Insect Pathogenic Bacteria in Integrated Pest Management

Ruiu, Luca

doi:10.3390/insects6020352

Cited by 165 publications

(114 citation statements)

References 79 publications

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“…S4 in the supplemental material). Interestingly, insect-pathogenic bacteria, including Photorhabdus and Xenorhabdus species, are commonly studied and developed as biological agents for pest control (45). It prompts us to consider the possibility that the spread of Photorhabdus and Xenorhabdus in the environment may have facilitated the transfer, directly or via an intermediate, of PirA/PirB toxins into V. parahaemolyticus, which leads to the emergence of AHPND.…”

Section: Discussionmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires

Kinch

Ray

Dalia

et al. 2017

Appl Environ Microbiol

105

116

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Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. AHPND is caused by toxic strains of Vibrio parahaemolyticus that have acquired a "selfish plasmid" encoding the deadly binary toxins PirA vp /PirB vp . To better understand the repertoire of virulence factors in AHPND-causing V. parahaemolyticus, we conducted a comparative analysis using the genome sequences of the clinical strain RIMD2210633 and of environmental non-AHPND and toxic AHPND isolates of V. parahaemolyticus. Interestingly, we found that all of the AHPND strains, but none of the non-AHPND strains, harbor the antibacterial type VI secretion system 1 (T6SS1), which we previously identified and characterized in the clinical isolate RIMD2210633. This finding suggests that the acquisition of this T6SS might confer to AHPND-causing V. parahaemolyticus a fitness advantage over competing bacteria and facilitate shrimp infection. Additionally, we found highly dynamic effector loci in the T6SS1 of AHPND-causing strains, leading to diverse effector repertoires. Our discovery provides novel insights into AHPND-causing pathogens and reveals a potential target for disease control.IMPORTANCE Acute hepatopancreatic necrosis disease (AHPND) is a serious disease that has caused severe damage and significant financial losses to the global shrimp industry. To better understand and prevent this shrimp disease, it is essential to thoroughly characterize its causative agent, Vibrio parahaemolyticus. Although the plasmidencoded binary toxins PirA vp /PirB vp have been shown to be the primary cause of AHPND, it remains unknown whether other virulent factors are commonly present in V. parahaemolyticus and might play important roles during shrimp infection. Here, we analyzed the genome sequences of clinical, non-AHPND, and AHPND strains to characterize their repertoires of key virulence determinants. Our studies reveal that an antibacterial type VI secretion system is associated with the AHPND strains and differentiates them from non-AHPND strains, similar to what was seen with the PirA/ PirB toxins. We propose that T6SS1 provides a selective advantage during shrimp infections.

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Section: Discussionmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires

Kinch

Ray

Dalia

et al. 2017

Appl Environ Microbiol

105

116

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“…PGPB are able to control pathogens by several mechanisms: competitive root colonization, production of antimicrobial compounds [11], production of hydrolytic enzymes, siderophores, HCN, ammonia, and by inducing systemic plant resistance [49]. In addition, insecticidal activities were reported for some PGPB in addition to the existing biocontrol repertoire with perspectives for application against insect crop pests [48]. Recent reports suggest that PGPB also enhance the tolerance of plants towards abiotic stresses such as drought [54,58], chilling injury [3], salinity [22], metal toxicity [13] and elevated temperature stress [1,2].…”

