Inhibitory effects of NADH/NADPH in S9 mix on photo-mutagenicity of thiabendazole following UVA-irradiation in E. coli

Watanabe-Akanuma, Mie; Ohta, Toshihiro

doi:10.3123/jems.27.7

Cited by 2 publications

(1 citation statement)

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“…We previously reported that TBZ shows potent photomutagenicity following UVA irradiation (320-400 nm) for 10 min at a dose rate of 2.5 J/m 2 /sec in Escherichia coli strain WP2uvrA/pKM101 and S. typhimurium strains TA97, TA104, and TA100 (1)(2)(3). Structurally related compounds containing imidazole or thiazole, such as benomyl, mefenacet, tricyclazole, and imazalil are widely used in agriculture as pesticides.…”

Section: Thiabendazolementioning

confidence: 99%

Analysis of Photomutagenicity of Thiabendazole with UVA Irradiation: Absence of 8-Hydroxyguanosine Formation

Watanabe-Akanuma

Inaba

Ohta

2006

Genes and Environment

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We recently reported the photomutagenic property of thiabendazole [2-(4-thiazolyl)-1H-benzimidazole, TBZ], a post harvest fungicide, in bacterial and mammalian cells. In the present study we investigated its photomutagenic potential along with several of its structurally-related pesticides including benomyl, mefenacet, tricyclazole, and imazalil. None of the compounds except TBZ showed photomutagenicity with UVA-irradiation in S. typhimurium TA100, TA98, or E. coli WP2uvrA/pKM101. Since TBZ consists of thiazole and benzimidazole rings, we then investigated photomutagenic potential of thiazole, benzimidazole, and 2-phenylbenzimidazole. Only 2-phenylbenzimidazole showed very weak photomutagenicity with UVA-irradiation. These results indicate both benzimidazole ring and thiazole ring are necessary for UVA-activation of TBZ. We measured 8-hydroxydeoxyguanosine (8-OHdG) in salmon sperm DNA exposed to TBZ and UVA by HPLC-ECD, and found that 8-OHdG was not responsible for the photomutagenesis of TBZ.

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Section: Thiabendazolementioning

confidence: 99%