Inhibitory Effect of Eriodictyol on IgE/Ag-Induced Type I Hypersensitivity

Summary Western diets induce obesity associated with an increased risk of hypercholesterolaemia. Indeed, obesity-induced hypercholesterolaemia is correlated with increased coronary cardiovascular disease (CVD) risk. Male C57BL/6J mice were fed a normal diet, highfat and high-sucrose diet (HF/HS), HF/HS with green tea extract powder diet (HF/HS1GT), HF/HS with eriodictyol diet (HF/HS1Eri), or HF/HS with green tea extract powder and eriodictyol diet (HF/HS1GT1Eri) for 8 wk. Body weight was lower in the HF/HS1GT1Eri group than in the HF/HS group (28.3%, p,0.01). The HF/HS diet elicited an upregulation of total cholesterol levels (263%, p,0.001), and low-density lipoprotein (LDL) levels (289%, p,0.001) were significantly suppressed by the GT1Eri diet. Conversely, no change (p.0.05) was observed in the HF/HS1GT and HF/HS1Eri groups. The HF/HS diet-induced hepatic mRNA increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) was ameliorated (273%) by the oral administration of green tea extract and eriodictyol. Moreover, the GT1Eri diet suppressed HF/HS diet-induced upregulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGCS) (275%, p,0.05). Furthermore, the LDL receptor (LDLR) levels were higher in the HF/HS1GT1Eri group (150%, p,0.05) than in the HF/ HS group. These results suggest that a combination of green tea and eriodictyol decreases cholesterol levels, particularly LDL levels, accompanied by the suppression of HMGCR and HMGCS levels and upregulation of LDLR levels in the liver.

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“…The doses of both eriodictyol (28) and green tea extract (29) in our study were determined as reference.…”

Section: Methodsmentioning

confidence: 99%

The Combination of Green Tea Extract and Eriodictyol Inhibited High-Fat/High-Sucrose Diet-Induced Cholesterol Upregulation Is Accompanied by Suppression of Cholesterol Synthesis Enzymes

Yamashita

Kumazoe

Nakamura

et al. 2016

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…20 Preincubation of mast cells with anti-asthmatic drugs, cedrol, and CR-NLCs showed a dose-dependent inhibition of β-hexosaminidase release from compound 48/80-sensitized mast cells, indicating their mast cell stabilizing activity. Comparative analysis of SIs of the anti-asthmatic drug, cedrol, and CR-NLC-treated mast cells revealed highest SI of NLC-C 2 (29.8) followed by NLC-C 1 (23.6)  cromolyn sodium Figure 6 Penetration of coumarin-6-Nlc (cM-Nlc) in mast cells.…”

mentioning

confidence: 92%

“…Only a fraction of ~250,000 plant species have been studied for antiasthmatic activity till now. [14][15][16][17][18][19][20][21] WHO has reported that 80% people of world population believe in traditional medicine for their health management. 22 In our laboratory, we have screened a vast number of natural extract in the form of steam volatile oil in vitro for their inhibitory effect on mast cell degranulation and anaphylactic shock.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of a new orally active cedrol-loaded nanostructured lipid carrier on compound 48/80-induced mast cell degranulation and anaphylactic shock in mice

Chakraborty¹,

Kar²,

Kumari³

et al. 2017

IJN

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Background Type I hypersensitivity is an allergic reaction characterized by the overactivity of the immune system provoked by normally harmless substances. Glucocorticoids, anti-histamines, or mast cell stabilizers are the choices of treatment for type I hypersensitivity. Even though these drugs have the anti-allergic effect, they can have several side effects in prolong use. Cedrol is the main bioactive compound of Cedrus atlantica with anti-tumor, anti-oxidative, and platelet-activating factor inhibiting properties. Methods In this study, the preparation and anti-anaphylactic effect of cedrol-loaded nanostructured lipid carriers (NLCs) were evaluated. NLCs were prepared using Compritol ® 888 ATO and triolein as lipid phase and vitamin E d -α-tocopherylpolyethyleneglycol 1000 succinate, soya lecithin, and sodium deoxycholate as nanoparticle stabilizers. Results The average diameter of cedrol-NLCs (CR-NLCs) was 71.2 nm (NLC-C 1 ) and 91.93 nm (NLC-C 2 ). The particle had negative zeta potential values of −31.9 mV (NLC-C 1 ) and −44.5 mV (NLC-C 2 ). Type I anaphylactoid reaction in the animal model is significantly reduced by cedrol and cedrol-NLC. This in vivo activity of cedrol resulted that cedrol suppressed compound 48/80-induced peritoneal mast cell degranulation and histamine release from mast cells. Furthermore, compound 48/80-evoked Ca 2+ uptake into mast cells was reduced in a dose-dependent manner by cedrol and cedrol-NLC. Studies confirmed that the inhibition of type I anaphylactoid response in vivo in mice and compound 48/80-induced mast cell activation in vitro are greatly enhanced by the loading of cedrol into the NLCs. The safety of cedrol and CR-NLC was evaluated as selectivity index (SI) with prednisolone and cromolyn sodium as positive control. SI of CR-NLC-C 2 was found to be 11.5-fold greater than both prednisolone and cromolyn sodium. Conclusion Administration of CR-NLC 24 hours before the onset of anaphylaxis can prevent an anaphylactoid reaction. NLCs could be a promising vehicle for the oral delivery of cedrol to protect anaphylactic reactions.

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“…Several research groups have reported that eriodictyol, luteolin, and quercetin inhibited mast cell degranulation (Yoo et al, 2012;Kimata et al, 2000;Matsuda et al, 2002). Herein, we investigated the effect of genistein, daidzein, and equol on histamine release, another chemical mediator, from calcium ionophore-stimulated rat PEC.…”

Section: Resultsmentioning

confidence: 99%

Effects of Soybean Isoflavones on the Release of Chemical Mediators from Rat Peritoneal Exudate Cells by Allergic Reaction in Vitro

Takasugi

Shimada

Yamada

et al. 2014

FSTR

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Soybean isoflavones are expected to reduce the risks of various diseases. In this study, anti-allergic effects of soybean isoflavones such as genistein and daidzein, and equol, a metabolite of daidzein, were investigated by measuring the chemical mediators, leukotriene B 4 (LTB 4 ) and histamine, released from rat peritoneal exudate cells (PEC) in vitro. Genistein and equol significantly suppressed the release of LTB 4 from PEC stimulated by calcium ionophore without cytotoxicity, whereas the inhibitory effect of daidzein was weak. In contrast, they had no effect on the release of histamine from calcium ionophore-stimulated PEC. These data suggest that the soybean isoflavones and metabolite may contribute to allergy symptom relief by inhibiting leukotriene production, but not histamine release.

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Inhibitory Effect of Eriodictyol on IgE/Ag-Induced Type I Hypersensitivity

Cited by 16 publications

References 29 publications

The Combination of Green Tea Extract and Eriodictyol Inhibited High-Fat/High-Sucrose Diet-Induced Cholesterol Upregulation Is Accompanied by Suppression of Cholesterol Synthesis Enzymes

The Combination of Green Tea Extract and Eriodictyol Inhibited High-Fat/High-Sucrose Diet-Induced Cholesterol Upregulation Is Accompanied by Suppression of Cholesterol Synthesis Enzymes

Inhibitory effect of a new orally active cedrol-loaded nanostructured lipid carrier on compound 48/80-induced mast cell degranulation and anaphylactic shock in mice

Effects of Soybean Isoflavones on the Release of Chemical Mediators from Rat Peritoneal Exudate Cells by Allergic Reaction in Vitro

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