cultivable microorganisms associated with honeys of different geographical and botanical origin

et al. 2014

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a b s t r a c tIn this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-ITS rRNA region for filamentous fungi. The results showed the presence of 13 species of bacteria, 5 of yeasts and 17 of filamentous fungi; the species most frequently isolated were Bacillus amyloliquefaciens, Zygosaccharomyces mellis and Aspergillus niger for the three microbial groups, respectively. The highest microbial diversity was found in multifloral honeys. No correlation among the microbial species and the botanical/geographical origin was found, but some strains were highly adapted to these matrices since they were found in several samples of different origin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cultivable microorganisms associated with honeys of different geographical and botanical origin

et al. 2014

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“…There is strong evidence of an increase of antimicrobial peptide (AMPs) production induced by honey bee gut bacteria (Evans & Lopez, 2004;Jefferson et al, 2013;Yoshiyama et al 2013;Janashia & Alaux, 2016). Several in vitro trials confirmed the hability of lactobacilli and bifidobacteria to inhibit honey bee pathogens, in particular Paenibacillus larvae, Melissococcus plutonius and Ascosphaera apis (Forsgren et al, 2009;Sabaté et al, 2009;Yoshiyama & Kimura, 2009;Vásquez et al, 2012). Similarly, a reduction in N. ceranae intensity was reported when a Bacillus spp.…”

Section: Introductionmentioning

confidence: 91%

Effects of Lactobacillus Johnsonii AJ5 Metabolites on Nutrition, Nosema Ceranae Development and Performance of Apis Mellifera L.

Piano

Maggi

Pellegrini

et al. 2017

Journal of Apicultural Science

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A b s t r a c t The European honey bee (Apis mellifera L.) is known to be affected by such stress factors as pathogen load, poor nutrition and depressed immunity. Nosema ceranae is one of the main parasites that affect colony populations. The relationship between the stress factors and honey bee-bacteria symbiosis appears as an alternative to enhance bee health. The aim of this study was to evaluate the effect of the oral administration of bacterial metabolites produced by Lactobacillus johnsonii AJ5 on nutritional parameters, the N. ceranae development and the performance of A. mellifera colonies. Laboratory assays were performed and demonstrated that the bacterial metabolites did not have a toxic effect on bees. Field trial showed an increase of colonies population over time. Also, a decreasing trend of fat bodies per bee was detected in all colonies but there were no evident changes on abdomen protein content at the end of the assay. Lastly, N. ceranae prevalence showed a tendency to reduce with the organic acids. Future studies should be performed to increase our knowledge of the physiological effects of bacterial metabolites on the health of bee colonies.

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“…In another study a mixture of surfactin and iturin disintegrated the cell wall of the gramnegative phytopathogen Xanthomonas campestris (Etchegaray et al, 2008). Surfactin was also shown to display antimicrobial activity against Paenibacillus larvae, an extremely contagious and dangerous pathogen of honeybees (Sabate et al, 2009). Surfactin is known to inhibit phospholipase A2, involved in the pathophysiology of inflammatory bowel disease, which is related to ulcerative colitis and Crohn's disease.…”

Section: Antibacterial Anti-inflammatory and Antifungal Effectsmentioning

confidence: 99%

Surfactin - Novel Solutions for Global Issues

Seydlová¹,

Čabala²,

Svobodová³

2011

Biomedical Engineering, Trends, Research and Technologies

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Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples

Cited by 105 publications

References 47 publications

Cultivable microorganisms associated with honeys of different geographical and botanical origin

Cultivable microorganisms associated with honeys of different geographical and botanical origin

Effects of Lactobacillus Johnsonii AJ5 Metabolites on Nutrition, Nosema Ceranae Development and Performance of Apis Mellifera L.

Surfactin - Novel Solutions for Global Issues

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