Insights on proximity effect and multiphoton induced luminescence from gold nanospheres in far field optical microscopy

Borglin, Johan; Guldbrand, Stina; Evenbratt, Hanne; Kirejev, Vladimir; Grönbeck, Henrik; Ericson, Marica B.

doi:10.1063/1.4936554

Cited by 11 publications

(19 citation statements)

References 45 publications

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“…This in turn increases the probability of a sequential two-photon absorption process when illuminated using focused NIR light from femto-second-pulsed lasers, operating in the tissue transparent wavelength region. [55,56] The resulting multiphoton-induced luminescence (MIL) of the compressed BC-AuNP membranes could be clearly visualized in far-field multiphoton laser scanning microscopy (MPM) (Figure 7a,b and Figure S15, Supporting Information). In contrast, the noncompressed membranes showed no MIL signal (Figure 7a, inset).…”

Section: Nonlinear Optical Effects and Plasmonic Heatingmentioning

confidence: 99%

Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

Eskilson

Lindström

Sepúlveda

et al. 2020

Adv Funct Materials

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Nanocomposites of metal nanoparticles (NPs) and bacterial nanocellulose (BC) enable fabrication of soft and biocompatible materials for optical, catalytic, electronic, and biomedical applications. Current BC-NP nanocomposites are typically prepared by in situ synthesis of the NPs or electrostatic adsorption of surface functionalized NPs, which limits possibilities to control and tune NP size, shape, concentration, and surface chemistry and influences the properties and performance of the materials. Here a self-assembly strategy is described for fabrication of complex and well-defined BC-NP composites using colloidal gold and silver NPs of different sizes, shapes, and concentrations. The self-assembly process results in nanocomposites with distinct biophysical and optical properties. In addition to antibacterial materials and materials with excellent senor performance, materials with unique mechanoplasmonic properties are developed. The homogenous incorporation of plasmonic gold NPs in the BC enables extensive modulation of the optical properties by mechanical stimuli. Compression gives rise to near-field coupling between adsorbed NPs, resulting in tunable spectral variations and enhanced broadband absorption that amplify both nonlinear optical and thermoplasmonic effects and enables novel biosensing strategies.

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Section: Nonlinear Optical Effects and Plasmonic Heatingmentioning

confidence: 99%

Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

Eskilson

Lindström

Sepúlveda

et al. 2020

Adv Funct Materials

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“…The particles are deposited onto different oxide and metal surfaces such as vanadium (V/V 2 O 5 ), silicon dioxide (Si/SiO 2 ), gold (Au), aluminum (Al/Al 2 O 3 ), copper (Cu/CuO) and nickel (Ni/NiO 2 ). The reason for choosing citrate stabilized NPs is due to their availability and broad use in many applications [8,9]. Due to the citrate ligand, further functionalization with functional molecules such as proteins are considered to be straightforward [21,22].…”

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confidence: 99%

Guided selective deposition of nanoparticles by tuning of the surface potential

Eklöf¹,

Stolaś²,

Herzberg³

et al. 2017

EPL

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Guided deposition of nanoparticles onto different substrates is of great importance for a variety of applications such as biosensing, targeted cancer therapy, anti-bacterial coatings and single molecular electronics. It is therefore important to gain an understanding of what parameters are involved in the deposition of nanoparticles. In this work we have deposited 60 nm, negatively charged, citrate stabilized gold nanoparticles onto microstructures consisting of six different materials, (vanadium (V), silicon dioxide (SiO2), gold (Au), aluminum (Al), copper (Cu) and nickel (Ni)). The samples have then been investigated by scanning electron microscopy to extract the particle density. The surface potential was calculated from the measured surface charge density maps measured by atomic force microscopy while the samples were submerged in a KCl water solution. These values were compared with literature values of the isoelectric points (IEP) of different oxides formed on the metals in an ambient environment. According to measurements, Al had the highest surface potential followed by Ni and Cu. The same trend was observed for the nanoparticle densities. No particles were found on V, SiO2 and Au. The literature values of the IEP showed a different trend compared to the surface potential measurements concluding that IEP is not a reliable parameter for the prediction of NP deposition.

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“…However, we found that this approach provided only low image contrast due to a relatively strong background scattering from cellular organelles (data not shown). Previously, it was shown that gold nanoparticles exhibit a strong, shape-dependent TPL − that can be used for high-contrast imaging of AuNPs in cells . Therefore, we explored the technique of TPL in which AuNPs are induced to emit light upon two-photon absorption of light from a femtosecond-pulsed laser.…”

Section: Resultsmentioning

confidence: 99%

Fate of Antibody-Targeted Ultrasmall Gold Nanoparticles in Cancer Cells after Receptor-Mediated Uptake

2021

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Nanoparticles with ultrasmall sizes (less than 10 nm) offer many advantages in biomedical applications compared to their bigger counterparts, including better intratumoral distribution, improved pharmacokinetics (PK), and efficient body clearance. When functionalized with a biocompatible coating and a target-specific antibody, ultrasmall nanoparticles represent an attractive clinical translation platform. Although there is a tremendous body of work dedicated to PK and the biological effects of various nanoparticles, little is known about the fate of different components of functionalized nanoparticles in a biological environment such as in live cells. Here, we used luminescence properties of 5 nm gold nanoparticles (AuNPs) to study the intracellular trafficking and fate of the AuNPs functionalized with an organic layer consisting of a polyethylene glycol (PEG) coating and epidermal growth factor receptor (EGFR)-targeting antibody. We showed that intracellular uptake of the targeted 5 nm AuNPs results in a strong two-photon luminescence (TPL) that is characterized by broad emission and very short lifetimes compared to the fluorescence of the nanoparticle-conjugated fluorophore-tagged antibody, thereby allowing selective imaging of these components using TPL and two-photon excited fluorescence lifetime microscopy (2P-FLIM). Our results indicate that the nanoparticle’s coating is detached from the particle’s surface inside cells, leading to formation of nanoparticle clusters with a strong TPL. Furthermore, we observed an optically resolved spatial separation of the gold core and the antibody coating of the particles inside cells. We used data from two-photon microscopy, 2P-FLIM, electron microscopy, and in vitro assays to propose a model of interactions of functionalized 5 nm AuNPs with live cells.

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Insights on proximity effect and multiphoton induced luminescence from gold nanospheres in far field optical microscopy

Cited by 11 publications

References 45 publications

Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

Guided selective deposition of nanoparticles by tuning of the surface potential

Fate of Antibody-Targeted Ultrasmall Gold Nanoparticles in Cancer Cells after Receptor-Mediated Uptake

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