Inositol Hexaphosphate (IP6) Inhibits Cellular Proliferation In Melanoma

Rizvi, Irfan; Riggs, Dale R.; Jackson, Barbara; Ng, Alex H. M.; Cunningham, Cynthia; McFadden, David W.

doi:10.1016/j.jss.2006.02.023

Cited by 33 publications

(21 citation statements)

References 11 publications

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“…Herein, we reported profound reductions in cellular proliferation ranging from 37.1 to 91.5%. We observed similar results when IP6 was administered to melanoma cells in vitro, with mean inhibitions of 52% after only 72 h of incubation (15). In our current study, significant growth inhibition was induced by the addition of IP6 in vitro to both Barrett's adenocarcinoma cell lines.…”

Section: Discussionsupporting

confidence: 82%

“…We previously evaluated the effects of in vitro IP6 administration on VEGF production in the HTB68 melanoma cell line (15). VEGF production was significantly reduced using IP6 by an astounding 81.7%.…”

Section: Discussionmentioning

confidence: 99%

“…IP6 is also present in almost all mammalian cells, where it regulates cell function, proliferation and differentiation (5). IP6, a natural antioxidant (6), has been shown to express significant inhibitory effects against a variety of primary tumors such as breast (7,8), colon (9), liver (10), prostate (11-13) pancreas (14) and melanoma (15). The effects of IP6 have not been evaluated in Barrett's adenocarcinoma; therefore we hypothesized that IP6 would significantly inhibit the cell growth rate of Barrett's adenocarcinoma in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Corn-derived carbohydrate inositol hexaphosphate inhibits Barrett's adenocarcinoma growth by pro-apoptotic mechanisms

McFadden

Riggs²,

Jackson³

et al. 2008

Oncol Rep

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Abstract. Inositol hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate that is found in food sources high in fiber content. IP6 has been reported to have significant inhibitory effects against a variety of primary tumors. We hypothesized that IP6 would inhibit the cell growth rate of Barrett's adenocarcinoma in vitro. Two Barrett'sassociated adenocarcinoma cell lines, SEG-1 and BIC-1, were treated with IP6 at 0.5, 1.0 and 5.0 mM concentrations. Cell viability was measured by MTT assay. Apoptosis and necrosis were evaluated by the Annexin V FITC assay. Reductions (P<0.001) in cellular proliferation were observed in both cell lines. IP6 decreased late apoptosis and necrosis in BIC cells, whereas in SEG-1 cells, early apoptosis, late apoptosis and necrosis were all increased by IP6. IP6 decreases cellular growth by pro-apoptotic mechanisms. Our findings suggest that IP6 has the potential to become an effective adjunct for Barrett's adenocarcinoma. Further studies are needed to evaluate safety and clinical utility of this agent in patients with Barrett's adenocarcinoma.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Corn-derived carbohydrate inositol hexaphosphate inhibits Barrett's adenocarcinoma growth by pro-apoptotic mechanisms

McFadden

Riggs²,

Jackson³

et al. 2008

Oncol Rep

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“…Melanoma is the most lethal form of skin cancer and accounts for 75% of skin cancer-related deaths (Rizvi et al, 2006;Schneider et al, 2009;Osmond et al, 2012). At present, due to western medical treatments for cancer have side-effects, some attentions have shifted to discover new anti-cancer strategy, such as the use of traditional Chinese medicine, in the hope of producing anti-tumor effect without too many serious side-effects (Treasure et al, 2005;Cho et al, 2007;Li et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Although those treatments are effective in many patients, not all patients will respond and many will have recurrence and/or progression of the melanoma. For these patients, new and potentially more effective forms of therapy need to be developed to improve survival rates (Rizvi et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antitumor Efficacy with Combined Administration of Astragalus and Pterostilbene for Melanoma

Huang¹,

Zhang²,

Wang³

2014

Asian Pacific Journal of Cancer Prevention

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Astragalus, a commonly used traditional Chinese medicine, has exhibited antitumor actions in patients. In this study, in vitro and in vivo antitumor effects of astragalus and synergistic antitumor efficacy in combination with pterostilbene were investigated. Melanoma cells were treated with pterostilbene (Pt), graduated doses of astragalus injection (AI), or these in combination. Cell viability was measured using a MTT assay. Released nucleosomes and caspase activity were measured using enzyme-linked immunosorbent assay. Growth inhibition in vitro and in vivo was also assessed. Analysis of variance and t tests were used for statistical analysis. Significant reduction (p<0.05) in cellular proliferation were observed with AI and AI-Pt in a time-and concentration-dependent manner. Apoptosis and caspase-3/7 activity were significantly increased by AI and AI-Pt treatment (p<0.05). In vivo, AI inhibited melanoma tumor growth, with inhibition rates ranging from 36.5 to 62.3%, by inducing apoptosis via up-regulation Bax expression and the Bax/Bcl-2 ratio and down-regulating Bcl-2 expression. AI significantly inhibits the growth of melanoma in vitro and in vivo by inducing apoptosis. These data suggest that combined treatment of astragalus with pterostilbene enhances antitumor efficacy.

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Simultaneous determination of inositol and inositol phosphates in complex biological matrices: quantitative ion‐exchange chromatography/tandem mass spectrometry

Liu

Villalta

Sturla

2009

Rapid Comm Mass Spectrometry

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myo-Inositol (Ins) and myo-inositol phosphates (InsPs) are widely distributed in plants and animals. The evaluation of the distribution of Ins and InsPs in cells and plant sources can impact the understanding of their role in nutrition, cellular processes and diseases, and how they may be modulated by diet. We developed an anion-exchange chromatography/tandem mass spectrometry (HPLC/ESI-MS/MS) method for the separation and simultaneous quantitation of Ins and different naturally occurring phosphorylated inositol compounds. Chromatographic separation was achieved in 30 min on a commercial anion-exchange column (0.5 x 150 mm) using a gradient of 200 mM ammonium carbonate buffer (pH 9.0) and 5% methanol in H(2)O. Analytes were identified by selective reaction monitoring using a triple quadrupole mass spectrometer in negative ion electrospray ionization mode. Adenosine 5'-monophosphate was used as a general internal standard for quantitation. Detection is linear in the range of 0.25-400 pmol for Ins, InsP(1), InsP(4), and InsP(5), 40-400 pmol for InsP(2) and InsP(3), and 60-400 pmol for InsP(6), with a minimum r(2) > 0.994. The limit of detection is 0.25 pmol with a signal-to-noise ratio of 10:1 for all analytes. The intra-day and inter-day variations were within 17% at three concentration levels. Recovery values for the seven analytes spiked into extraction solution or different matrices were between 63 and 121%. Using this approach, Ins and InsPs were measured in three different plant samples and in cultured cells, illustrating significant differences in the distribution of inositol compounds in food samples compared to cells and between cell types.

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Inositol Hexaphosphate (IP6) Inhibits Cellular Proliferation In Melanoma

Cited by 33 publications

References 11 publications

Corn-derived carbohydrate inositol hexaphosphate inhibits Barrett's adenocarcinoma growth by pro-apoptotic mechanisms

Corn-derived carbohydrate inositol hexaphosphate inhibits Barrett's adenocarcinoma growth by pro-apoptotic mechanisms

Enhanced Antitumor Efficacy with Combined Administration of Astragalus and Pterostilbene for Melanoma

Simultaneous determination of inositol and inositol phosphates in complex biological matrices: quantitative ion‐exchange chromatography/tandem mass spectrometry

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