Section: Introductionmentioning

confidence: 99%

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Bensidhoum

Nabti

Tabli

et al. 2016

European Journal of Soil Biology

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The application of plant growth-promoting bacteria (PGPB) with biocontrol activities as inoculants of crops plants against phytopathogenic fungi and insect pests provides a biological alternative to the use of agrochemicals. Two Pseudomonas sp. strains were isolated from agricultural well water in the area of Bejaia, northeastern Algeria, located rather closely to a lead mine deposit. The isolates S4LiBe and S5LiBe had 16S rRNA gene sequence similarities of 99.4% to 99.7% with Pseudomonas protegens CHAO T and other P. protegens strains. The phenotypic profiles tested with BIOLOG-GN2-microplates were very similar, but showed also some remarkable differences. The isolates S4LiBe and S5LiBe showed plant growthpromoting potential based on the production of the phytohormone indole acetic acid and siderophores and the solubilization of insoluble phosphate. In addition, they produced chitinase and other polymer degrading enzymes. Interestingly, while S4LiBe and S5LiBe were resistant against heavy metals (2.0 mM K2Cr2O7 and 3.0 mM CoSO4, HgSO4, CdSO4 8H2O and PbCl2), the reference strain P. protegens CHAO T was very sensitive to Hg 2+ and Cd 2+ and had lower tolerance towards Co 2+ and Pb 2+ . The isolates S4LiBe and S5LiBe were very active in mycelial growth inhibition assays against Botrytis cinerea, Verticillium dahlia, Fusarium graminearum, Aspergillus niger and A. flavus (growth inhibition between 88% and 48%). Furthermore, S4LiBe and S5LiBe showed effective insecticidal activities, when tested in the Galleria injection assay and they were tested positive for the insect toxin gene fitD alike the reference strain CHA0 T . Finally, inoculation of barley seeds with S5LiBe resulted in significantly stimulated germination rate and growth of seedlings, with increased shoot length, shoot and root fresh weight, shoot and root dry weight as compared to non-inoculated plants.Thus, the heavy metal tolerant isolates S4LiBe and S5LiBe harbor a diverse potential as beneficial bacteria for agricultural application. They may be very useful even in polluted soils for the stimulation of e.g. biomass crops. The demonstration of successful isolation from agricultural well water may open more ready access for a wide variety of this kind of beneficial bacteria for agricultural application.

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“…In case of Fusarium, it commonly attacks on xylem and phloem of plants (Kiffer & Morelet, 1997). Streptomyces is classified as actionbacteria that have gained high commercial interest for the production of a variety of metabolites acting as potential insecticides (Ruiu, 2015). In addition, Penicilium and Gliocladium are potential antagonistic fungi (Gouli, V., Gouli, S., Marcelino, Skinner, & Parker, 2013;Vázquez-Martínez, Cirerol-Cruz, Torres-Estrada, & López, 2014).…”

Section: Macroscopic and Microscopic Characteristic Of Microbial Isolmentioning

confidence: 99%

MICROBIAL COMMUNITY ASSOCIATED WITH AMBROSIA BEETLE, Euplatypus parallelus ON SONOKEMBANG, Pterocarpus indicus IN MALANG

Tarno

Septia²,

Aini³

2016

Agrivita.J.Agr.Sci

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Recently, most of sonokembang, Pterocarpus indicus trees are dying in Malang. In 2012, the death rate of trees reached ca. 11%. In addition, death of trees spread to other regencies in East Java. Euplatypus parallelus is a specific species of ambrosia beetles that were the causal agents to the dying and wilting of sonokembang trees in Malang. Wilting is caused mainly by the pathogenic fungi carried by ambrosia beetles. To confirm the microbial communities related to E. parallelus that attack sonokembang, E. parallelus and some attacked trees were collected in Malang city. Isolation and identification of these species were conducted at the Laboratory of Mycology, Faculty of Agriculture, University of Brawijaya and Laboratory of Molecular Biology, Islamic State University, Malang. Results showed that there were nine microbes including five genera of fungi, two genera of yeasts and one genus of bacterium were identified. The microbial communities that were found namely Aspergillus spp., Penicillium spp., Trichoderma spp., Fusarium spp., Acremonium spp., Gliocladium spp. (fungi), Streptomyces spp. (bacteria), Saccharomyces spp., and Candida spp. (yeast).

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Insect Pathogenic Bacteria in Integrated Pest Management

Cited by 165 publications

References 79 publications

Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires

Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

MICROBIAL COMMUNITY ASSOCIATED WITH AMBROSIA BEETLE, Euplatypus parallelus ON SONOKEMBANG, Pterocarpus indicus IN MALANG

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