Insights into the improvement of the enzymatic hydrolysis of bovine bone protein using lipase pretreatment

Yao, Yumei; Wang, Mengyan; Liu, Ye; Han, Lujia; Liu, Xian

doi:10.1016/j.foodchem.2019.125199

Cited by 32 publications

(13 citation statements)

References 29 publications

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“…Enzyme is an effective biocatalyst which can biodegrade substances specifically under mild conditions (Brandelli et al, 2015;Yao et al, 2020). Enzymes have specific active sites, ones which are able to bind with specific substrates and reduce the activation energy by this approach during enzymatic processes.…”

Section: Introductionmentioning

confidence: 99%

Roles and applications of enzymes for resistant pollutants removal in wastewater treatment

Feng

Ngo

Guo

et al. 2021

Bioresource Technology

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Resistant pollutants like oil, grease, pharmaceuticals, pesticides, and plastics in wastewater are difficult to be degraded by traditional activated sludge methods. These pollutants are prevalent, posing a great threat to aquatic environments and organisms since they are toxic, resistant to natural biodegradation, and create other serious problems. As a high-efficiency biocatalyst, enzymes are proposed for the treatment of these resistant pollutants. This review focused on the roles and applications of enzymes in wastewater treatment. It discusses the influence of enzyme types and their sources, enzymatic processes in resistant pollutants remediation, identification and ecotoxicity assay of enzymatic transformation products, and typically employed enzymatic wastewater treatment systems.Perspectives on the major challenges and feasible future research directions of enzyme-based wastewater treatment are also proposed.

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Section: Introductionmentioning

confidence: 99%

Roles and applications of enzymes for resistant pollutants removal in wastewater treatment

Feng

Ngo

Guo

et al. 2021

Bioresource Technology

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“…However, enzymatic hydrolysis is preferred due to its advantages of controllable, reproducible, as well as retaining the nutritional value of the product and resulting in fewer unwanted side-effects and by-products (Halim et al, 2016;Hathwar et al, 2011;Tarté, 2009). Furthermore, enzyme catalysis has more advantages in degrading fats and protein than physicochemical catalysis because of its mild, highly efficient, eco-friendly reaction and catalytic specificity as well, without affecting other nutrients in the sample (Brandelli et al, 2015;Yao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Sustainable enzymatic technologies in waste animal fat and protein management

Cheng

Liu

Ngo

et al. 2021

Journal of Environmental Management

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Waste animal fats and proteins (WAFP) are rich in various animal by-products from food industries. On one hand, increasing production of huge amounts of WAFP brings a great challenge to their appropriate disposal, and raises severe risks to environment and life health. On the other hand, the high fat and protein contents in these animal wastes are valuable resources which can be reutilized in an eco-friendly and renewable way. Sustainable enzymatic technologies are promising methods for WAFP management. This review discussed the application of various enzymes in the conversion of WSFP to value-added biodiesel and bioactivate hydrolysates. New biotechnologies to discover novel enzymes with robust properties were proposed as well. This paper also presented the bio-utilization strategy of animal fat and protein wastes as alternative nutrient media for microorganism growth activities to yield important industrial enzymes cost-effectively.

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“…Recovery of valuable organic components from secondary meat and bone raw materials is based on their destruction, which is performed by different methods. The main methods for degradation of highly mineralized raw materials are based on tough hydrolysis, which is carried out using chemical reagents (acids, alkali) as well as by enzymatic, thermal or combined ways [2,17,18,19,20,21,22,23]. However, acids and alkali destroy several valuable amino acids, reduce the biological value of proteins and peptides, hydrolyse lipids.…”

Section: Introductionmentioning

confidence: 99%

“…Despite available scientific technological developments, the question of the full and effective use of the biological potential of meat and bone raw materials has no economically efficient solution up to now. Proposed processing methods allow recovering the most valuable protein fraction from different collagen raw materials at a level of 50-60% of the weight of contained protein; with that, the complex and non-waste processing is not achieved [13,21,23].…”

Section: Introductionmentioning

confidence: 99%

“…Deep enzymatic thermal hydrolysis enables obtaining low molecular weight peptides (1-100 kDa) from collagen-containing animal tissues. The most valuable is the fraction with a molecular weight of 1-10 kDa, which is assigned to the so-called active peptides and comprises di-, tri-, and tetra-peptides with novel physiological properties [23]. It was established that low molecular weight peptides of animal origin are effective antioxidants, antiseptics, have the immunomodulatory, hypotensive, regenerative, reproductive activities, exert the cytotoxic effect on several cancer cell lines, inhibit angiotensin-converting enzymes [11,12,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

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Obtaining and estimating the potential of protein nutraceuticals from highly mineralized collagen-containing beef raw materials

Mezenova

Агафонова

Мезенова

et al. 2021

Teor. prakt. pererab. mâsa (Print)

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Highly mineralized collagen-containing beef raw materials (tibia, fibula and costal bones) are a source of valuable protein nutraceuticals. They include high molecular weight proteins, oligopeptides and amino acids, which anabolic and physiological potential is used insufficiently. Protein nutraceuticals were obtained by high-temperature hydrolysis of beef raw materials in combination with enzymolysis by proteolytic enzyme preparations Alcalase 2,5 L, Protamex, Protosubtilin G3x. The water-soluble fraction of hydrolysates was studied after its separation and freeze-drying on the content of nitrogenous compounds, fats, minerals, formol-titrated nitrogen, fractional molecular composition. The mathematical dependencies of accumulation of low molecular weight products of protein hydrolysis on enzymolysis duration and doses of different enzyme preparations were obtained. The rational technological scheme of complex processing of beef raw materials with production of protein, fat and mineral-protein additives was proposed. The protein weight fraction in the freeze-dried protein hydrolysates was 69.5–89.6%. All studied protein additives contained peptides with a molecular weight of not more than 100 kDa. The content of low-molecular weight oligopeptides with a molecular weight of less than 10 kDa in the protein additives obtained by enzymatic thermal hydrolysis was more than 90%. The amino acid composition of protein additives produced by different hydrolytic methods was analyzed. Sensory and functional-technological properties of freeze-dried protein compositions were studied. The amino acid potential, high assimilability and physiological activity of protein nutraceuticals from collagen-containing beef raw materials were established. It is recommended to use them in the composition of specialized biologically active additives (BAAs) to food of the osteotropic direction in recipes of specialized and personalized products as a source of amino acids and active peptides.

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Insights into the improvement of the enzymatic hydrolysis of bovine bone protein using lipase pretreatment

Cited by 32 publications

References 29 publications

Roles and applications of enzymes for resistant pollutants removal in wastewater treatment

Roles and applications of enzymes for resistant pollutants removal in wastewater treatment

Sustainable enzymatic technologies in waste animal fat and protein management

Obtaining and estimating the potential of protein nutraceuticals from highly mineralized collagen-containing beef raw materials

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