Insect-toxic secreted proteins and virulence of the entomopathogenic fungus Beauveria bassiana

Ortiz‐Urquiza, Almudena; Riveiro-Miranda, L.; Santiago‐Álvarez, C.; Quesada‐Moraga, Enrique

doi:10.1016/j.jip.2010.07.003

Cited by 59 publications

(24 citation statements)

References 43 publications

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“…Among all the tested species, B. bassiana was the one that showed the greatest virulence. B. bassiana killed more individuals in shorter time, probably due to a higher germination speed of conidia and penetration in the cuticle and tissue infection, as well as by a greater production of virulence factors (Ortiz‐Urquiza, Luo, & Keyhani, ; Ortiz‐Urquiza, Riveiro‐Miranda, Santiago‐Álvarez, & Quesada‐ Moraga, ; Quesada‐Moraga & Vey, ; Zimmermann, ). These results agree with those from Soliman () who evaluated the in vitro virulence of formulated products of B. bassiana , Metarhizium anisopliae Sorokin, Fusarium proliferatum and Lecanicillium longisporum (Petch) Zare & W. Gams , finding the highest T. peregrinus mortality in the shortest time with the B. bassiana formulation.…”

Section: Discussionmentioning

confidence: 99%

Entomopathogenic fungi naturally infecting the eucalypt bronze bug,Thaumastocoris peregrinus(Heteroptera: Thaumastocoridae), in Uruguay

Corallo

Simeto

Martínez

et al. 2019

J Applied Entomology

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Thaumastocoris peregrinus Carpintero & Dellapé (Heteroptera: Thaumastocoridae) is a sap‐sucking insect that feeds on leaves of Eucalyptus. In Uruguay, it was detected in 2008 causing significant economic losses in Eucalyptus plantations. At present, there is no efficient control for this pest; thus, the use of biological control agents seems to be an environmentally friendly alternative to reduce the damage caused by this insect. The aims of this study were to isolate and identify the species of entomopathogenic fungi that naturally infect T. peregrinus in Uruguay and to characterize and select the most virulent isolates towards this pest. Individuals of T. peregrinus were collected in eight Eucalyptus plantations infested by the pest. The entomopathogenic fungi were isolated and identified by observation of their micromorphological characteristics, and their identity was confirmed by molecular methods. The pathogenicity and virulence against T. peregrinus of the isolated entomopathogenic fungi were evaluated. Isolates causing the highest insect mortality were selected to evaluate the effect of temperature and water activity on conidial viability. Entomopathogenic fungi were found in very low number with the prevalence of less than 3%. Isolates corresponded to Beauveria, Fusarium, Isaria, Lecanicillium, Paecilomyces, Pochonia, Purpureocillium and Simplicillium genera. Two species, B. pseudobassiana and L. muscarium, were first recorded in Uruguay. Among all the tested species, an isolate of B. bassiana (FI 2403) showed the highest virulence followed by an isolate of B. pseudobassiana. The isolate of B. bassiana presented the highest percentage of spore germination at the three temperatures and the highest viability at low water activities. Isolate FI 2403 was selected as a promissory candidate for the development of a commercial formulation against T. peregrinus.

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Section: Discussionmentioning

confidence: 99%

Entomopathogenic fungi naturally infecting the eucalypt bronze bug,Thaumastocoris peregrinus(Heteroptera: Thaumastocoridae), in Uruguay

Corallo

Simeto

Martínez

et al. 2019

J Applied Entomology

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“…The low virulence of P. lilacinum compared with other entomopathogenic fungi species could be related to the low production of toxins, proteases, and chitinases associated with the infection process of this fungus (Khan et al 2004). Virulence is a characteristic regulated by the expression of genes that encode extracellular proteins or toxins (Quesada-Moraga & Vey 2003;Gao et al 2011;Ortiz-Urquiza et al 2010;Pedrini et al 2013;Staats et al 2014). The expression of genes can be modified by the density and viability of the inoculum (Herlinda 2010), the number of subcultures, and the nutritive quality from culture medium where the inoculum is multiplied, and finally the inoculation methods and environmental conditions in which the experiment is conducted (Quesada-Moraga & Vey 2003;Ortiz-Urquiza et al 2010;Inglis et al 2012).…”

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confidence: 99%

Pathogenicity and Virulence of Purpureocillium lilacinum (Hypocreales: Ophiocordycipitaceae) on Mexican Fruit Fly Adults

Toledo-Hernández

Toledo

Valle

et al. 2019

Florida Entomologist

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“…In addition, Chong-Rodríguez et al ([2011]) described that BS of B. bassiana maintained for six months at 4°C showed a mortality of more than 80% against third-instar P. xylostella larvae 8 days after application. Furthermore, Ortiz-Urquiza et al [2010] observed that the composition of the culture medium affected the virulence of AC from B. bassiana because of an increased or decreased secretion of virulent proteins. But the influence of the culture media on the virulence of B. bassiana was not investigated in this work.…”

Section: Discussionmentioning

confidence: 99%

Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

2014

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A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic Beauveria bassiana isolate ATP-02. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. B. bassiana was raised in shake flask cultures to produce high concentrations of total spores (TS), which include blastospores (BS) and submerged conidiospores (SCS). The highest concentration of 1.33×109 TS/mL and the highest yield of 5.32×1010 TS/g sucrose was obtained in the TKI broth with 5% sugar beet molasses which consists of 50% sucrose as a carbon source. In spite of the lower sugar concentration (2.5%) the amount of TS could be increased up to 11-times in contrast to the cultivation with 5% sucrose. The scale-up to a 2 L stirred tank reactor was carried out at 25°C, 200–600 rpm and 1 vvm at pH 5.5. A TS yield of 5.2×1010 TS/g sucrose corresponding to a SCS yield of 0.2×1010 SCS/g sucrose was obtained after 216 h. With regards to the culture medium the cost of 1012 TS amounts to 0.24 €. Plutella xylostella larvae, which were fed with oilseed rape leaves treated with spores from fermentation resulted in 77 ± 5% mortality. Moreover, spores from submerged cultivation were able to colonize oilseed rape leaves via leaf application. This is the first report of fermentation of an endophytic B. bassiana strain in a low-cost culture medium to very high yields of TS.

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Insect-toxic secreted proteins and virulence of the entomopathogenic fungus Beauveria bassiana

Cited by 59 publications

References 43 publications

Entomopathogenic fungi naturally infecting the eucalypt bronze bug,Thaumastocoris peregrinus(Heteroptera: Thaumastocoridae), in Uruguay

Entomopathogenic fungi naturally infecting the eucalypt bronze bug,Thaumastocoris peregrinus(Heteroptera: Thaumastocoridae), in Uruguay

Pathogenicity and Virulence of Purpureocillium lilacinum (Hypocreales: Ophiocordycipitaceae) on Mexican Fruit Fly Adults

Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

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