Inhibition of the Bacterial Surface Protein Anchoring Transpeptidase Sortase by Medicinal Plants

Inhibition of the Bacterial Surface Protein Anchoring Transpeptidase Sortase by Isoquinoline Alkaloids

“…The 1 H and 13 C NMR spectra of berberine chloride were found to be the same as those for berberine chloride isolated from C. chinensis rhizomes. [7][8][9][10][11][12][13] The sortase inhibitory activity was assayed based on the previously described procedure 5) with the following modification. Berberine sulfate (ICN Biomedicals Inc., Asse, Belgium), palmatine chloride (Aldrich Chemical Co., Milwaukee, WI), and -hydrastine (Sigma Chemical Co.) were used as received.…”

“…2,3) Up to date, few inhibitors of this enzyme have been reported, may be due to the fact that the target has only recently been purified and demonstrated as critically important for the life cycle of Gram-positive bacteria. 4) During the course of our research to find potential agents to treat Gram-positive bacteria using an in vitro sortase assay system, 5) methanol extract from the rhizomes of Coptis chinensis showed significant inhibitory activity. Here we describe the isolation, structure elucidation, and biological activity of the active substance.…”

Inhibition of Sortase, a Bacterial Surface Protein Anchoring Transpeptidase, by β-Sitosterol-3-O-glucopyranoside fromFritillaria verticillata

“…The characterization of the isolate from F. verticillata was done by analysis of 1D and 2D NMR data as well as by comparisons of its physical and spectral data such as m.p., UV, IR, 1 H-and 13 C-NMR with those in the literature. [10][11][12] The sortase inhibition assay was based on the previously described procedure 9) with the following modiˆcation. Each of the test samples was dissolved in MeOH and diluted with sterilized distilled water before use.…”

“…During the course of our research toˆnd potential agents to treat Gram-positive using in vitro sortase inhibitory assay systems, 9) hexane and ethyl acetate extract from the bulbs of Fritillaria verticillata had signiˆcant activity. Therefore, each extract of the plant was put through a bioassay-guided chromatographic fractionation procedure.…”

Effects of Liriopis Tuber on 4-HNE-induced Apoptosis in PC-12 Cells

“…麥門冬(Liriopis Tuber, LT)은 백합과(Liliaceae)에 속한 다년생 초본인 맥문동 Liriope platyphylla WANG et TANG 의 뿌리의 팽대부로 養陰潤肺, 淸心除煩, 益胃生津한다 10) . 맥문동에 대한 실험적 연구로 천식 11) , 항박테리아 12) , 당 뇨 [13][14][15][16] 및 학습 기억 17) 등의 연구가 보고되었다.…”