Inhibition Of Rhizoctonia Solani Growth and Its Extracellular Hydrolytic Enzymes by Different Extracts of Cinnamon (Cinnamomum Cassia) and Black Cumin Seeds (Nigella Sativa)

aziz, seham abd el; Ibrahim, Mervat A. R.; Helmy, M M

doi:10.21608/ajs.2022.92048.1410

Cited by 2 publications

(1 citation statement)

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“…Recently, several studies have demonstrated that black cumin seeds have an inhibitory effect on different types of proteases including the phytopathogenic fungi R. solani extracellular protease. It has been reported that black cumin seeds' methanolic extract at 2000 ppm inhibited 74% of R. solani protease activity [15]. Moreover, it has been reported that black cumin seeds' oil inhibited 75.4% and 91.1% of the serine protease elastase type I and type II activity, respectively, and 92.4% of collagenase activity at a concentration of 300 µg/mL [16].…”

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confidence: 98%

Isolation and Identification of Flavonoids from Black Cumin (Nigella sativa) by HPLC-MS and In Silico Molecular Interactions of Their Major Compounds with Fusarium oxysporum Trypsin-like Serine Protease

et al. 2023

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Fusarium oxysporum is one of the most harmful soil-borne pathogens that cause root rot, damping-off, and wilt disease in many plant species. Management of Fusarium oxysporum diseases is often by using many harmful and expensive chemical fungicides which have many harmful effects on the environment and human health. The current study was conducted to identify the chemical constituents of black cumin seeds’ methanolic extract and investigate the ability of the major constituents to inhibit the Fusarium oxysporum trypsin-like serine protease, which play an important role in F. oxysporun pathogenicity. The HPLC-MS analysis of black cumin seeds’ methanolic extract revealed the presence of seven major compounds: amentoflavone, Procyanidin C2, Quercetin3-O-sophoroside-7-O-rhamnoside, 5,7-Dihydroxy-3,4-dimethoxyflavone, Borapetoside A, tetrahydroxy-urs-12-en-28-O-[b-D-glucopyranosyl (1-2)-b-D-glucopyranosyl] ester, and kudzusapongenol A-hexA-pen. The results of molecular docking between these compounds and the active site of Fusarium oxysporium trypsin showed that only four compounds were able to bind to the active site of F. oxysporum trypsin. Amentoflavone, 5,7-Dihydroxy-3,4-dimethoxyflavone, and Quercetin3-O-sophoroside-7-O-rhamnoside have the highest binding energy, −6.4, −6.5, and −6.5 Kcal/mol, respectively. In addition, the results clarify that 5,7-Dihydroxy-3,4-dimethoxyflavone was the only compound to form a hydrogen bond with Asp189 (the residue responsible for substrate specificity). The results of the study strongly indicate that flavonoids of black cumin seeds’ methanolic extract could be used as effective inhibitors for the F. oxysporum trypsin-like serine protease.

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Section: Introductionmentioning

confidence: 98%

Isolation and Identification of Flavonoids from Black Cumin (Nigella sativa) by HPLC-MS and In Silico Molecular Interactions of Their Major Compounds with Fusarium oxysporum Trypsin-like Serine Protease

et al. 2023

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The Emergence of N. sativa L. as a Green Antifungal Agent

Pandey,

Bhargava

2024

MRMC

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Background:: Nigella sativa L. has been widely used in the Unani, Ayurveda, Chinese, and Arabic medicine systems and has a long history of medicinal and folk uses. Several phytoconstituents of the plant are reported to have excellent therapeutic properties. In-vitro and in-vivo studies have revealed that seed oil and thymoquinone have excellent inhibitory efficacy on a wide range of both pathogenic and non-pathogenic fungi. Objective:: The present review aims to undertake a comprehensive and systematic evaluation of the antifungal effects of different phytochemical constituents of black cumin. Method:: An exhaustive database retrieval was conducted on PubMed, Scopus, ISI Web of Science, SciFinder, Google Scholar, and CABI to collect scientific information about the antifungal activity of N. sativa L. with 1990 to 2023 as a reference range using ‘Nigella sativa,’ ‘Nigella oil,’ ‘antifungal uses,’ ‘dermatophytic fungi,’ ‘candidiasis,’ ‘anti-aflatoxin,’ ‘anti-biofilm’ and ‘biological activity’ as the keywords. Results:: Black cumin seeds, as well as the extract of aerial parts, were found to exhibit strong antifungal activity against a wide range of fungi. Among the active compounds, thymoquinone exhibited the most potent antifungal effect. Several recent studies proved that black cumin inhibits biofilm formation and growth. Conclusion:: The review provides an in-depth analysis of the antifungal activity of black cumin. This work emphasizes the need to expand studies on this plant to exploit its antifungal properties for biomedical applications.

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Inhibition Of Rhizoctonia Solani Growth and Its Extracellular Hydrolytic Enzymes by Different Extracts of Cinnamon (Cinnamomum Cassia) and Black Cumin Seeds (Nigella Sativa)

Cited by 2 publications

References 34 publications

Isolation and Identification of Flavonoids from Black Cumin (Nigella sativa) by HPLC-MS and In Silico Molecular Interactions of Their Major Compounds with Fusarium oxysporum Trypsin-like Serine Protease

Isolation and Identification of Flavonoids from Black Cumin (Nigella sativa) by HPLC-MS and In Silico Molecular Interactions of Their Major Compounds with Fusarium oxysporum Trypsin-like Serine Protease

The Emergence of N. sativa L. as a Green Antifungal Agent

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