Innate immune training and metabolic reprogramming in primary monocytes of broiler and laying hens

Verwoolde, Michel B.; Biggelaar, Robin H.G.A. van den; Reilingh, G. de Vries; Arts, J.A.J.; Baal, J. van; Lammers, A.; Jansen, Christine A.

doi:10.1016/j.dci.2020.103811

Cited by 15 publications

(6 citation statements)

References 58 publications

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“…These modifications influence the activity of immune-related genes in innate immune cells ( 37 ). For example, in vitro trained chicken macrophages showed enhanced expression of B cell activating molecule CD40 ( 38 ). Thus, trained macrophages may enhance B cell activation in vivo as well, resulting in enhanced antibody responses.…”

Section: Discussionmentioning

confidence: 99%

Transgenerational Effects of Maternal Immune Activation on Specific Antibody Responses in Layer Chickens

et al. 2022

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Activation of the maternal immune system may affect innate and adaptive immune responses in the next generation and may therefore have implications for vaccine efficacy and dietary immune modulation by feed additives. However, transgenerational effects on immune responses in chickens have been investigated to a limited extend. The present study investigated effects of intratracheal (i.t) specific and aspecific immune activation of laying hens on specific antibody production in the next generation. In two experiments laying hens received intratracheally an immune stimulus with human serum albumin (HuSA) or lipopolysaccharide (LPS). In experiment 1, hatchlings of the immune activated hens were at 4 weeks i.t. immunized with HuSA or HuSA+LPS. Maternal immune activation with LPS increased HuSA specific IgY and IgM responses in offspring. These results suggest a transgenerational effect of the maternal immune system on the specific antibody response in the next generation. In experiment 2 hatchlings received either β-glucan-enriched feed or control feed and were i.t. immunized with HuSA. Maternal immune activation with LPS decreased IgY anti-HuSA responses after HuSA immunization within hatchlings that received β-glucan enriched feed. The results of Experiment 2 suggest a transgenerational link between the innate immune system of mother and specific antibody responses in offspring. Despite variabilities in the outcomes of the two experiments, the observations of both suggest a link between the maternal innate immune system and the immune system of the offspring. Furthermore, our results may imply that maternal activation of the innate immune system can influence immune modulating dietary interventions and vaccine strategies in the next generation.

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Section: Discussionmentioning

confidence: 99%

Transgenerational Effects of Maternal Immune Activation on Specific Antibody Responses in Layer Chickens

et al. 2022

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“…Moreover, dietary β-glucan altered the intestinal cytokine–chemokine balance[ 50 ] and intestinal immune-related gene expression profiles, favoring an enhanced Th1 cell response during coccidiosis[ 54 ]. In studies by Verwoolde et al[ 48 , 55 ], chicken monocytes primarily stimulated with β-glucan plus IL-4, followed by secondary stimulation with LPS, demonstrated elevated expression of colony-stimulating factor 1 receptor (CSF-1R), the activation markers CD40 and MHC class-II, and enhanced nitric oxide (NO) production. The results suggested that trained innate immunity may be induced in primary chicken monocytes by β-glucan.…”

Section: Do Vaccination and β-Glucan Supplementation Enhance Innate I...mentioning

confidence: 99%

“…The authors also compared the effects of β-glucan treatment between the monocytes of laying and broiler hens and demonstrated that IL-1β mRNA expression, inducible nitric oxide synthase (iNOS), and hypoxia-inducible factor 1α expression was increased in both breeds. However, the enhancement of CD40 expression and NO production was only observed in layers, suggesting differential effects of β-glucan between the two breeds[ 55 ].…”

Section: Do Vaccination and β-Glucan Supplementation Enhance Innate I...mentioning

confidence: 99%

Innate Immune Training in Chickens for Improved Defense against Pathogens: A Review

Yoshimura,

Nii,

Isobe

2024

J. Poult. Sci.

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The avian immune system plays a vital role in poultry production to obtain good productibility and products that are safe and of high quality. Historically, adaptive immunity has been the main target of vaccination. However, over the past decade, innate immunity has been reported to be enhanced in different animals through vaccination and feed additives. This enhancement is due to innate immune memory termed “trained immunity,” in which epigenetic and metabolic reprogramming play significant roles. Although reports on trained immunity in poultry are limited, several studies have suggested that vaccinations and feed additives affect the innate immunity. This review discusses the possible effects of vaccination and β-glucan on innate immunity for potential incorporation in advanced strategies to enhance the defense function in poultry while considering the information on trained immunity in mammals.

