Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses

Abstract

Saponin components are known to be pharmaceutically, cosmetically and nutraceutically valuable principles found in various herbal medicine. In this study, we evaluated the inhibitory role of saponin fraction (SF), prepared from C. lanceolata, an ethnopharmacologically famous plant, on various inflammatory responses managed by monocytes, macrophages, lymphocytes and mast cells. SF clearly suppressed the release of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha, but not prostaglandin E(2) (PGE(2)). While this fraction did not scavenge the reactivity of SNP-induced radicals in RAW264. 7 cells, it negatively modulated the phagocytic uptake of macrophages treated with FITC-dextran. Interestingly, SF completely diminished cell-cell adhesion events induced by both CD29 and CD43, but not cell-fibronectin adhesion. Concanavalin (Con) A [as well phytohemaglutinin A (PHA)]-induced proliferation of splenic lymphocytes as well as interferon (IFN)-gamma production were also clearly suppressed by SF treatment. Finally, SF also significantly blocked the degranulation process of mast cell line RBL-2H3 cell as assessed by DNP-BSA-induced beta-hexosaminidase activity. The anti-inflammatory activities of SF on NO production seemed to be due to inhibition of nuclear factor (NF)-kappaB activation signaling, since it blocked the phosphorylation of inhibitor of kappaB (IkappaB)alpha as well as inducible NO synthase (iNOS) expression. Therefore, these results suggest that SF may be considered as a promising herbal medicine with potent anti-inflammatory actions.

Neuroprotective Effect of Steamed and Fermented Codonopsis lanceolata

“…This plant is widely used to treat several inflammatory diseases, such as asthma, tonsillitis, and pharyngitis (Yongxu and Jicheng, 2008; Byeon et al ., 2009). The compounds from this plant are saponins, alkaloids, tannins, steroids, and polysaccharides (Ushijima et al ., 2008).…”

Inhibition of 2,4-dinitrofluorobenzene-induced atopic dermatitis by topical application of the butanol extract of Cordyceps bassiana in NC/Nga mice

“…2B and C), suggesting that the activation and migration of mast cells may be a target immunopharmacology of CBBF. To show whether CBBF directly modulated histamine release from activated mast cells, we treated RBL-2H3 cells with DNP-labeled IgE (Byeon et al, 2009), and their degranulation events were continuously followed. As Fig.…”

“…The RBL-2H3 cells were grown in DMEM supplemented with 10% FBS in a 5%-CO 2 -containing atmosphere as described previously (Byeon et al, 2009). RBL-2H3 cells (2 × 10 5 cells/well) pretreated with CBBF or azelastine (Sigma, St Louis, MO) were sensitized with 1 g/ml anti-DNP IgE for 60 min at 37 • C. IgE-sensitized cells were washed with PBS.…”

Inhibitory Effects of Total Saponin Korean Red Ginseng on Thromboxane A2Production and P-Selectin ExpressionviaSuppressing Mitogen-Activated Protein Kinases

“…In real, many studies about anti-inflammatory activity by KRG-TS have been reported (Byeon et al, 2009;Park and Cho, 2009;Lee et al, 2014).…”