Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation embryos cultured in vitro

Fabian, Denis; Il’ková, Gabika; Rehák, P; Czikková, S.; Baran, Vladimı́r; Koppel, Juraj

doi:10.1016/s0093-691x(03)00254-1

Cited by 63 publications

(44 citation statements)

References 31 publications

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“…2), although IGF-I did not significantly influence embryo development (Table 1). These results were consistent with a previous study [19], which suggested that the addition of IGF-I to the culture medium decreased the percentage of apoptotic cells in the presence of an apoptosis inductor, but that IGF-I addition had no significant influence on embryonic development.…”

Section: Discussionsupporting

confidence: 93%

“…In embryogenesis, several reports indicated that IGF-I can promote preimplantation embryo development and influence cell numbers [14][15][16][17]. Moreover, a number of studies have demonstrated that IGF-I is a powerful inhibitor of apoptosis through the activation of its receptor [14,[17][18][19]. However, the role of IGF-I in HIF-1α expression in embryogenesis has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

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Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Yoon

Juhn

et al. 2012

J Assist Reprod Genet

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Purpose Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O 2 in both the absence and presence of IGF-I. Results The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O 2 exhibited significantly higher total cell numbers (83.4±18.1) and lower apoptotic index (3.7±1.5) than those cultured under 20 % O 2 (67.4±15.6) (6.9±3.5) (P<0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-apoptotic effect, instead of increasing HIF-1α protein expression.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Yoon

Juhn

et al. 2012

J Assist Reprod Genet

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“…Their expression has been reported in preimplanted embryos in cattle [27], and they are generally added to the basal culture medium to increase cell proliferation and stimulate specific cell functions in vitro [28]. The beneficial effects of various GFs and CYKs in embryo culture media have been reported in different species [4,8,9,29,30].…”

Section: Discussionmentioning

confidence: 99%

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

et al. 2015

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a b s t r a c tThe aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-b1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the best combination of GFs-CYKs þ RA þ HA for optimal embryonic development. Next, we compared development rates and embryo quality (inner cell mass [ICM]-to-total cell number [TCN] ratio), and postthaw survival and hatching rates using this synthetic medium (T1) and a control medium: synthetic oviduct fluid þ BSA þ ITS (insulin, transferrin, and selenium). The blastocyst rates were significantly higher with T1 than those with the control at 7 and 8 days after fertilization. There was no significant difference in TCN or the ICM/TCN ratio between the two treatments. Survival and hatching rates 48 hours after thawing were similar for both treatments. Finally, nine embryo transfers were conducted using fresh and previously frozen Day-7 blastocysts to evaluate the in vivo viability of embryos produced in this synthetic medium; four gestations were obtained from six fresh embryos and one gestation from three frozen embryos. In conclusion, the fetal calf serum and BSA-free medium, supplemented with GFs-CYKs þ RA þ HA, improved embryo development and gave comparable ICM/TCN ratios and postthaw survival rates to the control with BSA. Fresh and frozen embryos produced in this medium are viable for embryo transfer. This fully synthetic method of embryo culture is a useful means of reducing the risk of disease transmission via embryo transfer.

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“…According to their morphology (Hoechst), PI positivity/negativity and CA positivity/negativity, cells were classified into three groups (Fabian et al 2004): normal cells (Hoechst normal PI -CA+; containing oval nuclei without morphological changes, able to exclude PI, with positive CA staining in cytoplasm); apoptotic cells (Hoechst damaged PI ± CA+; containing nuclei with typical fragmented or condensed morphology, PI negative in early stages, PI positive in the final stage of apoptotic process, usually with positive CA staining in cytoplasm); necrotic cells (Hoechst normal/damaged PI + CA-; mostly without specific morphological changes, always PI positive and CA negative).…”

Section: Morphological Triple Stainingmentioning

confidence: 99%

“…Occasional mitotic configurations were also classified as normal nuclei (Fabian et al 2004). The percentage of dead cells represents the proportion of both apoptotic and necrotic cells relative to total embryo cell number.…”

Section: Morphological Triple Stainingmentioning

confidence: 99%

Effects of Fungicide Euparen Multi (Tolylfluanid) on Development of Preimplantation Embryos in Mouse

et al. 2007

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The effect of the fungicide Euparen Multi (containing 50% tolylfluanid) on the development of mouse preimplantation embryos was evaluated. Euparen Multi was daily administered per os to female mice (ICR strain) at four different doses of 118, 294, 588 and 1177 mg/kg b.m., beginning on day 1 of pregnancy. Embryos obtained on day 4 of pregnancy were stained by morphological triple staining (Hoechst 33342, propidium iodide, Calcein AM), and the number of nuclei, blastocyst formation, distribution of embryos according to the nucleus number and cell death incidence were determined. Embryos in the experimental groups (except for the lowest dose 118 mg/kg b.m.) showed a highly significant dose-dependent reduction in total cell numbers corresponding to the lower proportion of blastocysts. The occurrence of cell death was significantly increased in all experimental groups, indicating that Euparen Multi is able to cause cell death at relatively low doses. Our data demonstrate that Euparen Multi could induce significant alterations in the preimplantation embryo development.

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Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation embryos cultured in vitro

Cited by 63 publications

References 31 publications

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

Effects of Fungicide Euparen Multi (Tolylfluanid) on Development of Preimplantation Embryos in Mouse

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