Insights into the binding of the drugs diclofenac sodium and cefotaxime sodium to serum albumin: Calorimetry and spectroscopy

Sharma, Ruchika; Choudhary, Sinjan; Kishore, Nand

doi:10.1016/j.ejps.2012.03.007

Cited by 50 publications

(31 citation statements)

References 36 publications

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“…This indicates that, binding of HA to domain III is stronger as compare to domain II whereas, negligible to domain I as higher energy is required to change from the liganded native state into the free unfolded state in case of domain III followed by domain II. Thus, HA preferential binds to the folded or native form of the HSA which causes stabilization of the folded state and hence unfolding of HSA become progressively less favorable as HA concentration increases [41], [42].…”

Section: Resultsmentioning

confidence: 99%

A Comprehensive Insight into Binding of Hippuric Acid to Human Serum Albumin: A Study to Uncover Its Impaired Elimination through Hemodialysis

et al. 2013

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Binding of hippuric acid (HA), a uremic toxin, with human serum albumin (HSA) has been examined by isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), molecular docking, circular dichroism (CD) and fluorescence spectroscopy to understand the reason that govern its impaired elimination through hemodialysis. ITC results shows that the HA binds with HSA at high (K b ∼104) and low affinity (K b ∼103) sites whereas spectroscopic results predict binding at a single site (K b∼103). The HA form complex with HSA that involves electrostatic, hydrogen and hydrophobic binding forces as illustrated by calculated thermodynamic parameters. Molecular docking and displacement studies collectively revealed that HA bound to both site I and site II; however, relatively strongly to the later. Esterase-like activity of HSA confirms the involvement of Arg410 and Tyr411 of Sudlow site II in binding of HA. CD results show slight conformational changes occurs in the protein upon ligation that may be responsible for the discrepancy in van’t Hoff and calorimetric enthalpy change. Furthermore, an increase in and is observed from DSC results that indicate increase in stability of HSA upon binding to HA. The combined results provide that HA binds to HSA and thus its elimination is hindered.

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Section: Resultsmentioning

confidence: 99%

A Comprehensive Insight into Binding of Hippuric Acid to Human Serum Albumin: A Study to Uncover Its Impaired Elimination through Hemodialysis

et al. 2013

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“…Cefotaxime sodium (CS), a broad spectrum antibiotic, is widely used in treatment of susceptible infections in respiratory tract, skin and skin structure without serious side effects, and it has good activity against Gram-positive and Gram-negative bacteria [25,26]. Dopamine, a mussel-inspired molecule, can undergo self-polymerization and adhere onto almost any solid surface without surface pre-treatments.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Zhou

Wang

et al. 2014

J. R. Soc. Interface.

121

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Implant-associated infections, which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, practical approaches to prevent implant-associated infections are in great demand. Inspired by adhesive proteins in mussels, here we have developed a novel antibiotic-decorated titanium (Ti) material with enhanced antibacterial activity. In this study, Ti substrate was coated by one-step pH-induced polymerization of dopamine followed by immobilization of the antibiotic cefotaxime sodium (CS) onto the polydopamine-coated Ti through catechol chemistry. Contact angle measurement and X-ray photoelectron spectroscopy confirmed the presence of CS grafted on the Ti surface. Our results demonstrated that the antibiotic-grafted Ti substrate showed good biocompatibility and well-behaved haemocompatibility. In addition, the antibiotic-grafted Ti could effectively prevent adhesion and proliferation of Escherichia coli (Gram-negative) and Streptococcus mutans (Gram-positive). Moreover, the inhibition of biofilm formation on the antibiotic-decorated Ti indicated that the grafted CS could maintain its long-term antibacterial activity. This modified Ti substrate with enhanced antibacterial activity holds great potential as implant material for applications in dental and bone graft substitutes.

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“…The increase in PCs rejection values using wastewater Type II could be due to their adsorption onto the proteins, which may form aggregates with higher size than the dissolved protein in the solution [60]. Other researchers as Sharma et al studied the BSA interaction with two different PCs (Diclofenac sodium and Cefotaxime sodium) and they observed that the binding affinity of both PCs with BSA was high in a range of temperatures between 10 and 35 • C. They also demonstrated that a tighter binding BSA and Diclofenac occurred [61]. In addition, Karpii et al corroborated that the presence of albumin in serum diminished the adsorption of PCs onto a PVDF modified membrane [46].…”

Section: Validation Of the Analytical Methodsmentioning

confidence: 92%

Comparison of different removal techniques for selected pharmaceuticals

Vona

Martino

García-Ivars

et al. 2015

Journal of Water Process Engineering

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a b s t r a c tRecently, there is an emergence of endocrine-disrupting compounds, pharmaceuticals, and personal care products (EDC/PPCPs) as important pollutants to remove from drinking water and reclaimed wastewater. In this work, the efficiency of removing pharmaceuticals (PCs) from model aqueous solutions and raw wastewater with ultrafiltration (UF), nanofiltration (NF), activated carbon adsorption (AC), biological methods (SBR) and oxidation with ClO 2 was investigated. Some treatments have also been used as combined processes: UF + NF, UF + AC, SBR + ClO 2 . Ibuprofen, Acetaminophen, Diclofenac, Sulfamethoxazole, Clonazepam, and Diazepam were selected as model compounds. In order to evaluate their removal, PC solutions were also considered at several operating conditions (pH, conductivity, concentration, and temperature), and optimal conditions were obtained. Experiments were performed at usual PC concentrations in wastewaters: 1000 ng/L for Ibuprofen and Acetaminophen, 300 ng/L for Diclofenac, Sulfamethoxazole, Clonazepam, and Diazepam. Separation was evaluated by liquid chromatography-mass spectroscopy. Results indicated that the removal efficiency depends on their Log K OW , which is intrinsically related to their hydrophobicity and then, to their adsorption onto the surface (UF, NF, and AC). Also, NF, AC, and combined processes (UF + NF, UF + AC) were the most suitable separation techniques to obtain high removal efficiencies for most of the PCs used, except for Acetaminophen (which showed great removal efficacy using SBR). UF presented low removal yields for all PCs tested. ClO 2 treatment was more effective at high concentration (50 mg ClO 2 /L). Furthermore, results also showed that there are significant differences on the performance of the processes applied and which treatment is the most effective for each PC analyzed.

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Insights into the binding of the drugs diclofenac sodium and cefotaxime sodium to serum albumin: Calorimetry and spectroscopy

Cited by 50 publications

References 36 publications

A Comprehensive Insight into Binding of Hippuric Acid to Human Serum Albumin: A Study to Uncover Its Impaired Elimination through Hemodialysis

A Comprehensive Insight into Binding of Hippuric Acid to Human Serum Albumin: A Study to Uncover Its Impaired Elimination through Hemodialysis

Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Comparison of different removal techniques for selected pharmaceuticals

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