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“…Interestingly, bone marrow–derived adherent cells failed to be trained by this method. In a second paper, Verwoolde et al (68) compared primary blood monocytes from 42-day-old broilers and layers for the ability to induce TII. Trained macrophages responded to stimulation with LPS by significantly increased levels of mRNA for IL-1β, NOS II, and hypoxia-inducible factor 1-α, a master transcription factor controlling inflammatory responses and metabolic reprogramming).…”

Section: Innate Immune Responsesmentioning

confidence: 99%

The Immunological Basis for Vaccination

Rautenschlein,

Schat

2023

Avian Diseases

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Vaccination is crucial for health protection of poultry and therefore important to maintaining high production standards. Proper vaccination requires knowledge of the key players of the well-orchestrated immune system of birds, their interdependence and delicate regulation, and, subsequently, possible modes of stimulation through vaccine antigens and adjuvants. The knowledge about the innate and acquired immune systems of birds has increased significantly during the recent years but open questions remain and have to be elucidated further. Despite similarities between avian and mammalian species in their composition of immune cells and modes of activation, important differences exist, including differences in the innate, but also humoral and cell-mediated immunity with respect to, for example, signaling transduction pathways, antigen presentation, and cell repertoires. For a successful vaccination strategy in birds it always has to be considered that genotype and age of the birds at the time point of immunization as well as their microbiota composition may have an impact and may drive the immune reactions into different directions. Recent achievements in the understanding of the concept of trained immunity will contribute to the advancement of current vaccine types helping to improve protection beyond the specificity of an antigen-driven immune response. The fast developments in new omics technologies will provide insights into protective B-and T-cell epitopes involved in cross-protection, which subsequently will lead to the improvement of vaccine efficacy in poultry.RESUMEN. Estudio recapitulativo-Bases inmunológicas de la vacunación. La vacunación es crucial para la protección de la salud de las aves comerciales y por lo tanto, importante para mantener altos estándares de producción. Una vacunación adecuada requiere el conocimiento de los factores clave del sistema inmunológico bien orquestado de las aves, su interdependencia y su delicada regulación y posteriormente, los posibles modos de estimulación a través de antı genos y adyuvantes de las vacunas. El conocimiento sobre los sistemas inmunológicos innato y adquirido de las aves ha aumentado significativamente durante los últimos años, pero quedan preguntas abiertas que deben dilucidarse con profundidad. A pesar de las similitudes entre las especies de aves y mamı feros en la composición de células inmunes y modos de activación, existen diferencias importantes, incluidas las diferencias en la inmunidad innata, pero también en la inmunidad humoral y en la mediada por células, con respecto a las vı as de transducción de señales, la presentación de antı genos. y repertorios celulares. Para una estrategia de vacunación exitosa en las aves, siempre se debe considerar que el genotipo y la edad de las aves en el momento de la inmunización, ası como la composición de su microbiota pueden tener un impacto y pueden impulsar las reacciones inmunes en diferentes direcciones. Los logros recientes en la comprensión del concepto de inmunidad entrenada contribuirán al avance...

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Innate immune training and metabolic reprogramming in primary monocytes of broiler and laying hens

Cited by 15 publications

References 58 publications

Transgenerational Effects of Maternal Immune Activation on Specific Antibody Responses in Layer Chickens

Transgenerational Effects of Maternal Immune Activation on Specific Antibody Responses in Layer Chickens

Innate Immune Training in Chickens for Improved Defense against Pathogens: A Review

The Immunological Basis for Vaccination

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