Insights into the impact of deep eutectic solvents on horseradish peroxidase: Activity, stability and structure

Wu, Ben-Pei; Wen, Qing; Xu, Hòng; Yang, Zhen

doi:10.1016/j.molcatb.2014.01.001

Cited by 143 publications

(121 citation statements)

References 28 publications

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“…And further study on circular dichroism spectroscopy proved that DESs examined were able to increase α-helix content for the enzyme so as to improve its stability (Wu et al 2014).…”

Section: The Effects Of Des On the Activity Of Enzymementioning

confidence: 97%

The effect of deep eutectic solvents on the asymmetric hydrolysis of styrene oxide by mung bean epoxide hydrolases

Peng

Zhao

et al. 2018

Bioresour. Bioprocess.

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Background: Deep eutectic solvents have attracted considerable attention in numerous fields. There is little information on mung bean epoxide hydrolase-catalyzed epoxides in deep eutectic solvent-containing system. Results:Adding deep eutectic solvents with hydrogen bond donor of acids to phosphate buffer resulted in an obvious decrease in the optical purity of product; nevertheless, the relative slight change was observed by the addition of the deep eutectic solvents with hydrogen bond donor of alcohols and urea. Of the tested deep eutectic solvents, 10% additional amount of choline chloride/trithylene glycol can cause a significant improvement in the enantiopurity of product, from 83.2 ± 1.3% to 87.9 ± 0.3%. Moreover, with the increase in the addition amount of choline chloride/ triethylene glycol from 10 to 30%, the enantiomeric excess of product enhanced from 87.9 to 94%, but a decline of product yield was observed. Finally, the evaluation of enzyme stability showed that the additional amount from 10% to 30% was not beneficial to the activity recovery. Conclusion:In short, adding choline chloride/triethylene glycol contributes to the improvement in the optical purity of (R)-1-phenyl-1, 2-ethanediol in the catalysis of styrene oxide by mung bean epoxide hydrolases; meanwhile, the enzyme immobilization could be essential.

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“…And further study on circular dichroism spectroscopy proved that DESs examined were able to increase α-helix content for the enzyme so as to improve its stability (Wu et al 2014).…”

Section: The Effects Of Des On the Activity Of Enzymementioning

confidence: 97%

The effect of deep eutectic solvents on the asymmetric hydrolysis of styrene oxide by mung bean epoxide hydrolases

Peng

Zhao

et al. 2018

Bioresour. Bioprocess.

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“…Garlic bulbs (A. sativum) were purchased from a local supermarket in Shenzhen, China. DESs were prepared by following the procedures described in Wu et al (2014); Huang et al (2014). The DES concentration was defined based on the molar concentration of the cholinium salt.…”

Section: Reagents and Test Organismsmentioning

confidence: 99%

Assessing the toxicity and biodegradability of deep eutectic solvents

et al. 2015

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“…However, DESs formed by EG and Gly as HBDs (ChCl : EG-DES aq , ChCl : Gly-DES aq , EAC : EG-DES aq and EAC : Gly-DES aq ) were relatively harmless, obtaining an LC 50 range from 5400 to 12 500 mg L À1 . 27,68 It was also reported that the hydrogenbond network in DESs lowers the chemical potential of the components of DESs and thereby affected the toxicological prole. The effective concentration was higher (less toxic) for ChCl-based DESs than EAC-based DESs.…”

Section: And the Exact Values Inmentioning

confidence: 99%

Evaluation of toxicity and biodegradability for cholinium-based deep eutectic solvents

2015

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This study presented the toxicological and biodegradable assessment of different cholinium-based deep eutectic solvents (DESs). They were formed from choline chloride (ChCl) and N,N-diethyl ethanol ammonium chloride (EAC) as salts and four hydrogen bond donors, namely ethylene glycol (EG), glycerol (Gly), urea (U), malonic acid (MA), in addition to a metal salt, i.e. zinc chloride (ZnCl 2 ), and a hydrated metal salt, i.e. zinc nitrate hexahydrate (ZnN). The toxicity towards Aspergillus niger of pure and aqueous DESs was evaluated by observing the inhibition zone using an agar well diffusion assay, and the minimum inhibition concentration (MIC) using a broth dilution assay, respectively. The MIC values of the DESs varied from 1 to 650 mg mL À1 , whereas the inhibition zones changed according to the DES dose amount. Another test for acute toxicity was performed by evaluating the lethal concentration at 50% (LC 50 ) of the same DESs on Cyprinus carpio fish. The LC 50 of DESs ranged from practically harmless (e.g.ChCl : EG-DES aq ) to highly toxic (e.g. EAC : ZnCl 2 -DES aq ). The toxicity profile of the DESs depended on their concentration, type of individual components, and interaction with living organisms. Moreover, the DESs recorded higher toxicity compared to their individual components on fungi. However, lower toxicity was found for the DESs tested on Cyprinus carpio. Types I (organic salts and metal salt) and II (organic salt and hydrate metal salt) eutectics exhibited significantly higher toxicity than type III (organic salts and HBD). This was due to the presence of the innate toxicity of the metal salts. The biodegradability was appraised by a closed bottle test in which all the DESs were found to be readily biodegradable. To the best of our knowledge, there are no previous studies reported regarding the toxicity of cholinium-based DESs on freshwater fish or fungi and the biodegradability of EAC-based DESs. Therefore, this investigation can be used as a benchmark for future development of DESs. Table 1 Structures, names, and abbreviations of the individual components of the tested DESs Ammonium salts Hydrogen bond donors/metal salt/hydrate metal salt This journal is

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Insights into the impact of deep eutectic solvents on horseradish peroxidase: Activity, stability and structure

Cited by 143 publications

References 28 publications

The effect of deep eutectic solvents on the asymmetric hydrolysis of styrene oxide by mung bean epoxide hydrolases

The effect of deep eutectic solvents on the asymmetric hydrolysis of styrene oxide by mung bean epoxide hydrolases

Assessing the toxicity and biodegradability of deep eutectic solvents

Evaluation of toxicity and biodegradability for cholinium-based deep eutectic solvents

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