Inhibition of pro-inflammatory enzymes by medicinal plants from the Argentinean highlands (Puna)

Torres-Carro, Romina; Isla, Marı́a Inés; Thomas‐Valdés, Samanta; Jiménez‐Aspee, Felipe; Schmeda‐Hirschmann, Guillermo; Arena, Mario Eduardo

doi:10.1016/j.jep.2017.04.013

Cited by 34 publications

(28 citation statements)

References 32 publications

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“…The ethanol bark extract of F. indica showed the highest A5-LOX inhibitory activity followed by that of S. cochinchinesis over the other extracts. When compared with the reported A5-LOX inhibitory activity of Caffeic acid, (IC 50 : 57.0 μg/mL), [ 29 ] which is a known lipoxygenase inhibitor, the IC 50 values of bark extracts of F. indica and S. cochinchinesis are about 2.5 fold and 1.5 fold lower than that of caffeic acid, respectively so that could be considered as even more potent than caffeic acid as A5-LOX inhibitors. To best of our knowledge, no previous studies have been conducted on A5-LOX inhibitory potential of extracts of F. indica and S. cochinchinesis.…”

Section: Discussionmentioning

confidence: 99%

In vitro pro-inflammatory enzyme inhibition and anti-oxidant potential of selected Sri Lankan medicinal plants

Perera

Samarasekera

Handunnetti

et al. 2018

BMC Complement Altern Med

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BackgroundThe extracts of the ten selected Sri Lankan medicinal plants have been traditionally used in the treatment of inflammatory mediated diseases. The extracts were investigated for anti-inflammatory and anti-oxidant potential in vitro to identify bio-active extracts for further chemical characterization.MethodsIn vitro anti-inflammatory activities of total ethanol extracts were investigated measuring the inhibitory activities of four pro-inflammatory enzymes, arachidonate-5- lipoxygenase (A5-LOX), hyaluronidase (HYL), xanthine oxidase (XO) and inducible nitric oxide (iNO) synthase. Cytotoxicity of extracts were determined by MTT assay. Oxidative burst inhibition (OBI) on human whole blood (WB) and isolated polymorphoneutrophils (PMNs) was carried out for a selected bio-active extract. Anti- oxidant activities of the extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferric reducing antioxidant power (FRAP), ferrous ion chelation (FIC) and oxygen radical absorbance capacity (ORAC) assays. Total polyphenol and total Flavonoid contents of the extracts were also determined. The most active plant extract was analysed using Gas chromatography-Mass spectrometry (GC-MS) and High Performance Liquid Chromatography (HPLC).ResultsThe ethanol bark extract of Flacourtia indica showed the highest A5-LOX (IC50: 22.75 ± 1.94 g/mL), XO (70.46 ± 0.18%; 250 μg/mL) and iNOs inhibitory activities on LPS- activated raw 264.7 macrophage cells (38.07 ± 0.93%; 500 μg/mL) with promising OBI both on WB (IC50: 47.64 2.32 μg/mL) and PMNs (IC50: 5.02 0.38 μg/mL). The highest HYL inhibitory activity was showed by the leaf extracts of Barathranthus nodiflorus (42.31 ± 2.00%; 500 μg/mL) and Diospyros ebenum (41.60 ± 1.18%; 500 μg/mL). The bark and leaf extracts of Callophyllum innophyllum (IC50: 6.99 ± 0.02 μg/mL) and Symplocus cochinchinesis (IC50: 9.85 ± 0.28 μg/mL) showed promising DPPH free radical scavenging activities. The GC-MS analysis of ethanol bark extract of F. indica showed the presence of two major bio-active compounds linoleic acid ethyl ester and hexadecanoic acid, ethyl ester (> 2% peak area). The HPLC analysis showed the presence polyphenolic compounds.ConclusionThe ethanol bark extract of F. indica can be identified as a potential candidate for the development of anti-inflammatory agents, which deserves further investigations. The bio-active plant extracts may be effectively used in the applications of cosmetic and health care industry.

