Inhibition of vascular smooth muscle cell migration by heparin‐like glycosaminoglycans

Majack, Richard A.; Clowes, Alexander W.

doi:10.1002/jcp.1041180306

Cited by 196 publications

(98 citation statements)

References 22 publications

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“…Perlecan is widely expressed in the basement membranes of adult tissues and in all vascular structures (Couchman, 1987;Murdoch et al, 1994) and has been directly implicated as a potent endothelial cell-derived inhibitor of SMC replication (Benitz et al, 1990). Our data indicate that at least some of the gene-regulating activity of perlecan resides in its heparan sulfate side chains, consistent with a large body of evidence implicating heparin-like molecules in the regulation of various SMC functions (Clowes and Karnovsky, 1977;Castellot et al, 1981;Majack and Bornstein, 1984;Majack and Clowes, 1984;Marcum and Rosenberg, 1984;Majack et al, 1985;Fritze et al, 1985;Benitz et al, 1990;Campbell et al, 1992;Pukac et al, 1992;Au et al, 1993).…”

Section: Role Of Basement Membrane Components Insupporting

confidence: 84%

Perlecan regulates Oct-1 gene expression in vascular smooth muscle cells.

Weiser

Grieshaber

Schwartz

et al. 1997

MBoC

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Vascular smooth muscle cells (SMCs) are very quiescent in the mature vessel and exhibit a remarkable phenotype-dependent diversity in gene expression that may reflect the growth responsiveness of these cells under a variety of normal and pathological conditions. In this report, we describe the expression pattern of Oct-1, a member of a family of transcription factors involved in cell growth processes, in cultured and in in vivo SMCs. Oct-1 mRNA was undetectable in the contractile-state in vivo SMCs; was induced upon disruption of in vivo SMC-extracellular matrix interactions; and was constitutively expressed by cultured SMCs. Oct-1 transcripts were repressed when cultured SMCs were plated on Engelbreth-Holm-Swarm tumor-derived basement membranes (EHS-BM) but were rapidly induced after disruption of SMC-EHS-BM contacts; reexpression was regulated at the transcriptional level. To identify the EHS-BM component involved in the active repression of Oct-1 mRNA expression, SMCs were plated on laminin, type IV collagen, fibronectin, or perlecan matrices. Oct-1 mRNA levels were readily detectable when SMCs were cultured on matrices composed of laminin, type IV collagen, or fibronectin but were repressed when SMCs were cultured on perlecan matrices. Finally, the Oct-1-suppressing activity of EHS-BM was sensitive to heparinase digestion but not to chondroitinase ABC or hyaluronidase digestion, suggesting that the heparan sulfate side chains of perlecan play a biologically important role in negatively regulating the expression of Oct-1 transcripts.

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Section: Role Of Basement Membrane Components Insupporting

confidence: 84%

Perlecan regulates Oct-1 gene expression in vascular smooth muscle cells.

Weiser

Grieshaber

Schwartz

et al. 1997

MBoC

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“…In the first, migration was measured using a monolayer-wounding protocol in which cells migrated from a confluent area into an area that was mechanically denuded of cells. 21 In the second type of assay, collagen-coated transwell tissue culture inserts composed of a polycarbonate membrane containing 8-m pores were used. This assay method determines cell chemotaxis.…”

Section: Migration Assaysmentioning

confidence: 99%

Nitric Oxide and C-Type Atrial Natriuretic Peptide Stimulate Primary Aortic Smooth Muscle Cell Migration via a cGMP-Dependent Mechanism

Brown

Pan

Hassid

1999

Circulation Research

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Abstract-Migration of aortic smooth muscle cells is thought to be of essential importance in vascular restenosis, remodeling, and angiogenesis. Recent studies have shown that NO donors inhibit the migration of subcultured aortic smooth muscle cells. However, there is evidence that NO elicits opposite effects on cell proliferation in primary versus subcultured cells, indicating fundamental differences among different models of aortic smooth muscle cell cultures. The purpose of the current study was to investigate the effect of NO donors on migration of primary cultures of rat aortic smooth muscle cells and to compare and contrast their response with those in subcultured cells. A second purpose was to investigate some of the underlying mechanisms associated with NO-induced effects on cell migration. We report that 2 NO donors, S-nitroso-N-acetylpenicillamine (SNAP) and 2,2-(hydroxynitrosohydrazino)bis-ethanamine, stimulated the migration of primary cells in a wounded-culture model as well as in a transwell migration model. The effect of NO donors was mimicked by 2 cGMP analogues and C-type natriuretic peptide and blocked by a specific inhibitor of guanyl cyclase, 1H-(1,2,4)oxadiazolo[4,3,-a]quinoxalin-1-one, indicating the involvement of cGMP as second messenger. Moreover, neither NO donors nor cGMP analogues altered migration of primary cultures stimulated by either FBS or angiotensin II. In contrast to its effect in primary cultures, SNAP did not alter basal or stimulated migration of subcultured cells, except at a relatively high concentration of 1 mmol/L, at which migration was inhibited. The migration-stimulatory effect of NO donors and cGMP was associated with altered cell morphology and dissociation of actin filaments, consistent with recent studies indicating that cell morphology and cytoskeletal organization influence cell migration. The results suggest the possible involvement of NO-induced cell migration in vascular injury or remodeling, representing conditions in which vascular NO levels would be expected to be elevated. (Circ Res. 1999;84:655-667.)

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“…103 Little is known about the factors that control this process. Recent studies by Majack and Clowes 132 have shown that heparin inhibits arterial smooth muscle cell migration in vitro in a dose-dependent and reversible fashion, but hyaluronate and chondroitin or dermatan sulfate do not. Furthermore, this effect was specific for arterial smooth muscle cells, since similar 29 Shaded areas represent proportion of total radioactivity present in the cell layer.…”

Section: Cell Migrationmentioning

confidence: 99%