Insulin‐like growth factor‐I promotes resistance of bovine preimplantation embryos to heat shock through actions independent of its anti‐apoptotic actions requiring PI3K signaling

Jousan, Frank Dean; Hansen, Peter J.

doi:10.1002/mrd.20527

Cited by 90 publications

(68 citation statements)

References 37 publications

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“…These findings supported our present data where a similar pattern, i.e., a remarkably decreased (p<0.001) percentage of buffalo oocytes that developed to 2, 4, 8-16 cell, morulae or blastocyst stage was observed. Moreover, studies carried out in bovines have also shown the similar results, for example, it has been reported that exposure of bovine oocytes to increased temperature (40-41°C for 12 h) reduced the development of oocytes to blastocysts, the number of embryos developing to the blastocyst stage, 4-8 cell embryos, percentage of cleaved embryos and blastocysts [19,26,27]. In a similar attempt, the reduced quality of buffalo oocytes was reported in summer and autumn compared to winter and spring [28].…”

Section: Discussionsupporting

confidence: 55%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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Objective To determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5°C) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. Method The control group oocytes were cultured at 38.5°C for 24 h. The treatment 1 (T1) and 3 (T3) group oocytes were cultured at 40.5 and 41.5°C respectively, for the first 12 h and at 38.5°C for rest of the 12 h. However, treatment 2 (T2) and 4 (T4) group oocytes were cultured at 40.5 and 41.5°C for complete 24 h. Results Development of oocytes to blastocyst was severely compromised (p<0.001) when matured at 40.5 and 41.5°C for both exposure periods (12 h and 24 h). It was found that the cleavage rates, blastocyst yield and mean cell number decreased remarkably (p<0.001) in the treatment groups compared to control. The relative mRNA expression of heat shock protein (Hsp 70.1, 70.2, 70.8, 60, 10 and HSF1), pro-apoptotic (caspases-3, −7, −8, Bid and Bax) and oxidative stress (iNOS) related genes was significantly higher (p<0.05) in all the treatment groups compared to control. However, mRNA abundance of anti-apoptotic (Bcl-2, Mcl-1, Bcl-xl), glucose transport (Glut1, Glut3 and IGF1R), developmental competence (ZAR1 and BMP15) and oxidative stress (MnSOD) related genes was significantly decreased (p<0.05) in the treatment groups compared to control. Conclusion The present study clearly establishes that physiologically relevant elevated temperatures during in vitro meiotic maturation reduce developmental competence of bubaline oocytes.

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Section: Discussionsupporting

confidence: 55%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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“…Also, several studies reported that IGF-I promotes pre-implantation embryo development and cell numbers in many species, including mouse [15], cow [18], and human [17]. The antiapoptotic effects of IGF-I generally act through a phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway which subsequently inactivates pro-apoptotic proteins such as Bad and caspase-9 and stimulates anti-apoptotic proteins, including Bcl-2, a number of transcription factors and translational regulatory proteins [41,42]. Therefore, IGF-I may promote embryonic development by an anti-apoptotic effect instead of increasing HIF-1α expression.…”

Section: Discussionmentioning

confidence: 99%

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Yoon

Juhn

et al. 2012

J Assist Reprod Genet

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Purpose Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O 2 in both the absence and presence of IGF-I. Results The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O 2 exhibited significantly higher total cell numbers (83.4±18.1) and lower apoptotic index (3.7±1.5) than those cultured under 20 % O 2 (67.4±15.6) (6.9±3.5) (P<0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-apoptotic effect, instead of increasing HIF-1α protein expression.

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“…Culture with IGF1 increases the percent of embryos capable of developing to the blastocyst stage (28,30,(64)(65)(66)(67)(68)(69). This action of IGF1 probably involves increased proliferation because the cell number of day 6 morulae was increased by IGF1 (70). Effects on development could be blocked with antibody to IGF1R (64) and an inhibitor of MAPK activation (69,70).…”

Section: Insulin-like Growth Factormentioning

confidence: 99%

Maternal embryokines that regulate development of the bovine preimplantation embryo

Hansen¹,

Denicol²,

Dobbs³

2014

Turk J Vet Anim Sci

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The zygote contains within its genome and epigenome the program to direct development through the embryonic, fetal, and postnatal periods. The execution of that program is dependent on the embryo's nongenetic inheritance from the oocyte and sperm (1,2) as well as on the environment in which development proceeds. Variation in the maternal environment can affect the ability of the preimplantation embryo to establish pregnancy. In cattle, the focus of this review, competence of the preimplantation embryo for growth and survival can be affected by maternal parity (3), lactational status (4), and circulating concentrations of progesterone (5). Moreover, patterns of gene expression in the endometrium are associated with survival of an embryo transferred to the uterus in the next ovulatory cycle (6,7).Development of the bovine embryo to the blastocyst in the absence of maternal signals (i.e. through in vitro production procedures) results in embryos with aberrant gene expression (8,9), lipid content (10,11), DNA methylation (12), and, as shown in the Table, reduced competence to establish pregnancy after transfer into recipients (13-17). Additionally, an increased proportion of embryos produced in vitro have developmental abnormalities that lead to increased neonatal death losses (18-20). Some of the problems with the in vitro-produced embryo could result from selection of incompetent oocytes or inadequate oocyte maturation. However, the importance of the maternal environment during development is indicated by observations that transfer of in vitro-produced embryos to the oviduct after fertilization limits some of the abnormalities associated with in vitro production (9,21).One function of the reproductive tract is to secrete bioactive molecules that regulate the embryo, oviduct, or endometrium. Genes for 115 ligands expressed in the endometrium had the corresponding receptor gene expressed by the embryo (22). Regulatory molecules produced by the oviduct and endometrium, which include hormones, growth factors, and cytokines, are referred to as embryokines when they function to regulate embryonic growth and development (23).It is likely that some of the variation in the ability of the reproductive tract to support embryonic development represents variation in secretion of embryokines. Indeed, several genes that were overexpressed in the endometrium of cows that subsequently became pregnant after embryo transfer as compared to cows that did not establish pregnancy are potential embryokines. These include NGF,

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Insulin‐like growth factor‐I promotes resistance of bovine preimplantation embryos to heat shock through actions independent of its anti‐apoptotic actions requiring PI3K signaling

Cited by 90 publications

References 37 publications

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Maternal embryokines that regulate development of the bovine preimplantation embryo

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