Initial stages of development of the submandibular gland (human embryos at 5.5–8 weeks of development)

Quirós‐Terrón, Luis; Arráez-Aybar, Luis Alfonso; Murillo-González, Jorge A.; de-la-Cuadra-Blanco, Crótida; Martínez‐Álvarez, María‐Concepción; Sanz-Casado, J. V.; Mérida‐Velasco, José Ramón

doi:10.1111/joa.12955

Cited by 15 publications

(17 citation statements)

References 31 publications

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“…All these stages occurred prehatching except the last one occurred post-hatching. Similar results were observed in human 33,34 , in pig 35 , in rat 36 in quail 37 and in duck 38 . The pattern of the development of the lingual gland in birds is like that described for mammals but the terminal buds are formed at the same time as the lumen of the glands 38 .…”

Section: Discussionsupporting

confidence: 86%

Developmental morphological analyses on the preglottal salivary gland in Japanese quails (Coturnix japonica)

Khalifa

Abd-Elkareem

Gaber

et al. 2021

Microscopy Res & Technique

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To understand the development of the mucous preglottal salivary gland in Coturnix japonica (Japanese quail), morphological and histochemical studies were performed on 20 healthy Japanese quail embryos (aging from 10 th to 17 th incubation days) and 25 healthy quail chicks (aging from 0 th to 60 th days). The primordia of preglottal salivary gland were observed as an epithelial bud at the early embryonic stage, which then elongated and differentiated into secretory units by the end of this stage. In Japanese quails, the preglottal salivary gland was a mucous polystomatic tubuloalveolar unpaired gland composed of two lateral portions and a middle one embedded into the submucosa of the lingual root. The gland openings accompanied taste pore (8.17 μm) of taste buds associated salivary glands type; some skeletal muscle fibers embedded among secretory lobules extended from muscle cricohyoideus at 14 th day-old quail chick. Also, both herbts corpuscles and secretory motor plexus could be detected among secretory lobules. Based on our investigations, the development of the preglottal salivary gland could clearly be distinguished in the embryonic stage into pre bud and bud stages at 10 th day old, cord and branching stages ended by cavitation at 11 th day old, canalization stage at 13 th day old, lobulation and secretory stages by the 17 th day old. This mucous secretion showed different histochemical reactions ended with highly alcinophilic mucous indicated highly sialomucin (acidic) content. Myoepithelial cells could be demonstrated at a 17-day old quail embryo and thereafter surrounded the secretory endpieces of the preglottal salivary gland.

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Section: Discussionsupporting

confidence: 86%

Developmental morphological analyses on the preglottal salivary gland in Japanese quails (Coturnix japonica)

Khalifa

Abd-Elkareem

Gaber

et al. 2021

Microscopy Res & Technique

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“…The first appearance of parasympathetic nerves in mouse SGs is during the initial bud stage at E12.5, as the PSG forms near the epithelial stalk of the budding gland (Knox et al 2010). It has been recently reported that the PSG in human embryos initiates at Carnegie stage 16 (CS16), with a robust PSG evident at CS17, when the submandibular gland has started to initiate (Quirós-Terrón et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Dual Sympathetic Input into Developing Salivary Glands

2019

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Neuronal signaling is known to be required for salivary gland development, with parasympathetic nerves interacting with the surrounding tissues from early stages to maintain a progenitor cell population and control morphogenesis. In contrast, postganglionic sympathetic nerves arrive late in salivary gland development to perform a secretory function; however, no previous report has shown their role during development. Here, we show that a subset of neuronal cells within the parasympathetic submandibular ganglion (PSG) express the catecholaminergic marker tyrosine hydroxylase (TH) in developing murine and human submandibular glands. This sympathetic phenotype coincided with the expression of transcription factor Hand2 within the PSG from the bud stage (E12.5) of mouse embryonic salivary gland development. Hand2 was previously associated with the decision of neural crest cells to become sympathetic in other systems, suggesting a role in controlling neuronal fate in the salivary gland. The PSG therefore provides a population of TH-expressing neurons prior to the arrival of the postganglionic sympathetic axons from the superior cervical ganglion at E15.5. In culture, in the absence of nerves from the superior cervical ganglion, these PSG-derived TH neurons were clearly evident forming a network around the gland. Chemical ablation of dopamine receptors in explant culture with the neurotoxin 6-hydroxydopamine at early stages of gland development resulted in specific loss of the TH-positive neurons from the PSG, and subsequent branching was inhibited. Taken altogether, these results highlight for the first time the detailed developmental time course of TH-expressing neurons during murine salivary gland development and suggest a role for these neurons in branching morphogenesis.

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“…These age groups represented the following developmental stages for tooth differentiation: the cap stage (9-13gw), the early bell stage (14-16gw), and the late bell stage (17-22gw) (Figure 1A–1E) (Nanci and TenCate, 2018; Nelson, 2020). We also collected submandibular salivary glands (SMSG) from three matched timepoints (12-13gw, 14-16gw, 17-19gw) that cover the pseudo-glandular and canalicular stages for salivary gland development (Quirós-Terrón et al, 2019) (Figure 1A).…”

Section: Resultsmentioning

confidence: 99%

Human iPSC Derived Enamel Organoid Guided by Single-Cell Atlas of Human Tooth Development

Alghadeer

Ehnes

Zhao

et al. 2022

Preprint

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Tooth enamel secreted by ameloblasts is the hardest material in the human body, acting as a shield protecting the teeth. However, the enamel is gradually damaged or partially lost in over 90% of adults and cannot be regenerated due to a lack of ameloblasts in erupted teeth. Here we use sci-RNA-seq to establish a spatiotemporal single-cell atlas for the developing human tooth and identify regulatory mechanisms controlling the differentiation process of human ameloblasts. We reveal key signaling pathways involved between the support cells and ameloblasts during fetal development and recapitulate those findings in a novel human ameloblast in vitro differentiation from iPSCs. We furthermore develop a mineralizing enamel organ-like 3D organoid system. These studies pave the way for future regenerative dentistry and therapies toward genetic diseases affecting enamel formation.

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Initial stages of development of the submandibular gland (human embryos at 5.5–8 weeks of development)

Cited by 15 publications

References 31 publications

Developmental morphological analyses on the preglottal salivary gland in Japanese quails (Coturnix japonica)

Developmental morphological analyses on the preglottal salivary gland in Japanese quails (Coturnix japonica)

Dual Sympathetic Input into Developing Salivary Glands

Human iPSC Derived Enamel Organoid Guided by Single-Cell Atlas of Human Tooth Development

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