Inhibition studies on antibody to poly(L-tyrosyl-L-glutamyl-L-alanylglycyl)glycine-1-14C ethyl ester. Synthesis and immunochemical properties of poly(L-alanyl-L-glutamy1-L-alanylglycyl)glycine-1-14C ethyl ester

Conformation dependency of antibody to poly(L-tyrosyl-L-glutamyl-L-alanylglycyl)glycine-l-14C ethyl ester

“…The bicyclic ring system in 1 appears to be necessary for activity in that cyclopropanecarboxylic acid (5) and cyclohexanecarboxylic acid (6) were both inactive at the highest doses tested. The exo configuration is required in that the corresponding endo isomer (7) and two of its amides, 15 and 16, were inactive while the corresponding exo isomers (1, 17, and 21) were active. Variations in the size of the larger ring (see Table I) resulted in 2 which possessed substantially greater activity than the initial lead (1).…”

Conformation Dependency of Antibodies to Poly(L-tyrosyl-L-glutamyl-L-alanylglycyl)glycine-1-14C Ethyl Ester

“…The polymerization was performed at a reagent concentration of 100 mmoles/l. in the presence of a preformed monomer, since this has been shown t o produce linear high molecular weight polypeptides ( 1 , 2, [5][6][7][8][9][10][11][12]. Following this established procedure, the insoluble polymer, poly(0-benzyl -L -tyrosyl -c -N -benzyloxycarbonyl -L -lysyl -L -alanylglycy1)glycine methyl ester (VI), was prepared, from which the protecting groups were removed by the use of anhydrous hydrogen bromide in t rifluoroacetic acid to yield poly(L-tyrosyl-L-lysyl-L-alanylglycy1)-glycine methyl ester (VII).…”

“…Agostini and Schulman (4) used the so-called "Schulman cell" to investigate ion flux and water migration across biological membranes. Perrin (5) and Augustine and Swarbrick (2) reported using this cell in studies designed to simulate the GI absorption of drugs. In addition to the lipid phase composition, it was shown that stirring rate, the surface to volume ratio of the phases, and temperature also affect the transport rate constants obtained (2).…”

Antigenic determinants of the polypeptide, poly(L-tyrosyl-L-glutamyl-L-alanylglycyl)glycine-1-14C ethyl ester

“…The polymerization was performed in the presence of a preformed monomer since this has been shown to produce linear high molecular weight polypeptides. [1][2][3][4][5][6][7][8][9][10][11]17 Following this established procedure, the insoluble polymer, poly(O-tert-butyl-L-tyrosyl-7-tertbutyl-L-glutamyl-L-alanyl-d-alanyl)glycine methyl ester, was prepared, from which the protecting terf-butyl groups were removed by use of 90% trifluoroacetic acid to yield poly(L-tyrosyl-L-glutamyl-L-alanyl-/3-alanyl)glycine methyl ester (2). After extensive dialysis, the polymer 2 was fractionated by successive diafiltration through Diaflo membranes into four different molecular weight fractions: 5 x 104; 2-5 x 104; 1-2 x 104; and 1 x 104.…”