Inoculating plant material by jet injection

Wastie, R. L.

doi:10.1111/j.1365-3059.1984.tb00588.x

Cited by 12 publications

(3 citation statements)

References 7 publications

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“…the discoloration of the stem tissue accompanying the bacterial rotting process, invariably originates from a rotting mother tuber (Pérombelon & Kelman, 1980) and therefore, the speed of the rotting process of the tuber tissue, as well as that of the stem tissue, could be regarded as putative components of resistance. Several methods have been described for the screening of tuber tissue resistance (see Allefs et al, 1995a) or stem tissue resistance (Hidalgo & Echandi, 1982;Hossain & Logan, 1983;Wastie, 1984;Lapwood & Read, 1986). In a previous study (Allefs et al, 1995b), it was shown that tuber tissue resistance of stored tubers of a set of 12 cultivars was not very well correlated with resistance to blackleg.…”

Section: Introductionmentioning

confidence: 99%

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

et al. 1996

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The various phases of the infection process in potato blackleg, caused by pectolytic Erwinia spp., were interpreted as components of partial resistance and studied in detail. Mother tubers of glasshouse-grown plants of two potato cultivars were inoculated with antibiotic resistant marker strains of Erwinia carotovora subsp. atroseptica (Eca) or E. chrysanthemi (Ech). Stem tissue samples of these plants were collected 3, 7, 14 and 21 days after inoculation and subsequently analysed for the presence of the bacteria. As soon as 3 days after inoculation, Erwinia bacteria were detected in the stem tissue of the plants. However, blackleg developed only when the rotting mother tuber tissue reached the sites where the stems were attached to the mother tuber. An inoculation method was developed for determining cultivar differences in the incidence and rate at which rot in tuber cylinders proceeds to sprouts on top of such cylinders. A set of 12 cultivars was screened for this putative component of resistance which was called 'stem base resistance'. Significant differences for stem base resistance were found among the cultivars. These differences were clearer when inoculation was carried out with Ech in contrast to Eca. Glasshouse-grown plants of the same set of cultivars were also screened for resistance of the above ground part of the stem. Cultivars differed significantly for this so-called 'stem tissue resistance', but results of some cultivars were not consistent among years of testing. Multiple regression analysis revealed that stem base resistance, stem tissue resistance, tuber tissue resistance and resistance of mother tubers in the field account for 63% (Eca) and 75% (Ech) of the variance found after screening for resistance to blackleg in the field. It was concluded that if results of this study are extrapolated to any set of potato clones, selection for resistance in breeding programmes under laboratory or glasshouse conditions, would be most efficient when directed to clonal differences for stem base resistance.

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Section: Introductionmentioning

confidence: 99%

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

et al. 1996

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“…Potato genotypes have been screened for tuber resistance to erwinias in many countries and differences in resistance have been found (Dobias, 1973;Munzert & Hunnius, 1980;Bourne et al,, 1981;Hidalgo & Echandi, 1982;Ciampi-Panno & Andrade-Soto, 1984;Lapwood et al,, 1984). Different methods have been used to inoculate potato tubers with erwinias (De Boer & Kelman, 1978;Munzert & Hunnius, 1980;Bourne er a/., 1981;Lapwood era/., 1984;Wastie, 1984) but there are relatively few reports comparing results obtained with different methods (Dobias, 1973;Komorowska-Jedrys, 1975;Lapwood et ai, 1984). This paper reports investigations into the effects on cultivar resistance ranking of method of testing, choice of tuber tissue for inoculation, concentration of oxygen during tuber incubation, and method of assessing soft rot development.…”

Section: Introductionmentioning

confidence: 99%

Methods of testing potato cultivars for resistance to soft rot of tubers caused by Erwinia carotovora subsp. atroseptica

Bain¹,

Pérombelon²

1988

Plant Pathology

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The resistance of eight potato cultivars to tuber soft rot caused by E. carotovora subsp. atroseptica was assessed in 2 years using three different test methods. Similar cultivar resistance rankings were obtained for any one method within a year and between years for two methods (single site, infectivity titration), but not for the third (vacuum infiltration). However, the ranking of cultivars differed for the three methods. Ranking was not affected by inoculating the cortex or the more susceptible medullary tissue, or by assessing rotting in terms of infection frequency or lesion size, but it was affected by oxygen concentration during incubation. Differences among cultivars were greater when inoculated tubers were incubated anaerobically than when incubated with 5% oxygen. There was no relationship between the relative susceptibilities of cultivars to tuber soft rot in storage in January/February and those of mother tubers after planting.

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“…Various methods for the inoculation of seed tubers prior to field assessment have been proposed. These include, dipping at harvest (Maas Geesteranus & Vruggink, 1976) or prior to planting (Logan & Copeland, 1979), stabbing with an impregnated tooth pick (Hossain & Logan, 1983) or needle (Logan, 1969;Lapwood & Legg, 1983), or injection by high pressure jet (Wastie, 1984), or under vacuum (Perombelon & Lowe, 1979;McGuire & Kelman, 1984).…”

Section: Introductionmentioning

confidence: 99%

A comparison of methods of seed tuber inoculation for assessing the susceptibility of potato cultivars to blackleg (Erwinia carotovora subsp. atroseptica) in the field

Lapwood

Read

1986

Annals of Applied Biology

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S U M M A R YExperiments were done with the aim of developing a reliable method for assessing the susceptibility of potato cultivars to blackleg caused by Erwinia carotouora subsp. atroseptica, in the field. Over four years seed tubers were either stab-inoculated at the heel-end prior to planting, vacuum infiltrated with bacteria just after harvest or vacuum infiltrated prior to sprouting and after 'cutting' or 'pricking'.Stab inoculation produced fewer diseased plants but generally a greater range of symptoms than vacuum infiltration. Vacuum infiltrated 'cut' seed gave most disease whereas infiltration at harvest and infiltration of 'pricked' seed gave similar disease incidence. The cultivar Maris Piper was more resistant than DCsirCe in 1981 and 1982, and Pentland Javelin than Ulster Sceptre in 1983 and 1984, largely irrespective of the inoculation treatment. Irrigation improved yields but did not affect disease symptoms.In further field experiments over three years, each with a minimum of 20 cultivars, seed tubers were sliced just before planting at a standard distance from the apex and inoculated by applying a pad impregnated with inoculum. Differences were shown between cultivars and it is suggested that the method could be adapted for testing for blackleg susceptibility under controlled environment conditions.

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Inoculating plant material by jet injection

Cited by 12 publications

References 7 publications

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

Methods of testing potato cultivars for resistance to soft rot of tubers caused by Erwinia carotovora subsp. atroseptica

A comparison of methods of seed tuber inoculation for assessing the susceptibility of potato cultivars to blackleg (Erwinia carotovora subsp. atroseptica) in the field

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