Inhibitory Effects of Chios Mastic Gum on Gastric Acid Secretion by Histamine-Related Pathway in a Rat Model and Primary Parietal Cells

et al. 2022

Nutrients

The present research purposed to examine the gastro-protective effect of Glycine max (L.) Merr. fermented using Lactobacillus delbrueckii ssp. delbrueckii Rosell-187 (Gastro-AD®) on ethanol/HCl-induced gastric damage, specifically on gastric acid secretion. After oral supplementation of Gastro-AD® to Sprague–Dawley (SD) rats with ethanol/HCl-induced gastric damage, we determined that Gastro-AD® attenuated the gastric mucosal lesion, hemorrhage and gastric acid secretion induced by ethanol/HCl. In addition, we observed that the Gastro-AD® treatment increased the serum prostaglandin E2 level and decreased the levels of gastric acid secretion-related receptors in both gastric tissues and primary gastric parietal cells. Furthermore, it decreased the levels of inflammatory factors, including serum histamine and expression of p-IκB, p-p65, iNOS and COX-2 and the activity of apoptotic signaling pathways, including those involving p-JNK, Bcl2/Bax, Fas, FADD, caspase-8 and caspase-3, in the stomach of the ethanol/HCl-treated rats. Thus, we suggest that Gastro-AD® supplementation may reduce ethanol/HCl-induced gastric acid secretion and prevent gastric injury.

“…The methods for H&E staining of stomach tissues and the histopathological score index of gastric inflammation were described in a previous study [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…The methods for isolation and culture of the primary gastric parietal cell were described in a previous study [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Gastro-Protective Effect of Fermented Soybean (Glycine max (L.) Merr.) in a Rat Model of Ethanol/HCl-Induced Gastric Injury

Lee

et al. 2022

Nutrients

“…Among these, the EtOH-induced model is most widely used to investigate the efficacy of potential drugs because it is independent of gastric acid secretion and resembles acute gastric ulcers in humans [ 9 ]. Furthermore, several strains of rodents including SD rats, Wistar rats, C57BL/6 mice, ICR mice and BALB/c mice have been utilized to conduct most experiments in studies for gastric ulcer, although ICR mice are most commonly used for these studies [ 2 5 10 11 12 ]. However, most used only one strain of one animal species to produce animal models for gastric ulcer and evaluate the anti-gastric ulcer activity of some compounds and extracts.…”

mentioning

confidence: 99%

Comparison of the response using ICR mice derived from three different sources to ethanol/hydrochloric acid-induced gastric injury

Song

et al. 2016

Lab Anim Res

Animal models for gastric ulcers produced by physical, pharmacological and surgical methods have been widely employed to evaluate therapeutic drugs and investigate the mechanism of action of this disease. ICR mice were selected to produce this model, even though several mice and rats have been widely used in studies of gastric ulcers. To compare the responses of ICR mice obtained from three different sources to gastric ulcer inducers, alterations in gastric injury, histopathological structure, and inflammation were measured in Korl:ICR (Korea NIFDS source), A:ICR (USA source) and B:ICR (Japan source) treated with three concentrations of ethanol (EtOH) (50, 70, and 90%) in 150 mM hydrochloric acid (HCl) solution. Firstly, the stomach lesion index gradually increased as the EtOH concentration increased in three ICR groups. Moreover, a significant increase in the level of mucosal injury, edema and the number of inflammatory cells was similarly detected in the EtOH/HCl treated group compared with the vehicle treated group in three ICR groups. Furthermore, the number of infiltrated mast cells and IL-1β expression were very similar in the ICR group derived from three different sources, although some differences in IL-1β expression were detected. Especially, the level of IL-1β mRNA in 50 and 90EtOH/HCl treated group was higher in Korl:ICR and A:ICR than B:ICR. Overall, the results of this study suggest that Korl:ICR, A:ICR and B:ICR derived from different sources have an overall similar response to gastric ulcer induced by EtOH/HCl administration, although there were some differences in the magnitude of their responses.

“…The stimulated gastric acid secretion increases internal pH and gastric mucosal damage (Nam et al. 2014). The gastritis symptoms can progress further to damage of submucosa or muscular layers in the stomach wall, which is called a gastric ulcer.…”

Section: Discussionmentioning

confidence: 99%

“…The gastric juice was centrifuged at 3,000 rpm for 20 min and the total acidity was assayed (Nam et al. 2014). Briefly, 0.5% dimethylaminobenzene alcohol solution and 1% phenolphthalein alcohol solution were added to the gastric juice supernatant.…”

Section: Methodsmentioning

confidence: 99%

Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models

Kwon

Baek

et al. 2019

Pharmaceutical Biology

Context: HemoHIM is a medicinal herbal preparation of Angelica gigas Nakai (Apiaceae), Cnidium officinale Makino (Umbelliferae), and Paeonia japonica Miyabe (Paeoniaceae) developed for immune regulation. HemoHIM has been investigated for its ability to enhance tissue self-renewal and stimulate immune systems. To date, studies on the protective effects of HemoHIM against gastritis and gastric ulcers have not been conducted. Objective: The protective effects of HemoHIM using models of indomethacin and ethanol/hydrochloric acid (EtOH/HCl)-induced gastric mucosal injury were investigated. Materials and methods: Rats were divided into five groups ( n = 10): control, indomethacin, or EtOH/HCl groups, HemoHIM 250, 500 mg kg −1 , and cimetidine 100 mg kg −1 , respectively. Indomethacin (80 mg kg −1 ) and 60% EtOH/150 mM HCl were administered orally 1 h after the administration of samples and rats were anesthetized 3 h after induction. The lesion area (%), inhibition ratio (%), and total acidity were investigated, and tissues were histopathologically analyzed using hematoxylin and-eosin (H&E) staining. Results: HemoHIM significantly reduced gastric injury in indomethacin-induced model (250 and 500 mg kg −1 ; 64.30% and 67.75%, p < 0.001) compared to indomethacin group. In the EtOH/HCl-induced model, HemoHIM reduced gastric lesion (250 and 500 mg kg −1 ; 61.05% and 73.37%, p < 0.001) and gastric acidity (250 and 500 mg kg −1 ; 37.80 and 45.20 meq L −1 , p < 0.001) compared to EtOH/HCl group. H&E staining of the gastric mucosa showed decreased erosion and hemorrhage in HemoHIM group compared to EtOH/HCl group. Discussion and conclusions: Based on the results, HemoHIM is potential candidate for the treatment of gastritis and gastric ulcers.