Inhibition of the Urease Reaction by Boric Acid

Mazurkiewicz, Jacek; Bingham, Sheila; Runswick, Shirley A.; Ang, B. C. N.

doi:10.1177/000456329303000224

Cited by 8 publications

(6 citation statements)

References 6 publications

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“…It has, however, been reported that boric acid can interfere with the analysis of urea by methods which utilize urease as a reagent. 3 I have investigated the effect of boric acid on two urease-based assays and confirmed that boric acid interferes with these assays. I report a difference in sensitivity to boric acid between the two methods, and discuss possible reasons for this difference.…”

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confidence: 88%

“…The reactions are well described by a model of competitive inhibition of the urease reaction by boric acid with a K, of about boric acid seems unlikely at the molar concentrations present. In their discussion, Mazurkiewicz et al,3 suggested that a rate assay using a glutamate dehydrogenase auxiliary enzyme system may be more sensitive to boric acid than a manual end-point assay because of an effect of boric acid on the auxiliary enzyme system. This would seem unlikely as the CX7 system, which is the most sensitive to boric acid, does not involve an auxiliary enzyme.…”

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Jones

1997

Ann Clin Biochem

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confidence: 88%

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More on the Inhibition of the Urease Reaction by Boric Acid

Jones

1997

Ann Clin Biochem

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“…Carbonic acid and two molecules of ammonia in aqueous solution are at equilibrium with their respective ions, meaning that the reaction equilibrium is affected by the pH of the solution [13]. There are four main classes of urease inhibitors: hydroxamic acids [14], phosphoroamide compounds [15], boric and boronic acids [16], and heavy metal ions [17]. There is significant research describing the influence of urease inhibitors on the enzymatic decomposition of urea.…”

Section: Enzymatic Decomposition Of Ureamentioning

confidence: 99%

Urea removal from aqueous solutions—a review

2016

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The abundance of urea in the natural environment is dictated by the fact that it is one of the major products of mammalian protein metabolism. Due to the extensive use of urea in many branches of industry, it is produced in large quantities. Urea enters into the environment not only with wastewater from the production plants but also by leaching from the fields, agro-breeding farms, and the effluents from the plants using it as a raw material. There are many methods of urea removal, but most of them are still being developed or are very new. The methods themselves differ in terms of physicochemical nature and technological ingenuity. Many wastewater treatment methods include processes such as hydrolysis, enzymatic hydrolysis, decomposition in the biological bed, decomposition by strong oxidants, adsorption, catalytic decomposition, and electrochemical oxidation. In this work, methods of urea removal from aqueous solutions have been reviewed. Particular attention was paid to electrochemical methods.

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“…Boric acid has been used as a urine preservative for biochemical analysis and as a microbiological for over 20 years and concentrations of up to 20 g/L have been recommended for this purpose [17]. Mazurkiewicz et al [18], have recommended that boric acid should not be added to urine at concentrations above 2 g/L for the analysis of urea by methods employing urease. It should be avoided as a urinary preservative when urea is to be measured by urease based-analyzers [19].…”

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confidence: 99%

Boric acid and boronic acids inhibition of pigeonpea urease

Reddy

Kayastha

2006

Journal of Enzyme Inhibition and Medicinal Chemistry

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Urease from the seeds of pigeonpea was competitively inhibited by boric acid, butylboronic acid, phenylboronic acid, and 4-bromophenylboronic acid; 4-bromophenylboronic acid being the strongest inhibitor, followed by boric acid > butylboronic acid > phenylboronic acid, respectively. Urease inhibition by boric acid is maximal at acidic pH (5.0) and minimal at alkaline pH (10.0), i.e., the trigonal planar B(OH)3 form is a more effective inhibitor than the tetrahedral B(OH)4 -anionic form. Similarly, the anionic form of phenylboronic acid was least inhibiting in nature.

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Inhibition of the Urease Reaction by Boric Acid

Cited by 8 publications

References 6 publications

More on the Inhibition of the Urease Reaction by Boric Acid

More on the Inhibition of the Urease Reaction by Boric Acid

Urea removal from aqueous solutions—a review

Boric acid and boronic acids inhibition of pigeonpea urease

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