Inhibitory effect of furosemide on activation of human peripheral blood polymorphonuclear leukocytes stimulated with n-formyl-methionyl-leucyl-phenylalanine

Białasiewicz, Piotr; Włodarczyk, Anna; Dudkiewicz, B; Nowak, Dariusz

doi:10.1016/j.intimp.2004.01.024

Cited by 5 publications

(5 citation statements)

References 35 publications

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“…In vitro furosemide decreased ROS production by PMNs stimulated with fMLP, but only at concentrations at least 100-times higher than that observed in plasma after intravenous drug infusion [3]. Oral administration of enalapril in patients with moderate heart failure as well as in vitro cell incubation with the drug had no influence on ROS production by human PMNs [6,49].…”

Section: Discussionmentioning

confidence: 99%

Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

Rysz

Potargowicz

Banach

et al. 2006

Arch. Immunol. Ther. Exp.

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Section: Discussionmentioning

confidence: 99%

Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

Rysz

Potargowicz

Banach

et al. 2006

Arch. Immunol. Ther. Exp.

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“…PMNs were saturated with Fura-2AM as described in our previous study (Bialasiewicz et al, 2004) ] i response, part of the cells was incubated (30 min at 37 C in darkness) with selected polyphenols at a concentration of 5 mM, or control solvent (DMSO dissolved in 0.9% NaCl). Then PMNs were transferred into a microcuvette of a fluorescence spectrometer.…”

Section: Measurement Of Cytosolic-free Calciummentioning

confidence: 99%

“…Polyphenol chemoattractant activity for human PMNs was assessed according to the procedure described in our previous study (Bialasiewicz et al, 2004), in 48-well microchemotaxis plates (Neuro Probe, Gaithersburg, MD) with wells separated by a polycarbonate membrane with a pore size of 5 mm (Harvath et al, 1980). About 29 mL samples of a 5 mM polyphenol solution, a 0.1 mM fMLP solution in PBS (known chemoattractant-positive control) and PBS alone (negative control) were placed in the bottom chambers of wells where appropriate.…”

Section: Chemotaxis Assaymentioning

confidence: 99%

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Nowak

Zasowska-Nowak

Białasiewicz

et al. 2015

Pharmaceutical Biology

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Context: Polymorphonuclear leukocytes (PMNs) produce oxidants, contributing to systemic oxidative stress. Diets rich in plant polyphenols seem to decrease the risk of oxidative stressinduced disorders including cardiovascular disease. Objective: The objective of this study was to examine the in vitro effect of each of the 14 polyphenols on PMNs chemotaxis, intracellular calcium response, oxidants production. Materials and methods: Blood samples and PMNs suspensions were obtained from 60 healthy non-smoking donors and incubated with a selected polyphenol (0.5-10 mM) or a control solvent. We assessed resting and fMLP-dependent changes of intracellular calcium concentration ([Ca 2+ ] i ) in PMNs with the Fura-2AM method and measured fMLP-induced luminol enhanced whole blood chemiluminescence (fMLP-LBCL). Polyphenol chemoattractant activity for PMNs was tested with Boyden chambers. Results: Polyphenols had no effect on resting [Ca 2+ ] i . Unaffected by other compounds, fMLPdependent increase of [Ca 2+ ] i was inhibited by quercetin and catechol (5 mM) by 32 ± 14 and 12 ± 10% (p50.04), respectively. Seven of the 14 tested substances (5 mM) influenced fMLP-LBCL by decreasing it. Catechol, quercetin, and gallic acid acted most potently reducing fMLP-LBCL by 49 ± 5, 42 ± 15, and 28 ± 18% (p50.05), respectively. 3,4-Dihydroxyhydrocinnamic, 3,4-dihydroxyphenylacetic, 4-hydroxybenzoic acid, and catechin (5 mM) revealed distinct (p50.02) chemoattractant activity with a chemotactic index of 1.9 ± 0.8, 1.8 ± 0.7, 1.6 ± 0.6, 1.4 ± 0.2, respectively. Conclusion and discussion: Catechol, quercetin, and gallic acid at concentrations commensurate in human plasma strongly suppressed the oxidative response of PMNs. Regarding quercetin and catechol, this could result from an inhibition of [Ca 2+ ] i response.

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“…These include anion exchangers (Simchowitz and De Weer, 1986), Na + /K + /2Cl - co-transporters (Bialasiewicz et al, 2004), and calcium- and swell-activated channels (Krause and Welsh, 1990; Schumann et al, 1993; Stoddard et al, 1993). Amongst the diffusive pathways available to Cl - in neutrophils; the evidence of a large Cl - efflux (Shimizu et al, 1993; Busetto et al, 2007), the swelling of the phagocytic vacuole (Reeves et al, 2002; Levine et al, 2015), and the recent molecular identification of LRRC8A as an indispensable component of the volume-regulated anion channel (VRAC) or volume-sensitive outwardly rectifying (VSOR) current (Qiu et al, 2014; Voss et al, 2014) prompted us to consider this channel as a possible participant in compensation of the current generated by the oxidase.…”

Section: Introductionmentioning

confidence: 99%

The LRRC8A Mediated “Swell Activated” Chloride Conductance Is Dispensable for Vacuolar Homeostasis in Neutrophils

et al. 2017

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The dialysis of human and mouse neutrophils in patch clamp experiments in the conventional whole-cell mode induces the emergence of a chloride (Cl-) current that appeared to be primarily regulated by cytoplasmic ionic strength. The characteristics of this current resembled that of the classical, and ubiquitous volume-sensitive outwardly rectifying Cl- current: strong outward rectification, selectivity sequence of the Eisenman1 type, insensitivity to external pH and strong inhibition by tamoxifen, DCPIB and WW781. We show that this current is essentially supported by the leucine rich repeat containing 8 A (LRRC8A); the naturally occurring LRRC8A truncation mutant in ebo/ebo mice drastically reduced Cl- conductance in neutrophils. Remarkably, the residual component presents a distinct pharmacology, but appears equally potentiated by reduced ionic strength. We have investigated the role of the LRRC8A-supported current in the ionic homeostasis of the phagosomal compartment. The vacuolar pH, measured using SNARF-1 labeled Candida albicans, normally rises because of NADPH oxidase activity, and this elevation is blocked by certain Cl- channel inhibitors. However, the pH rise remains intact in neutrophils from the ebo/ebo mice which also demonstrate preserved phagocytic and respiratory burst capacities and normal-sized vacuoles. Thus, the LRRC8A-dependent conductance of neutrophils largely accounts for their “swell activated” Cl- current, but is not required for homeostasis of the phagosomal killing compartment.

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Inhibitory effect of furosemide on activation of human peripheral blood polymorphonuclear leukocytes stimulated with n-formyl-methionyl-leucyl-phenylalanine

Cited by 5 publications

References 35 publications

Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

The LRRC8A Mediated “Swell Activated” Chloride Conductance Is Dispensable for Vacuolar Homeostasis in Neutrophils

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