Inhibitory Activity Goblet Depletion and focal inflammatory Phaleria macrocarpha Leaves Ethanol Extract on Crypta Mouse after Dextran Sodium Sulphate Induction

Maharani, Rianti; Kusmardi,; Elya, Berna

doi:10.20902/ijptr.2019.120106

Cited by 3 publications

(5 citation statements)

References 9 publications

(7 reference statements)

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“…The dried material then undergo maceration using method adapted from the method of Wilson. 14 The fine dried powder (1000g) was soaked with 3 L 70% ethanol solvent. After 24 hours, the extract was filtered through using Whatman filter paper no.1 to take the filtrate.…”

Section: Plant Extract Preparationmentioning

confidence: 99%

“…The extract obtained by maceration produced phenol with a grade of 4.4103% or 44.103 GAE/g and flavonoids with levels of 0.3429% or 3.429 mgQE/g, and have IC 50 219.716 μg/mL (moderate antioxidant intensity). 14…”

Section: Plant Extract Preparationmentioning

confidence: 99%

“…Then, the sections were incubated with anti-Caspase-3 antibodies in phosphate buffer solution (PBS) for 2 hours at room temperature then visualized using 3,3'-diaminobenzidine (DAB) for 10 minutes. 14,15 The sample sections were dipped in a Lilie Mayer haematoxylin solution (counterstain) for 1-2 minutes then rinsed again with water. 16 Next, the sections were dipped in lithium carbonate for 60 seconds then rinsed.…”

Section: Tissue Handling and Immunohistochemistrymentioning

confidence: 99%

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Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction

Kusmardi¹,

Wiyarta²,

Estuningtyas³

et al. 2021

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Ulcerative colitis (UC) is a part of incurable chronic inflammatory disease that has gained importance over the past few decades. A lot of research has been done to find effective treatments for UC, one of which is herbal medicine. Phaleria macrocarpa (PM), an Indonesian native plant, is thought to be an alternative therapy for UC because of its anti-inflammatory properties. Therefore, in this research, Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is used to assess its effect on UC by using Caspase-3 as apoptosis marker. PMLEE was made from dried material of PM that undergo maceration. Animals were separated into six groups: normal, negative control, positive control, and PMLEE groups (100, 200, 300 mg/kgBW). PMLEE was then injected to BALB/c mice that have been induced by dextran sodium sulphate (DSS) for 7 consecutive days. DSS is used to model UC in mice colon tissue. All animals were sacrificed and their colons were collected then stained with anti-Caspase-3. The stained sections were subsequently examined with ImageJ based on color intensity which generated H-Score as the results. Based on H-Score of each group, PMLEE 300mg has significantly upregulate the expression of Caspase-3 compare to the negative control (p=0.015). PMLEE also has a tendency to be dose dependent based on the significant difference between PMLEE doses. Therefore, it concludes that PMLEE is able to upregulate the expression of Caspase-3 in colon cells as in this study it was directly proportional.

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Section: Plant Extract Preparationmentioning

confidence: 99%

Section: Plant Extract Preparationmentioning

confidence: 99%

Section: Tissue Handling and Immunohistochemistrymentioning

confidence: 99%

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Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction

Kusmardi¹,

Wiyarta²,

Estuningtyas³

et al. 2021

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“…Uncontrolled activation of neutrophils with UC causes damage to the colonic epithelial tissue and allows infiltration into the crypts [21]. Showed the antiinflammatory effect of P. macrocarpa leaf extract by suppressing the amount of inflammatory focus in DSS-induced UC [16].…”

Section: Discussionmentioning

confidence: 99%

“…From this study, it was concluded that the P. macrocarpa leaf extract can reduce colonic tissue damage and prevent an increase in the expression of COX-2, NOS and β-catenin [15]. A study by Maharani et al showed that the P. macrocarpa leaf extract with a dose of 100 mg, 200 mg, and 300 mg can inhibit a decrease in the number of goblet cells in the colon [16].…”

Section: Effect Of Administration Of P Macrocarpa Leaf Extract On the Number Of Goblet Cellsmentioning

confidence: 99%

Potential of Ethanol Extract of Mahkota Dewa Leaves (Phaleria Macrocarpa (Schecff.) Boerl.) to Inhibit Inflammation in Mouse Distal Colon Induced by Dextran Sodium Sulfate (Dss) and Azoxymethane (Aom)

et al. 2020

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Objective: To demonstrates the ability of P. macrocarpa leaf extract to reduce inflammation of the distal colon in DSS/AOM-induced mice. Methods: In vivo experimental research using Balb/c mice induced by 0.2 ml azoxymethane (AOM) 0.1% once and 1% dextran sodium sulphate (DSS) for one week; additionally, ethanol extract of P. macrocarpa leaves, 25 mg and 50 mg, and 0.84 mg acetosal were given orally. The mice were sacrificed after 20 w. Histopathological examination (hematoxylin-eosin staining) was conducted by counting the average number of goblet cells per crypt, inflammatory focus and angiogenesis. Results: Ethanol extract of P. macrocarpa leaves was able to prevent the decrease in the number of goblet cells (p<0.05). However, the administration of ethanol P. macrocarpa leaf extract could not reduce focal inflammation and angiogenesis in inflammation of the distal colon. Conclusion: Ethanol extract of the Mahkota Dewa leaves is able to prevent inflammation of the distal colon by preventing the decrease in the number of goblet cells.

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Effect of Omega-3-Rich Fish Oil on TNF-  Expression in Mice's Colonic Tissue Induced with Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS)

Wiyarta

Kusmardi

Midoen

2022

RJPT

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Objective: Colorectal cancer (CC) is one type of cancer with a high incidence worldwide. Many therapeutic techniques have been for CC but have not yet yielded satisfactory results. Fish oil has potential as an alternative therapy for CC through its anti-inflammatory effects. Here, we want to investigate that effect on TNF-α expression using omega-3-rich fish oil (FO). Methods: FO was injected into Swiss mice that have been induced (2% in drinking water) with DSS for seven consecutive days. Animals were separated into six groups: normal, negative control, positive control, solvent control, and FO groups (3 and 6 mg/kg body weight/day). All animals were sacrificed, and the colons were collected then stained with anti-TNF-α. The stained sections were subsequently examined with ImageJ based on colour density. Results: Based on the H-Score of each group, FO 3 mg and 6 mg has significantly decreased the expression of TNF-α compared to the negative control (p=0.001 and p=0.009). Conclusion: FO administration was able to inhibit the expression of TNF-α on mice's colonic tissue induced with AOM and DSS.

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Inhibitory Activity Goblet Depletion and focal inflammatory Phaleria macrocarpha Leaves Ethanol Extract on Crypta Mouse after Dextran Sodium Sulphate Induction

Cited by 3 publications

References 9 publications

Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction

Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction

Potential of Ethanol Extract of Mahkota Dewa Leaves (Phaleria Macrocarpa (Schecff.) Boerl.) to Inhibit Inflammation in Mouse Distal Colon Induced by Dextran Sodium Sulfate (Dss) and Azoxymethane (Aom)

Effect of Omega-3-Rich Fish Oil on TNF-  Expression in Mice's Colonic Tissue Induced with Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS)

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