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Section: Discussionmentioning

confidence: 99%

In vitro pro-inflammatory enzyme inhibition and anti-oxidant potential of selected Sri Lankan medicinal plants

Perera

Samarasekera

Handunnetti

et al. 2018

BMC Complement Altern Med

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“…The chelation of ferrous ions by the extracts was determined according to Torres Carro et al [ 35 ]. Briefly, 6 μ L of 2 mM FeSO 4 was added to different concentration of the extracts (100–700 μ g/mL) or positive control Na 2 EDTA (5–20 μ g/mL) and ultrapure water to a final volume of 143 μ L. The reaction was initiated by the addition of 7 μ L of 5 mM ferrozine solution which forms a colored complex with Fe 2+ .…”

Section: Methodsmentioning

confidence: 99%

Effect of Wine Wastes Extracts on the Viability and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus Strains

Viola

Torres-Carro

Cartagena

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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In this work, we intended to inhibit the biofilm synthesis and the metabolism of Gram-positive and Gram-negative bacteria using two highly available wastes (stem and marc) obtained after the manufacturing of Torrontes wine at Cafayate, Argentina. Wine wastes contain a significant amount of bioactive compounds, mainly phenolic compounds, which makes them a potential source of compounds with beneficial properties to human health, as they could inhibit the virulence of pathogenic bacteria or protect the tissue against oxidative stress. Marc and stem extracts of Torrontes wine were evaluated for their ability to inhibit the metabolism and biofilm production of Pseudomonas aeruginosa and Staphylococcus aureus strains. The phytochemical composition and antioxidant activity of these extracts were also determined. The methanol and ethyl acetate extracts, which contained the highest amount of total polyphenolic, exhibited the highest scavenging capacity of ABTS and nitric oxide and the strongest Fe3+ reducing power and exhibited the highest level of inhibition of the biofilm formation and of the metabolic activity in bacterial biofilm. We also noticed a positive correlation between phenolic compounds content, the antioxidant activity, and the anti-biofilm capacity of the winemaking wastes. These results display the potentiality of wine wastes to prevent or reduce the formation of biofilm. Moreover, their abundance makes them an attractive and affordable source of antibiofilm and antioxidant agents.

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“…In this habitat grow many vegetal products that have developed a great variety of bioactive compounds as an adaptation to environmental stress, including the biosynthesis of secondary metabolites with relevant pharmacological activities (TIMMERMANN & HOFFMANN, 1985;KLEIER & LAMBRINOS, 2005). Vegetation is dominated by an alternation of grasses and shrubs that are used by the Andeans inhabitants as a source of food, medicine, forage, and building (TORRES-CARRO et al, 2017). One of the most abundant shrubs is the Chilka, a plant known by this name for the communities of the Andes.…”

Section: Introductionmentioning

confidence: 99%

Potential of Baccharis alnifolia Meyen & Walpan (Chilka) from northern Chile used as a medicinal infusion

et al. 2019

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Baccharis alnifolia Meyen & Walp. is a native and medicinal, Chilean Altiplano herb that is used as a poultice and also consumed as an infusion to relieve inflammation. This study evaluated the antioxidant and cytotoxic effect of the ethanolic extract of B. alnifolia and their infusion. It was reported that the ethanol extract contains 4.42 mg GAE/g of dry weight and the infusion contains 35.86 mg GAE/L of total polyphenols. Also, it determined the antioxidant capacity using Ferric reducing antioxidant power (FRAP), Oxygen Radical Absorbance Capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) assay. Moreover, seven metabolites including between phenolic acids and flavonoids were identified for the first time in both extracts of the medicinal plant Baccharis alnifolia. The UHPLC-DAD chromatograms revealed the majority presence of ferulic acid and luteolin, both could be responsible for the antioxidant and cytotoxic activity. Furthermore, we realized a preliminary screening of cytotoxicity in different tumor cell lines, finding that these extracts have cytotoxic potential on kidney cells.

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Inhibition of pro-inflammatory enzymes by medicinal plants from the Argentinean highlands (Puna)

Cited by 34 publications

References 32 publications

In vitro pro-inflammatory enzyme inhibition and anti-oxidant potential of selected Sri Lankan medicinal plants

In vitro pro-inflammatory enzyme inhibition and anti-oxidant potential of selected Sri Lankan medicinal plants

Effect of Wine Wastes Extracts on the Viability and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus Strains

Potential of Baccharis alnifolia Meyen & Walpan (Chilka) from northern Chile used as a medicinal infusion

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