Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection

Zoonoses and animal diseases threaten human health and livestock biosecurity and productivity. Currently, laboratory confirmation of animal disease outbreaks requires centralized laboratories and trained personnel; it is expensive and time-consuming, and it often does not coincide with the onset or progress of diseases. Point-of-care (POC) diagnostics are rapid, simple, and cost-effective devices and tests, that can be directly applied on field for the detection of animal pathogens. The development of POC diagnostics for use in human medicine has displayed remarkable progress. Nevertheless, animal POC testing has not yet unfolded its full potential. POC devices and tests for animal diseases face many challenges, such as insufficient validation, simplicity, and portability. Emerging technologies and advanced materials are expected to overcome some of these challenges and could popularize animal POC testing. This review aims to: (i) present the main concepts and formats of POC devices and tests, such as lateral flow assays and lab-on-chip devices; (ii) summarize the mode of operation and recent advances in biosensor and POC devices for the detection of farm animal diseases; (iii) present some of the regulatory aspects of POC commercialization in the EU, USA, and Japan; and (iv) summarize the challenges and future perspectives of animal POC testing.

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“…The assay was completed within 5 min and showed a sensitivity of 73% and a specificity of 100% [97].…”

Section: Magnetic Biosensorsmentioning

confidence: 97%

Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices

2022

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“…Magnetic nanoparticles (MNPs) have emerged as promising candidates to develop biosensors for the detection of the deadly respiratory viral infections [ Islam and Ahsan, 2020 ; Barnett et al, 2020 ]. Using MNPs different biosensing techniques have been developed such as magnetic resonance (NMR/MRI) [ Perez et al, 2003 ], fluorescence [ Zheng et al, 2020 ], electrochemical [ Khan et al, 2020 ], and rolling circle amplification [ Tian et al, 2020a ; Tian et al, 2020b ].…”

Section: Biosensors For Covid-19 Diagnosismentioning

confidence: 99%

Prospects of nanomaterials-enabled biosensors for COVID-19 detection

Srivastava

Mishra

et al. 2021

Science of The Total Environment

133

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We are currently facing the COVID-19 pandemic which is the consequence of severe acute respiratory syndrome coronavirus (SARS-CoV-2). Since no specific vaccines or drugs have been developed till date for the treatment of SARS-CoV-2 infection, early diagnosis is essential to further combat this pandemic. In this context, the reliable, rapid, and low-cost technique for SARS-CoV-2 diagnosis is the foremost priority. At present reverse transcription polymerase chain reaction (RT-PCR) is the reference technique presently being used for the detection of SARS-CoV-2 infection. However, in a number of cases, false results have been noticed in COVID-19 diagnosis. To develop advanced techniques, researchers are continuously working and in the series of constant efforts, nanomaterials-enabled biosensing approaches can be a hope to offer novel techniques that may perhaps meet the current demand of fast and early diagnosis of COVID-19 cases. This paper provides an overview of the COVID-19 pandemic and nanomaterials-enabled biosensing approaches that have been recently reported for the diagnosis of SARS-CoV-2. Though limited studies on the development of nanomaterials enabled biosensing techniques for the diagnosis of SARS-CoV-2 have been reported, this review summarizes nanomaterials mediated improved biosensing strategies and the possible mechanisms that may be responsible for the diagnosis of the COVID-19 disease. It is reviewed that nanomaterials e.g. gold nanostructures, lanthanide-doped polysterene nanoparticles (NPs), graphene and iron oxide NPs can be potentially used to develop advanced techniques offered by colorimetric, amperometric, impedimetric, fluorescence, and optomagnetic based biosensing of SARS-CoV-2. Finally, critical issues that are likely to accelerate the development of nanomaterials-enabled biosensing for SARS-CoV-2 infection have been discussed in detail. This review may serve as a guide for the development of advanced techniques for nanomaterials enabled biosensing to fulfill the present demand of low-cost, rapid and early diagnosis of COVID-19 infection.

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“…Many types of MNPs have been employed to create biosensors for the diagnosis of different respiratory viruses. 97 , 98 These include techniques like magnetic resonance, 99 fluorescence, 100 rolling circle 101 and electrochemical amplification. 102 For example, Zhao et al developed carboxyl polymer‐coated magnetic NPs (pcMNPs) and used this setup to extract viral RNA for accurate SARS‐CoV‐2 diagnosis.…”

Section: Novel Biosensors For the Detection Of Covid‐19mentioning

confidence: 99%

Nanomaterials‐based sensors for the detection of COVID‐19: A review

Naikoo

Arshad

Hassan

et al. 2022

Bioengineering & Transla Med

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With the threat of increasing SARS‐CoV‐2 cases looming in front of us and no effective and safest vaccine available to curb this pandemic disease due to its sprouting variants, many countries have undergone a lockdown 2.0 or planning a lockdown 3.0. This has upstretched an unprecedented demand to develop rapid, sensitive, and highly selective diagnostic devices that can quickly detect coronavirus (COVID‐19). Traditional techniques like polymerase chain reaction have proven to be time‐inefficient, expensive, labor intensive, and impracticable in remote settings. This shifts the attention to alternative biosensing devices that can be successfully used to sense the COVID‐19 infection and curb the spread of coronavirus cases. Among these, nanomaterial‐based biosensors hold immense potential for rapid coronavirus detection because of their noninvasive and susceptible, as well as selective properties that have the potential to give real‐time results at an economical cost. These diagnostic devices can be used for mass COVID‐19 detection to understand the rapid progression of the infection and give better‐suited therapies. This review provides an overview of existing and potential nanomaterial‐based biosensors that can be used for rapid SARS‐CoV‐2 diagnostics. Novel biosensors employing different detection mechanisms are also highlighted in different sections of this review. Practical tools and techniques required to develop such biosensors to make them reliable and portable have also been discussed in the article. Finally, the review is concluded by presenting the current challenges and future perspectives of nanomaterial‐based biosensors in SARS‐CoV‐2 diagnostics.

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Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection

Cited by 10 publications

References 17 publications

Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices

Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices

Prospects of nanomaterials-enabled biosensors for COVID-19 detection

Nanomaterials‐based sensors for the detection of COVID‐19: A review

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